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JBP485 promotes tear and mucin secretion in ocular surface epithelia.

Nakamura T, Hata Y, Nagata M, Yokoi N, Yamaguchi S, Kaku T, Kinoshita S - Sci Rep (2015)

Bottom Line: Tear film contains ocular mucins and is essential for maintaining the homeostasis of the wet ocular surface.Since there are a limited number of clinical options for the treatment of DES, additional novel treatments are needed to improve the clinical results.Moreover, JBP485 clinically improved corneal epithelial damage in a mouse dry eye model.

View Article: PubMed Central - PubMed

Affiliation: 1] Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan [2] Research Center for Inflammation and Regenerative Medicine, Doshisha University, Kyoto, Japan.

ABSTRACT
Dry eye syndrome (DES), a multifactorial disease of the tears and ocular surface, is one of the most common ocular disorders. Tear film contains ocular mucins and is essential for maintaining the homeostasis of the wet ocular surface. Since there are a limited number of clinical options for the treatment of DES, additional novel treatments are needed to improve the clinical results. In this study, we found that placental extract-derived dipeptide (JBP485) clearly promoted the expression and secretion of gel-forming mucin 5ac (Muc5ac) in rabbit conjunctival epithelium. JBP485 also elevated the expression level of cell surface-associated mucins (Muc1/4/16) in rabbit corneal epithelium. The Schirmer tear test results indicated that JBP485 induced tear secretion in the rabbit model. Moreover, JBP485 clinically improved corneal epithelial damage in a mouse dry eye model. Thus, our data indicate that JBP485 efficiently promoted mucin and aqueous tear secretion in rabbit ocular surface epithelium and has the potential to be used as a novel treatment for DES.

No MeSH data available.


Related in: MedlinePlus

JBP485 promotes the expression level of mucin-related molecules in conjunctival epithelium.Relative expression of mucin-related molecules [mucin 5ac (Muc5ac), Muc1/4/16, and Galectin-3] in the control- and JBP485-treated conjunctival epithelial cells (A–E, 4 cell membrane filters, mixed). ctrl: control.
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f1: JBP485 promotes the expression level of mucin-related molecules in conjunctival epithelium.Relative expression of mucin-related molecules [mucin 5ac (Muc5ac), Muc1/4/16, and Galectin-3] in the control- and JBP485-treated conjunctival epithelial cells (A–E, 4 cell membrane filters, mixed). ctrl: control.

Mentions: To investigate the effect of JBP485 on conjunctival epithelial cells, we first examined the expression level of secretory-mucin Muc5AC in rabbit conjunctival epithelial cells (CjECs) (ex vivo model). For this experiment, whole rabbit eyes were first incubated in the control solution [Hank’s balanced salt solution (HBSS)] and in JBP485 (100 μM) for 6 hours, and the CjECs were then collected using cell membrane filters from 4 different areas of the conjunctiva (the upper, lower, nasal, and temporal areas). The resultant 4-cell membrane filters were then mixed together and used for experiment. Interestingly, real-time polymerase chain reaction (PCR) tended to show an elevated level of Muc5ac expression in the JBP485-treated CjECs (Fig. 1A). In addition, we examined the expression of cell surface-associated mucin-related molecules (Muc1/4/16 and Galectin-3) and found that the expression levels of CjECs treated with JBP485 tended to be slightly increased compared to the controls (Fig. 1B–E). These findings suggest that JBP485 plays some critical role in inducing the expression of ocular surface mucins.


JBP485 promotes tear and mucin secretion in ocular surface epithelia.

Nakamura T, Hata Y, Nagata M, Yokoi N, Yamaguchi S, Kaku T, Kinoshita S - Sci Rep (2015)

JBP485 promotes the expression level of mucin-related molecules in conjunctival epithelium.Relative expression of mucin-related molecules [mucin 5ac (Muc5ac), Muc1/4/16, and Galectin-3] in the control- and JBP485-treated conjunctival epithelial cells (A–E, 4 cell membrane filters, mixed). ctrl: control.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4440520&req=5

f1: JBP485 promotes the expression level of mucin-related molecules in conjunctival epithelium.Relative expression of mucin-related molecules [mucin 5ac (Muc5ac), Muc1/4/16, and Galectin-3] in the control- and JBP485-treated conjunctival epithelial cells (A–E, 4 cell membrane filters, mixed). ctrl: control.
Mentions: To investigate the effect of JBP485 on conjunctival epithelial cells, we first examined the expression level of secretory-mucin Muc5AC in rabbit conjunctival epithelial cells (CjECs) (ex vivo model). For this experiment, whole rabbit eyes were first incubated in the control solution [Hank’s balanced salt solution (HBSS)] and in JBP485 (100 μM) for 6 hours, and the CjECs were then collected using cell membrane filters from 4 different areas of the conjunctiva (the upper, lower, nasal, and temporal areas). The resultant 4-cell membrane filters were then mixed together and used for experiment. Interestingly, real-time polymerase chain reaction (PCR) tended to show an elevated level of Muc5ac expression in the JBP485-treated CjECs (Fig. 1A). In addition, we examined the expression of cell surface-associated mucin-related molecules (Muc1/4/16 and Galectin-3) and found that the expression levels of CjECs treated with JBP485 tended to be slightly increased compared to the controls (Fig. 1B–E). These findings suggest that JBP485 plays some critical role in inducing the expression of ocular surface mucins.

Bottom Line: Tear film contains ocular mucins and is essential for maintaining the homeostasis of the wet ocular surface.Since there are a limited number of clinical options for the treatment of DES, additional novel treatments are needed to improve the clinical results.Moreover, JBP485 clinically improved corneal epithelial damage in a mouse dry eye model.

View Article: PubMed Central - PubMed

Affiliation: 1] Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan [2] Research Center for Inflammation and Regenerative Medicine, Doshisha University, Kyoto, Japan.

ABSTRACT
Dry eye syndrome (DES), a multifactorial disease of the tears and ocular surface, is one of the most common ocular disorders. Tear film contains ocular mucins and is essential for maintaining the homeostasis of the wet ocular surface. Since there are a limited number of clinical options for the treatment of DES, additional novel treatments are needed to improve the clinical results. In this study, we found that placental extract-derived dipeptide (JBP485) clearly promoted the expression and secretion of gel-forming mucin 5ac (Muc5ac) in rabbit conjunctival epithelium. JBP485 also elevated the expression level of cell surface-associated mucins (Muc1/4/16) in rabbit corneal epithelium. The Schirmer tear test results indicated that JBP485 induced tear secretion in the rabbit model. Moreover, JBP485 clinically improved corneal epithelial damage in a mouse dry eye model. Thus, our data indicate that JBP485 efficiently promoted mucin and aqueous tear secretion in rabbit ocular surface epithelium and has the potential to be used as a novel treatment for DES.

No MeSH data available.


Related in: MedlinePlus