Limits...
JBP485 promotes tear and mucin secretion in ocular surface epithelia.

Nakamura T, Hata Y, Nagata M, Yokoi N, Yamaguchi S, Kaku T, Kinoshita S - Sci Rep (2015)

Bottom Line: Tear film contains ocular mucins and is essential for maintaining the homeostasis of the wet ocular surface.Since there are a limited number of clinical options for the treatment of DES, additional novel treatments are needed to improve the clinical results.Moreover, JBP485 clinically improved corneal epithelial damage in a mouse dry eye model.

View Article: PubMed Central - PubMed

Affiliation: 1] Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan [2] Research Center for Inflammation and Regenerative Medicine, Doshisha University, Kyoto, Japan.

ABSTRACT
Dry eye syndrome (DES), a multifactorial disease of the tears and ocular surface, is one of the most common ocular disorders. Tear film contains ocular mucins and is essential for maintaining the homeostasis of the wet ocular surface. Since there are a limited number of clinical options for the treatment of DES, additional novel treatments are needed to improve the clinical results. In this study, we found that placental extract-derived dipeptide (JBP485) clearly promoted the expression and secretion of gel-forming mucin 5ac (Muc5ac) in rabbit conjunctival epithelium. JBP485 also elevated the expression level of cell surface-associated mucins (Muc1/4/16) in rabbit corneal epithelium. The Schirmer tear test results indicated that JBP485 induced tear secretion in the rabbit model. Moreover, JBP485 clinically improved corneal epithelial damage in a mouse dry eye model. Thus, our data indicate that JBP485 efficiently promoted mucin and aqueous tear secretion in rabbit ocular surface epithelium and has the potential to be used as a novel treatment for DES.

No MeSH data available.


Related in: MedlinePlus

JBP485 elevates the expression level of cell surface-associated mucin in ex vivo corneal epithelium. Relative expression of mucin-related molecules (Muc1/4/16 and Galectin-3) in the control- and JBP485-treated ex vivo corneal epithelium (A–D). ctrl: control. * p < 0.05 (n = 2).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4440520&req=5

f5: JBP485 elevates the expression level of cell surface-associated mucin in ex vivo corneal epithelium. Relative expression of mucin-related molecules (Muc1/4/16 and Galectin-3) in the control- and JBP485-treated ex vivo corneal epithelium (A–D). ctrl: control. * p < 0.05 (n = 2).

Mentions: To further investigate the effect of JBP485 on CECs, we examined the expression level of cell surface-associated mucin related molecules (Muc1/4/16 and Galectin-3) in ex vivo CECs. Briefly, whole rabbit eyes were incubated in JBP485 (100 uM), and CECs were then collected by mechanical scraping at 4 time points (after 3, 6, 12, and 24 hours of incubation). Real-time PCR showed that after incubation with JBP485 for 3–6 ours, the relative mRNA expression of Muc1/4/16 in ex vivo CECs tended to be higher than that in the control samples (Fig. 5A–D).


JBP485 promotes tear and mucin secretion in ocular surface epithelia.

Nakamura T, Hata Y, Nagata M, Yokoi N, Yamaguchi S, Kaku T, Kinoshita S - Sci Rep (2015)

JBP485 elevates the expression level of cell surface-associated mucin in ex vivo corneal epithelium. Relative expression of mucin-related molecules (Muc1/4/16 and Galectin-3) in the control- and JBP485-treated ex vivo corneal epithelium (A–D). ctrl: control. * p < 0.05 (n = 2).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4440520&req=5

f5: JBP485 elevates the expression level of cell surface-associated mucin in ex vivo corneal epithelium. Relative expression of mucin-related molecules (Muc1/4/16 and Galectin-3) in the control- and JBP485-treated ex vivo corneal epithelium (A–D). ctrl: control. * p < 0.05 (n = 2).
Mentions: To further investigate the effect of JBP485 on CECs, we examined the expression level of cell surface-associated mucin related molecules (Muc1/4/16 and Galectin-3) in ex vivo CECs. Briefly, whole rabbit eyes were incubated in JBP485 (100 uM), and CECs were then collected by mechanical scraping at 4 time points (after 3, 6, 12, and 24 hours of incubation). Real-time PCR showed that after incubation with JBP485 for 3–6 ours, the relative mRNA expression of Muc1/4/16 in ex vivo CECs tended to be higher than that in the control samples (Fig. 5A–D).

Bottom Line: Tear film contains ocular mucins and is essential for maintaining the homeostasis of the wet ocular surface.Since there are a limited number of clinical options for the treatment of DES, additional novel treatments are needed to improve the clinical results.Moreover, JBP485 clinically improved corneal epithelial damage in a mouse dry eye model.

View Article: PubMed Central - PubMed

Affiliation: 1] Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan [2] Research Center for Inflammation and Regenerative Medicine, Doshisha University, Kyoto, Japan.

ABSTRACT
Dry eye syndrome (DES), a multifactorial disease of the tears and ocular surface, is one of the most common ocular disorders. Tear film contains ocular mucins and is essential for maintaining the homeostasis of the wet ocular surface. Since there are a limited number of clinical options for the treatment of DES, additional novel treatments are needed to improve the clinical results. In this study, we found that placental extract-derived dipeptide (JBP485) clearly promoted the expression and secretion of gel-forming mucin 5ac (Muc5ac) in rabbit conjunctival epithelium. JBP485 also elevated the expression level of cell surface-associated mucins (Muc1/4/16) in rabbit corneal epithelium. The Schirmer tear test results indicated that JBP485 induced tear secretion in the rabbit model. Moreover, JBP485 clinically improved corneal epithelial damage in a mouse dry eye model. Thus, our data indicate that JBP485 efficiently promoted mucin and aqueous tear secretion in rabbit ocular surface epithelium and has the potential to be used as a novel treatment for DES.

No MeSH data available.


Related in: MedlinePlus