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Prospective Study of (68)Ga-NOTA-NFB: Radiation Dosimetry in Healthy Volunteers and First Application in Glioma Patients.

Wang Z, Zhang M, Wang L, Wang S, Kang F, Li G, Jacobson O, Niu G, Yang W, Wang J, Chen X - Theranostics (2015)

Bottom Line: The expression of CXCR4 on the resected brain tumor tissues was determined by immunohistochemical staining. (68)Ga-NOTA-NFB was safe and well tolerated by all subjects.The mean effective dose was 25.4 ± 6.1 μSv/MBq.The histopathological staining confirmed that CXCR4 was overexpressed on resected tumor tissues with prominent (68)Ga-NOTA-NFB uptake.

View Article: PubMed Central - PubMed

Affiliation: 1. Department of Nuclear Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an, China.

ABSTRACT

Purpose: The chemokine receptor CXCR4 is overexpressed in various types of human cancers. As a specific imaging agent of CXCR4, (68)Ga-NOTA-NFB was investigated in this study to assess its safety, biodistribution and dosimetry properties in healthy volunteers, and to preliminarily evaluate its application in glioma patients.

Methods: Six healthy volunteers underwent whole-body PET scans at 0, 0.5, 1, 2 and 3 h after (68)Ga-NOTA-NFB injection (mean dose, 182.4 ± 3.7 MBq (4.93 ± 0.10 mCi)). For time-activity curve calculations, 1 mL blood samples were obtained at 1, 3, 5, 10, 30, 60, 90, 120, 150 and 180 min after the injection. The estimated radiation doses were calculated by OLINDA/EXM software. Eight patients with glioma were enrolled and underwent both (68)Ga-NOTA-NFB and (18)F-FDG PET/CT scans before surgery. The expression of CXCR4 on the resected brain tumor tissues was determined by immunohistochemical staining.

Results: (68)Ga-NOTA-NFB was safe and well tolerated by all subjects. A rapid activity clearance from the blood circulation was observed. The organs with the highest absorbed doses were spleen (193.8 ± 32.5 μSv/MBq) and liver (119.3 ± 25.0 μSv/MBq). The mean effective dose was 25.4 ± 6.1 μSv/MBq. The maximum standardized uptake values (SUVmax) and the maximum target to non-target ratios (T/NTmax) of (68)Ga-NOTA-NFB PET/CT in glioma tissues were 4.11 ± 2.90 (range, 0.45-8.21) and 9.21 ± 8.75 (range, 3.66-24.88), respectively, while those of (18)F-FDG PET/CT were 7.34 ± 2.90 (range, 3.50-12.27) and 0.86 ± 0.41 (range, 0.35-1.59). The histopathological staining confirmed that CXCR4 was overexpressed on resected tumor tissues with prominent (68)Ga-NOTA-NFB uptake.

Conclusion: With a favorable radiation dosimetry profile, (68)Ga-NOTA-NFB is safe for clinical imaging. Compared to (18)F-FDG PET/CT, (68)Ga-NOTA-NFB PET/CT is more sensitive in detecting glioma and could have potential in diagnosing and treatment planning for CXCR4 positive patients.

No MeSH data available.


Related in: MedlinePlus

Hematoxylin-eosin (H&E) and immunohistochemical stains of glioma samples. Upper row (A, C & E): hematoxylin-eosin stains, magnification 400×; lower row (B, D & F): immunohistochemical stains of CXCR4, magnification 400×. The first column (A & B): F, 30 y, grade II; the second column (C & D): M, 61 y, grade III; the third column (E & F): F, 60 y, grade IV.
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Figure 4: Hematoxylin-eosin (H&E) and immunohistochemical stains of glioma samples. Upper row (A, C & E): hematoxylin-eosin stains, magnification 400×; lower row (B, D & F): immunohistochemical stains of CXCR4, magnification 400×. The first column (A & B): F, 30 y, grade II; the second column (C & D): M, 61 y, grade III; the third column (E & F): F, 60 y, grade IV.

Mentions: Hematoxylin-eosin (H&E) and the immunohistochemical staining for CXCR4 were performed in glioma tissues from all 8 patients. Two tumors were graded as WHO II, 1 tumor as WHO III and 5 tumors as WHO IV respectively, according to the H&E staining. For all 8 tumors, the expression of CXCR4 in the resected glioma tissues was confirmed to be positive. The expression of CXCR4 varies from different pathological grades, with low expression for grade II, and high for grade III-IV (Fig. 4), which corroborates with the SUVmax of 68Ga-NOTA-NFB PET.


