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Multicomponent signal unmixing from nanoheterostructures: overcoming the traditional challenges of nanoscale X-ray analysis via machine learning.

Rossouw D, Burdet P, de la Peña F, Ducati C, Knappett BR, Wheatley AE, Midgley PA - Nano Lett. (2015)

Bottom Line: The chemical composition of core-shell nanoparticle clusters have been determined through principal component analysis (PCA) and independent component analysis (ICA) of an energy-dispersive X-ray (EDX) spectrum image (SI) acquired in a scanning transmission electron microscope (STEM).The method blindly decomposes the SI into three components, which are found to accurately represent the isolated and unmixed X-ray signals originating from the supporting carbon film, the shell, and the bimetallic core.The composition of the latter is verified by and is in excellent agreement with the separate quantification of bare bimetallic seed nanoparticles.

View Article: PubMed Central - PubMed

Affiliation: †Department of Materials Science and Metallurgy, University of Cambridge, 27 Charles Babbage Road, Cambridge CB3 0FS, United Kingdom.

ABSTRACT
The chemical composition of core-shell nanoparticle clusters have been determined through principal component analysis (PCA) and independent component analysis (ICA) of an energy-dispersive X-ray (EDX) spectrum image (SI) acquired in a scanning transmission electron microscope (STEM). The method blindly decomposes the SI into three components, which are found to accurately represent the isolated and unmixed X-ray signals originating from the supporting carbon film, the shell, and the bimetallic core. The composition of the latter is verified by and is in excellent agreement with the separate quantification of bare bimetallic seed nanoparticles.

No MeSH data available.


Related in: MedlinePlus

Summary of the composition of 103 bareFePt bimetallic nanoparticlesextracted from a synthesis prior to the shell addition step. (a) Selectedcluster of FePt seed particles. (b) Segmentation of the EDX spectrumimage prior to quantification of each particle. (c) Fitting of theEDX spectrum from a single seed (circled in (b)) to a model spectrumto determine the Fe Kα and Pt Lα peak intensities. (d)Particle seed compositions obtained by quantifying the fitted intensitiesfrom 103 different particles (error bars = 1 standard deviation).
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fig5: Summary of the composition of 103 bareFePt bimetallic nanoparticlesextracted from a synthesis prior to the shell addition step. (a) Selectedcluster of FePt seed particles. (b) Segmentation of the EDX spectrumimage prior to quantification of each particle. (c) Fitting of theEDX spectrum from a single seed (circled in (b)) to a model spectrumto determine the Fe Kα and Pt Lα peak intensities. (d)Particle seed compositions obtained by quantifying the fitted intensitiesfrom 103 different particles (error bars = 1 standard deviation).

Mentions: A total of 12 EDX SIs were acquired in order to capture andanalyzemultiple FePt bimetallic seed clusters. Image segmentation was performedusing thresholding22 or a watershed algorithm23 where appropriate. The segmentation of one ofthe EDX SIs is shown in Figure 5a,b.


Multicomponent signal unmixing from nanoheterostructures: overcoming the traditional challenges of nanoscale X-ray analysis via machine learning.

Rossouw D, Burdet P, de la Peña F, Ducati C, Knappett BR, Wheatley AE, Midgley PA - Nano Lett. (2015)

Summary of the composition of 103 bareFePt bimetallic nanoparticlesextracted from a synthesis prior to the shell addition step. (a) Selectedcluster of FePt seed particles. (b) Segmentation of the EDX spectrumimage prior to quantification of each particle. (c) Fitting of theEDX spectrum from a single seed (circled in (b)) to a model spectrumto determine the Fe Kα and Pt Lα peak intensities. (d)Particle seed compositions obtained by quantifying the fitted intensitiesfrom 103 different particles (error bars = 1 standard deviation).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4440406&req=5

fig5: Summary of the composition of 103 bareFePt bimetallic nanoparticlesextracted from a synthesis prior to the shell addition step. (a) Selectedcluster of FePt seed particles. (b) Segmentation of the EDX spectrumimage prior to quantification of each particle. (c) Fitting of theEDX spectrum from a single seed (circled in (b)) to a model spectrumto determine the Fe Kα and Pt Lα peak intensities. (d)Particle seed compositions obtained by quantifying the fitted intensitiesfrom 103 different particles (error bars = 1 standard deviation).
Mentions: A total of 12 EDX SIs were acquired in order to capture andanalyzemultiple FePt bimetallic seed clusters. Image segmentation was performedusing thresholding22 or a watershed algorithm23 where appropriate. The segmentation of one ofthe EDX SIs is shown in Figure 5a,b.

Bottom Line: The chemical composition of core-shell nanoparticle clusters have been determined through principal component analysis (PCA) and independent component analysis (ICA) of an energy-dispersive X-ray (EDX) spectrum image (SI) acquired in a scanning transmission electron microscope (STEM).The method blindly decomposes the SI into three components, which are found to accurately represent the isolated and unmixed X-ray signals originating from the supporting carbon film, the shell, and the bimetallic core.The composition of the latter is verified by and is in excellent agreement with the separate quantification of bare bimetallic seed nanoparticles.

View Article: PubMed Central - PubMed

Affiliation: †Department of Materials Science and Metallurgy, University of Cambridge, 27 Charles Babbage Road, Cambridge CB3 0FS, United Kingdom.

ABSTRACT
The chemical composition of core-shell nanoparticle clusters have been determined through principal component analysis (PCA) and independent component analysis (ICA) of an energy-dispersive X-ray (EDX) spectrum image (SI) acquired in a scanning transmission electron microscope (STEM). The method blindly decomposes the SI into three components, which are found to accurately represent the isolated and unmixed X-ray signals originating from the supporting carbon film, the shell, and the bimetallic core. The composition of the latter is verified by and is in excellent agreement with the separate quantification of bare bimetallic seed nanoparticles.

No MeSH data available.


Related in: MedlinePlus