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Enhanced pharmacological activity of vitamin B₁₂ and penicillin as nanoparticles.

Yariv I, Lipovsky A, Gedanken A, Lubart R, Fixler D - Int J Nanomedicine (2015)

Bottom Line: The sizes of Penicillin and Vitamin B12 nanoparticles (NPs) were found to be 70 and 120-180 nm, respectively.The bactericidal effect of nano Penicillin was studied and found to be higher than that of the bulk form.Reducing the size of Vitamin B12 resulted in their enhanced antioxidative activity as observed using the electron paramagnetic spectroscopy technique.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Engineering and the Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Ramat-Gan, Israel.

ABSTRACT
Sonochemistry has become a well-known technique for fabricating nanomaterials. Since one of the advantages of nanomaterials is that they have higher chemical activities compared with particles in the bulk form, efforts are being made to produce nano organic compounds with enhanced biological activities that could be exploited in the medical area. This study uses the sonication technique to prepare nano Vitamin B12 and nano Penicillin, and demonstrates their enhanced biological and pharmacological activity. The size and morphology of the nano Penicillin and nano Vitamin B12 were investigated using electron microscopy as well as dynamic light scattering techniques. The sizes of Penicillin and Vitamin B12 nanoparticles (NPs) were found to be 70 and 120-180 nm, respectively. The bactericidal effect of nano Penicillin was studied and found to be higher than that of the bulk form. Reducing the size of Vitamin B12 resulted in their enhanced antioxidative activity as observed using the electron paramagnetic spectroscopy technique. The penetration depth of these organic NPs can be detected by an optical iterative method. It is believed that nano organic drugs fabrication will have a great impact on the medical field.

No MeSH data available.


Related in: MedlinePlus

TEM images of Penicillin before and after the ultrasonic radiation.Notes: (A) TEM image of Penicillin bulk form, (B) TEM image of Penicillin NPs after 5-minute sonication, (C) TEM images of Penicillin NPs after 10-minute sonication, (D) an enlargement of TEM image of Penicillin NPs after 10-minute sonication, and (E) diffraction lines of individual Penicillin nanocrystal.Abbreviations: TEM, transmission electron microscopy; NPs, nanoparticles.
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f2-ijn-10-3593: TEM images of Penicillin before and after the ultrasonic radiation.Notes: (A) TEM image of Penicillin bulk form, (B) TEM image of Penicillin NPs after 5-minute sonication, (C) TEM images of Penicillin NPs after 10-minute sonication, (D) an enlargement of TEM image of Penicillin NPs after 10-minute sonication, and (E) diffraction lines of individual Penicillin nanocrystal.Abbreviations: TEM, transmission electron microscopy; NPs, nanoparticles.

Mentions: The morphology and size of both unmodified and nano Penicillin were studied using transmission microscopy analysis. The results of the TEM measurements are shown in (Figure 2). The bulk Penicillin (before the sonochemical reaction) created a thin layer of the compound that virtually covered the entire grid area (Figure 2A). Following the application of ultrasound (US), the formation of Penicillin NPs can be observed clearly in Figure 2B–D. It is also observed that the sonication time had an effect on particle size, namely, as the sonication time increases, the particle size decreases. After 5 minutes of sonication, a clear formation of particles is observed, with larger aggregates of Penicillin still present (Figure 2B). After 10 minutes of sonication (Figure 2C), the amount of particles increased significantly, and their size decreased (Figure 2C). An insert (Figure 2D) shows higher magnification of Penicillin NPs obtained after 10 minutes of sonication. As shown in the figure, the most prominent property of the particles is their uniformity and almost perfect spherical shape; the size of the NPs was measured to be ~70 nm.


Enhanced pharmacological activity of vitamin B₁₂ and penicillin as nanoparticles.

Yariv I, Lipovsky A, Gedanken A, Lubart R, Fixler D - Int J Nanomedicine (2015)

TEM images of Penicillin before and after the ultrasonic radiation.Notes: (A) TEM image of Penicillin bulk form, (B) TEM image of Penicillin NPs after 5-minute sonication, (C) TEM images of Penicillin NPs after 10-minute sonication, (D) an enlargement of TEM image of Penicillin NPs after 10-minute sonication, and (E) diffraction lines of individual Penicillin nanocrystal.Abbreviations: TEM, transmission electron microscopy; NPs, nanoparticles.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4440431&req=5

f2-ijn-10-3593: TEM images of Penicillin before and after the ultrasonic radiation.Notes: (A) TEM image of Penicillin bulk form, (B) TEM image of Penicillin NPs after 5-minute sonication, (C) TEM images of Penicillin NPs after 10-minute sonication, (D) an enlargement of TEM image of Penicillin NPs after 10-minute sonication, and (E) diffraction lines of individual Penicillin nanocrystal.Abbreviations: TEM, transmission electron microscopy; NPs, nanoparticles.
Mentions: The morphology and size of both unmodified and nano Penicillin were studied using transmission microscopy analysis. The results of the TEM measurements are shown in (Figure 2). The bulk Penicillin (before the sonochemical reaction) created a thin layer of the compound that virtually covered the entire grid area (Figure 2A). Following the application of ultrasound (US), the formation of Penicillin NPs can be observed clearly in Figure 2B–D. It is also observed that the sonication time had an effect on particle size, namely, as the sonication time increases, the particle size decreases. After 5 minutes of sonication, a clear formation of particles is observed, with larger aggregates of Penicillin still present (Figure 2B). After 10 minutes of sonication (Figure 2C), the amount of particles increased significantly, and their size decreased (Figure 2C). An insert (Figure 2D) shows higher magnification of Penicillin NPs obtained after 10 minutes of sonication. As shown in the figure, the most prominent property of the particles is their uniformity and almost perfect spherical shape; the size of the NPs was measured to be ~70 nm.

Bottom Line: The sizes of Penicillin and Vitamin B12 nanoparticles (NPs) were found to be 70 and 120-180 nm, respectively.The bactericidal effect of nano Penicillin was studied and found to be higher than that of the bulk form.Reducing the size of Vitamin B12 resulted in their enhanced antioxidative activity as observed using the electron paramagnetic spectroscopy technique.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Engineering and the Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Ramat-Gan, Israel.

ABSTRACT
Sonochemistry has become a well-known technique for fabricating nanomaterials. Since one of the advantages of nanomaterials is that they have higher chemical activities compared with particles in the bulk form, efforts are being made to produce nano organic compounds with enhanced biological activities that could be exploited in the medical area. This study uses the sonication technique to prepare nano Vitamin B12 and nano Penicillin, and demonstrates their enhanced biological and pharmacological activity. The size and morphology of the nano Penicillin and nano Vitamin B12 were investigated using electron microscopy as well as dynamic light scattering techniques. The sizes of Penicillin and Vitamin B12 nanoparticles (NPs) were found to be 70 and 120-180 nm, respectively. The bactericidal effect of nano Penicillin was studied and found to be higher than that of the bulk form. Reducing the size of Vitamin B12 resulted in their enhanced antioxidative activity as observed using the electron paramagnetic spectroscopy technique. The penetration depth of these organic NPs can be detected by an optical iterative method. It is believed that nano organic drugs fabrication will have a great impact on the medical field.

No MeSH data available.


Related in: MedlinePlus