Prospective Study of (68)Ga-NOTA-NFB: Radiation Dosimetry in Healthy Volunteers and First Application in Glioma Patients.

Wang Z, Zhang M, Wang L, Wang S, Kang F, Li G, Jacobson O, Niu G, Yang W, Wang J, Chen X - Theranostics (2015)

Hematoxylin-eosin (H&E) and immunohistochemical stains of glioma samples. Upper row (A, C & E): hematoxylin-eosin stains, magnification 400×; lower row (B, D & F): immunohistochemical stains of CXCR4, magnification 400×. The first column (A & B): F, 30 y, grade II; the second column (C & D): M, 61 y, grade III; the third column (E & F): F, 60 y, grade IV.
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4440444&req=5

Figure 4: Hematoxylin-eosin (H&E) and immunohistochemical stains of glioma samples. Upper row (A, C & E): hematoxylin-eosin stains, magnification 400×; lower row (B, D & F): immunohistochemical stains of CXCR4, magnification 400×. The first column (A & B): F, 30 y, grade II; the second column (C & D): M, 61 y, grade III; the third column (E & F): F, 60 y, grade IV.
Mentions: Hematoxylin-eosin (H&E) and the immunohistochemical staining for CXCR4 were performed in glioma tissues from all 8 patients. Two tumors were graded as WHO II, 1 tumor as WHO III and 5 tumors as WHO IV respectively, according to the H&E staining. For all 8 tumors, the expression of CXCR4 in the resected glioma tissues was confirmed to be positive. The expression of CXCR4 varies from different pathological grades, with low expression for grade II, and high for grade III-IV (Fig. 4), which corroborates with the SUVmax of 68Ga-NOTA-NFB PET.

Bottom Line: The expression of CXCR4 on the resected brain tumor tissues was determined by immunohistochemical staining. (68)Ga-NOTA-NFB was safe and well tolerated by all subjects.The mean effective dose was 25.4 ± 6.1 μSv/MBq.The histopathological staining confirmed that CXCR4 was overexpressed on resected tumor tissues with prominent (68)Ga-NOTA-NFB uptake.

View Article: PubMed Central - PubMed

Affiliation: 1. Department of Nuclear Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an, China.

ABSTRACT

Purpose: The chemokine receptor CXCR4 is overexpressed in various types of human cancers. As a specific imaging agent of CXCR4, (68)Ga-NOTA-NFB was investigated in this study to assess its safety, biodistribution and dosimetry properties in healthy volunteers, and to preliminarily evaluate its application in glioma patients.

Methods: Six healthy volunteers underwent whole-body PET scans at 0, 0.5, 1, 2 and 3 h after (68)Ga-NOTA-NFB injection (mean dose, 182.4 ± 3.7 MBq (4.93 ± 0.10 mCi)). For time-activity curve calculations, 1 mL blood samples were obtained at 1, 3, 5, 10, 30, 60, 90, 120, 150 and 180 min after the injection. The estimated radiation doses were calculated by OLINDA/EXM software. Eight patients with glioma were enrolled and underwent both (68)Ga-NOTA-NFB and (18)F-FDG PET/CT scans before surgery. The expression of CXCR4 on the resected brain tumor tissues was determined by immunohistochemical staining.

Results: (68)Ga-NOTA-NFB was safe and well tolerated by all subjects. A rapid activity clearance from the blood circulation was observed. The organs with the highest absorbed doses were spleen (193.8 ± 32.5 μSv/MBq) and liver (119.3 ± 25.0 μSv/MBq). The mean effective dose was 25.4 ± 6.1 μSv/MBq. The maximum standardized uptake values (SUVmax) and the maximum target to non-target ratios (T/NTmax) of (68)Ga-NOTA-NFB PET/CT in glioma tissues were 4.11 ± 2.90 (range, 0.45-8.21) and 9.21 ± 8.75 (range, 3.66-24.88), respectively, while those of (18)F-FDG PET/CT were 7.34 ± 2.90 (range, 3.50-12.27) and 0.86 ± 0.41 (range, 0.35-1.59). The histopathological staining confirmed that CXCR4 was overexpressed on resected tumor tissues with prominent (68)Ga-NOTA-NFB uptake.

Conclusion: With a favorable radiation dosimetry profile, (68)Ga-NOTA-NFB is safe for clinical imaging. Compared to (18)F-FDG PET/CT, (68)Ga-NOTA-NFB PET/CT is more sensitive in detecting glioma and could have potential in diagnosing and treatment planning for CXCR4 positive patients.

No MeSH data available.


Related in: MedlinePlus