Limits...
JBP485 promotes tear and mucin secretion in ocular surface epithelia.

Nakamura T, Hata Y, Nagata M, Yokoi N, Yamaguchi S, Kaku T, Kinoshita S - Sci Rep (2015)

Bottom Line: Tear film contains ocular mucins and is essential for maintaining the homeostasis of the wet ocular surface.Since there are a limited number of clinical options for the treatment of DES, additional novel treatments are needed to improve the clinical results.Moreover, JBP485 clinically improved corneal epithelial damage in a mouse dry eye model.

View Article: PubMed Central - PubMed

Affiliation: 1] Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan [2] Research Center for Inflammation and Regenerative Medicine, Doshisha University, Kyoto, Japan.

ABSTRACT
Dry eye syndrome (DES), a multifactorial disease of the tears and ocular surface, is one of the most common ocular disorders. Tear film contains ocular mucins and is essential for maintaining the homeostasis of the wet ocular surface. Since there are a limited number of clinical options for the treatment of DES, additional novel treatments are needed to improve the clinical results. In this study, we found that placental extract-derived dipeptide (JBP485) clearly promoted the expression and secretion of gel-forming mucin 5ac (Muc5ac) in rabbit conjunctival epithelium. JBP485 also elevated the expression level of cell surface-associated mucins (Muc1/4/16) in rabbit corneal epithelium. The Schirmer tear test results indicated that JBP485 induced tear secretion in the rabbit model. Moreover, JBP485 clinically improved corneal epithelial damage in a mouse dry eye model. Thus, our data indicate that JBP485 efficiently promoted mucin and aqueous tear secretion in rabbit ocular surface epithelium and has the potential to be used as a novel treatment for DES.

No MeSH data available.


Related in: MedlinePlus

JBP485 accelerates the secretion of Muc5ac in conjunctival epithelium (in vivo).Representative Periodic Acid Schiff staining of conjunctival epithelium 30 minutes after a topical application of saline solution (A) and JBP485 (B). Protein levels of Muc5ac in the JBP485-treated (100 μM) conjunctival epithelium were examined by enzyme-linked immunosorbent assay (C). * p < 0.05 (n = 6). Scale bar, 100 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4440520&req=5

f3: JBP485 accelerates the secretion of Muc5ac in conjunctival epithelium (in vivo).Representative Periodic Acid Schiff staining of conjunctival epithelium 30 minutes after a topical application of saline solution (A) and JBP485 (B). Protein levels of Muc5ac in the JBP485-treated (100 μM) conjunctival epithelium were examined by enzyme-linked immunosorbent assay (C). * p < 0.05 (n = 6). Scale bar, 100 μm.

Mentions: To directly examine the secretion of Muc5ac from the CjECs, we performed Periodic Acid Schiff (PAS) staining (that can be used to detect secretory mucin) by use of impression cytology. Briefly, 30 minutes after the topical application of JBP485 or saline solution (control), the CjECs were obtained by use of impression cytology and the PAS staining was performed. We observed that PAS stained (+) cells were distributed in almost all areas of the eyes treated with saline solution, whereas the number of those cells (not goblet cells) decreased in the JBP485-treated eyes, indicating that JBP485 promotes mucin secretion (Fig. 3A, B).


JBP485 promotes tear and mucin secretion in ocular surface epithelia.

Nakamura T, Hata Y, Nagata M, Yokoi N, Yamaguchi S, Kaku T, Kinoshita S - Sci Rep (2015)

JBP485 accelerates the secretion of Muc5ac in conjunctival epithelium (in vivo).Representative Periodic Acid Schiff staining of conjunctival epithelium 30 minutes after a topical application of saline solution (A) and JBP485 (B). Protein levels of Muc5ac in the JBP485-treated (100 μM) conjunctival epithelium were examined by enzyme-linked immunosorbent assay (C). * p < 0.05 (n = 6). Scale bar, 100 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4440520&req=5

f3: JBP485 accelerates the secretion of Muc5ac in conjunctival epithelium (in vivo).Representative Periodic Acid Schiff staining of conjunctival epithelium 30 minutes after a topical application of saline solution (A) and JBP485 (B). Protein levels of Muc5ac in the JBP485-treated (100 μM) conjunctival epithelium were examined by enzyme-linked immunosorbent assay (C). * p < 0.05 (n = 6). Scale bar, 100 μm.
Mentions: To directly examine the secretion of Muc5ac from the CjECs, we performed Periodic Acid Schiff (PAS) staining (that can be used to detect secretory mucin) by use of impression cytology. Briefly, 30 minutes after the topical application of JBP485 or saline solution (control), the CjECs were obtained by use of impression cytology and the PAS staining was performed. We observed that PAS stained (+) cells were distributed in almost all areas of the eyes treated with saline solution, whereas the number of those cells (not goblet cells) decreased in the JBP485-treated eyes, indicating that JBP485 promotes mucin secretion (Fig. 3A, B).

Bottom Line: Tear film contains ocular mucins and is essential for maintaining the homeostasis of the wet ocular surface.Since there are a limited number of clinical options for the treatment of DES, additional novel treatments are needed to improve the clinical results.Moreover, JBP485 clinically improved corneal epithelial damage in a mouse dry eye model.

View Article: PubMed Central - PubMed

Affiliation: 1] Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan [2] Research Center for Inflammation and Regenerative Medicine, Doshisha University, Kyoto, Japan.

ABSTRACT
Dry eye syndrome (DES), a multifactorial disease of the tears and ocular surface, is one of the most common ocular disorders. Tear film contains ocular mucins and is essential for maintaining the homeostasis of the wet ocular surface. Since there are a limited number of clinical options for the treatment of DES, additional novel treatments are needed to improve the clinical results. In this study, we found that placental extract-derived dipeptide (JBP485) clearly promoted the expression and secretion of gel-forming mucin 5ac (Muc5ac) in rabbit conjunctival epithelium. JBP485 also elevated the expression level of cell surface-associated mucins (Muc1/4/16) in rabbit corneal epithelium. The Schirmer tear test results indicated that JBP485 induced tear secretion in the rabbit model. Moreover, JBP485 clinically improved corneal epithelial damage in a mouse dry eye model. Thus, our data indicate that JBP485 efficiently promoted mucin and aqueous tear secretion in rabbit ocular surface epithelium and has the potential to be used as a novel treatment for DES.

No MeSH data available.


Related in: MedlinePlus