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JBP485 promotes tear and mucin secretion in ocular surface epithelia.

Nakamura T, Hata Y, Nagata M, Yokoi N, Yamaguchi S, Kaku T, Kinoshita S - Sci Rep (2015)

Bottom Line: Tear film contains ocular mucins and is essential for maintaining the homeostasis of the wet ocular surface.Since there are a limited number of clinical options for the treatment of DES, additional novel treatments are needed to improve the clinical results.Moreover, JBP485 clinically improved corneal epithelial damage in a mouse dry eye model.

View Article: PubMed Central - PubMed

Affiliation: 1] Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan [2] Research Center for Inflammation and Regenerative Medicine, Doshisha University, Kyoto, Japan.

ABSTRACT
Dry eye syndrome (DES), a multifactorial disease of the tears and ocular surface, is one of the most common ocular disorders. Tear film contains ocular mucins and is essential for maintaining the homeostasis of the wet ocular surface. Since there are a limited number of clinical options for the treatment of DES, additional novel treatments are needed to improve the clinical results. In this study, we found that placental extract-derived dipeptide (JBP485) clearly promoted the expression and secretion of gel-forming mucin 5ac (Muc5ac) in rabbit conjunctival epithelium. JBP485 also elevated the expression level of cell surface-associated mucins (Muc1/4/16) in rabbit corneal epithelium. The Schirmer tear test results indicated that JBP485 induced tear secretion in the rabbit model. Moreover, JBP485 clinically improved corneal epithelial damage in a mouse dry eye model. Thus, our data indicate that JBP485 efficiently promoted mucin and aqueous tear secretion in rabbit ocular surface epithelium and has the potential to be used as a novel treatment for DES.

No MeSH data available.


Related in: MedlinePlus

JBP485 induces tear secretion in a rabbit model.Schirmer test scores at different time points (up to 30 minutes) after the topical application of JBP485 solution (A, n = 8–9). Schirmer test scores for different concentrations of JBP485 after the topical application of JBP485 solution (B, n = 8-10). ** p < 0.01.
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f7: JBP485 induces tear secretion in a rabbit model.Schirmer test scores at different time points (up to 30 minutes) after the topical application of JBP485 solution (A, n = 8–9). Schirmer test scores for different concentrations of JBP485 after the topical application of JBP485 solution (B, n = 8-10). ** p < 0.01.

Mentions: To investigate the effect of JBP485 on aqueous tear-fluid secretion, we performed the Schirmer test using a rabbit model. Topical application of JBP485 (100 μM) significantly increased aqueous tear volume from 5 minutes post application, and that effect gradually decreased between 5 and 30 minutes post application (Fig. 7A). Next, we examined the effect of different concentrations of JBP485 and found that JBP485 clearly increased aqueous tear volume in a concentration-dependent manner (up to 10 mM) in rabbit eyes (Fig. 7B). These results confirm that JBP485 accelerates tear secretion in a rabbit model.


JBP485 promotes tear and mucin secretion in ocular surface epithelia.

Nakamura T, Hata Y, Nagata M, Yokoi N, Yamaguchi S, Kaku T, Kinoshita S - Sci Rep (2015)

JBP485 induces tear secretion in a rabbit model.Schirmer test scores at different time points (up to 30 minutes) after the topical application of JBP485 solution (A, n = 8–9). Schirmer test scores for different concentrations of JBP485 after the topical application of JBP485 solution (B, n = 8-10). ** p < 0.01.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4440520&req=5

f7: JBP485 induces tear secretion in a rabbit model.Schirmer test scores at different time points (up to 30 minutes) after the topical application of JBP485 solution (A, n = 8–9). Schirmer test scores for different concentrations of JBP485 after the topical application of JBP485 solution (B, n = 8-10). ** p < 0.01.
Mentions: To investigate the effect of JBP485 on aqueous tear-fluid secretion, we performed the Schirmer test using a rabbit model. Topical application of JBP485 (100 μM) significantly increased aqueous tear volume from 5 minutes post application, and that effect gradually decreased between 5 and 30 minutes post application (Fig. 7A). Next, we examined the effect of different concentrations of JBP485 and found that JBP485 clearly increased aqueous tear volume in a concentration-dependent manner (up to 10 mM) in rabbit eyes (Fig. 7B). These results confirm that JBP485 accelerates tear secretion in a rabbit model.

Bottom Line: Tear film contains ocular mucins and is essential for maintaining the homeostasis of the wet ocular surface.Since there are a limited number of clinical options for the treatment of DES, additional novel treatments are needed to improve the clinical results.Moreover, JBP485 clinically improved corneal epithelial damage in a mouse dry eye model.

View Article: PubMed Central - PubMed

Affiliation: 1] Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan [2] Research Center for Inflammation and Regenerative Medicine, Doshisha University, Kyoto, Japan.

ABSTRACT
Dry eye syndrome (DES), a multifactorial disease of the tears and ocular surface, is one of the most common ocular disorders. Tear film contains ocular mucins and is essential for maintaining the homeostasis of the wet ocular surface. Since there are a limited number of clinical options for the treatment of DES, additional novel treatments are needed to improve the clinical results. In this study, we found that placental extract-derived dipeptide (JBP485) clearly promoted the expression and secretion of gel-forming mucin 5ac (Muc5ac) in rabbit conjunctival epithelium. JBP485 also elevated the expression level of cell surface-associated mucins (Muc1/4/16) in rabbit corneal epithelium. The Schirmer tear test results indicated that JBP485 induced tear secretion in the rabbit model. Moreover, JBP485 clinically improved corneal epithelial damage in a mouse dry eye model. Thus, our data indicate that JBP485 efficiently promoted mucin and aqueous tear secretion in rabbit ocular surface epithelium and has the potential to be used as a novel treatment for DES.

No MeSH data available.


Related in: MedlinePlus