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JBP485 promotes tear and mucin secretion in ocular surface epithelia.

Nakamura T, Hata Y, Nagata M, Yokoi N, Yamaguchi S, Kaku T, Kinoshita S - Sci Rep (2015)

Bottom Line: Tear film contains ocular mucins and is essential for maintaining the homeostasis of the wet ocular surface.Since there are a limited number of clinical options for the treatment of DES, additional novel treatments are needed to improve the clinical results.Moreover, JBP485 clinically improved corneal epithelial damage in a mouse dry eye model.

View Article: PubMed Central - PubMed

Affiliation: 1] Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan [2] Research Center for Inflammation and Regenerative Medicine, Doshisha University, Kyoto, Japan.

ABSTRACT
Dry eye syndrome (DES), a multifactorial disease of the tears and ocular surface, is one of the most common ocular disorders. Tear film contains ocular mucins and is essential for maintaining the homeostasis of the wet ocular surface. Since there are a limited number of clinical options for the treatment of DES, additional novel treatments are needed to improve the clinical results. In this study, we found that placental extract-derived dipeptide (JBP485) clearly promoted the expression and secretion of gel-forming mucin 5ac (Muc5ac) in rabbit conjunctival epithelium. JBP485 also elevated the expression level of cell surface-associated mucins (Muc1/4/16) in rabbit corneal epithelium. The Schirmer tear test results indicated that JBP485 induced tear secretion in the rabbit model. Moreover, JBP485 clinically improved corneal epithelial damage in a mouse dry eye model. Thus, our data indicate that JBP485 efficiently promoted mucin and aqueous tear secretion in rabbit ocular surface epithelium and has the potential to be used as a novel treatment for DES.

No MeSH data available.


Related in: MedlinePlus

JBP485 elevates the expression level of cell surface-associated mucin in in vivo corneal epithelium.Relative expression of mucin-related molecules (Muc1/4/16 and Galectin-3) in the control- and JBP485-treated in vivo corneal epithelium (A–D). ctrl: control (n = 3).
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f6: JBP485 elevates the expression level of cell surface-associated mucin in in vivo corneal epithelium.Relative expression of mucin-related molecules (Muc1/4/16 and Galectin-3) in the control- and JBP485-treated in vivo corneal epithelium (A–D). ctrl: control (n = 3).

Mentions: Finally, the effect of JBP485 on corneal CECs was investigated by examining the expression level of cell surface-associated mucin related molecules (Muc1/4/16 and Galectin-3) in in vivo CECs. Briefly, JBP485 (100 μM) or saline solution was topically applied 4-times daily for 12 days, and then the CECs were obtained by mechanical scraping. As expected, the expression levels of Muc1/4/16 and Galectin-3 in the JBP485-treated CECs tended to be higher than in the controls (Fig. 6A–D). These findings clearly indicate that JBP485 promotes the expression level of cell surface-associated mucins in CECs.


JBP485 promotes tear and mucin secretion in ocular surface epithelia.

Nakamura T, Hata Y, Nagata M, Yokoi N, Yamaguchi S, Kaku T, Kinoshita S - Sci Rep (2015)

JBP485 elevates the expression level of cell surface-associated mucin in in vivo corneal epithelium.Relative expression of mucin-related molecules (Muc1/4/16 and Galectin-3) in the control- and JBP485-treated in vivo corneal epithelium (A–D). ctrl: control (n = 3).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4440520&req=5

f6: JBP485 elevates the expression level of cell surface-associated mucin in in vivo corneal epithelium.Relative expression of mucin-related molecules (Muc1/4/16 and Galectin-3) in the control- and JBP485-treated in vivo corneal epithelium (A–D). ctrl: control (n = 3).
Mentions: Finally, the effect of JBP485 on corneal CECs was investigated by examining the expression level of cell surface-associated mucin related molecules (Muc1/4/16 and Galectin-3) in in vivo CECs. Briefly, JBP485 (100 μM) or saline solution was topically applied 4-times daily for 12 days, and then the CECs were obtained by mechanical scraping. As expected, the expression levels of Muc1/4/16 and Galectin-3 in the JBP485-treated CECs tended to be higher than in the controls (Fig. 6A–D). These findings clearly indicate that JBP485 promotes the expression level of cell surface-associated mucins in CECs.

Bottom Line: Tear film contains ocular mucins and is essential for maintaining the homeostasis of the wet ocular surface.Since there are a limited number of clinical options for the treatment of DES, additional novel treatments are needed to improve the clinical results.Moreover, JBP485 clinically improved corneal epithelial damage in a mouse dry eye model.

View Article: PubMed Central - PubMed

Affiliation: 1] Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan [2] Research Center for Inflammation and Regenerative Medicine, Doshisha University, Kyoto, Japan.

ABSTRACT
Dry eye syndrome (DES), a multifactorial disease of the tears and ocular surface, is one of the most common ocular disorders. Tear film contains ocular mucins and is essential for maintaining the homeostasis of the wet ocular surface. Since there are a limited number of clinical options for the treatment of DES, additional novel treatments are needed to improve the clinical results. In this study, we found that placental extract-derived dipeptide (JBP485) clearly promoted the expression and secretion of gel-forming mucin 5ac (Muc5ac) in rabbit conjunctival epithelium. JBP485 also elevated the expression level of cell surface-associated mucins (Muc1/4/16) in rabbit corneal epithelium. The Schirmer tear test results indicated that JBP485 induced tear secretion in the rabbit model. Moreover, JBP485 clinically improved corneal epithelial damage in a mouse dry eye model. Thus, our data indicate that JBP485 efficiently promoted mucin and aqueous tear secretion in rabbit ocular surface epithelium and has the potential to be used as a novel treatment for DES.

No MeSH data available.


Related in: MedlinePlus