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Comprehensive confocal endomicroscopy of the esophagus in vivo.

Kang D, Schlachter SC, Carruth RW, Kim M, Wu T, Tabatabaei N, Vacas-Jacques P, Shishkov M, Woods K, Sauk JS, Leung J, Nishioka NS, Tearney GJ - Endosc Int Open (2014)

Bottom Line: Very large confocal microscopy images (length = 5 cm; circumference = 2.2 cm) of swine esophagus from three imaging depths, spanning a total area of 33 cm(2), were obtained in about 2 minutes.SECM images enabled the visualization of cellular morphology of the swine esophagus, including stratified squamous cell nuclei, basal cells, and collagen within the lamina propria.The results from this study suggest that the SECM technology can rapidly provide large, contiguous confocal microscopy images of the esophagus in vivo.

View Article: PubMed Central - PubMed

Affiliation: Massachusetts General Hospital - Wellman Center for Photomedicine, Boston, MA.

ABSTRACT

Background and study aims: Biopsy sampling error can be a problem for the diagnosis of certain gastrointestinal tract diseases. Spectrally-encoded confocal microscopy (SECM) is a high-speed reflectance confocal microscopy technology that has the potential to overcome sampling error by imaging large regions of gastrointestinal tract tissues. The aim of this study was to test a recently developed SECM endoscopic probe for comprehensively imaging large segments of the esophagus at the microscopic level in vivo.

Methods: Topical acetic acid was endoscopically applied to the esophagus of a normal living swine. The 7 mm diameter SECM endoscopic probe was transorally introduced into the esophagus over a wire. Optics within the SECM probe were helically scanned over a 5 cm length of the esophagus. Confocal microscopy data was displayed and stored in real time.

Results: Very large confocal microscopy images (length = 5 cm; circumference = 2.2 cm) of swine esophagus from three imaging depths, spanning a total area of 33 cm(2), were obtained in about 2 minutes. SECM images enabled the visualization of cellular morphology of the swine esophagus, including stratified squamous cell nuclei, basal cells, and collagen within the lamina propria.

Conclusions: The results from this study suggest that the SECM technology can rapidly provide large, contiguous confocal microscopy images of the esophagus in vivo. When applied to human subjects, the unique comprehensive, microscopic imaging capabilities of this technology may be utilized for improving the screening and surveillance of various esophageal diseases.

No MeSH data available.


Related in: MedlinePlus

 High-magnification SECM images (a, b, c) and representative, nearby en face histologic images (d, e, f) of swine esophagus. SECM images were taken from the regions marked by the boxes 1, 2, and 3 in Fig. 2 a. a and d Stratified squamous epithelium; b and e basal cell layer; c and f lamina propria (scale bar = 100 µm).
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FI047-3:  High-magnification SECM images (a, b, c) and representative, nearby en face histologic images (d, e, f) of swine esophagus. SECM images were taken from the regions marked by the boxes 1, 2, and 3 in Fig. 2 a. a and d Stratified squamous epithelium; b and e basal cell layer; c and f lamina propria (scale bar = 100 µm).

Mentions: Cellular features were visualized in SECM images at various depths within the epithelium. Fig. 3 shows high-magnification SECM images at three distinctive locations and en face histologic images of the swine esophagus. Fig. 3 a, a SECM image taken from a superficial plane within the epithelium (box 1 in Fig. 2 a), shows scattered nuclei (bright dots) similar to that seen in a histologic image of representative, nearby superficial squamous epithelium (Fig. 3 d). Fig. 3 b, a SECM image taken from a deeper region of the epithelium (box 2 in Fig. 2 a), demonstrates numerous nuclei (bright dots) with a higher density than Fig. 3 a and multiple papillae (dark circular regions) surrounded by the nuclei. Similar cellular features are observed in a representative histologic image of the basal cell layer (Fig. 3 e). Fig. 3 c, an SECM region from the LP (box 3 in Fig. 2 a), enables the visualization of microstructures with high SECM signals that are similar in appearance to collagen seen in a representative histologic image of the LP (Fig. 3 f).


Comprehensive confocal endomicroscopy of the esophagus in vivo.

Kang D, Schlachter SC, Carruth RW, Kim M, Wu T, Tabatabaei N, Vacas-Jacques P, Shishkov M, Woods K, Sauk JS, Leung J, Nishioka NS, Tearney GJ - Endosc Int Open (2014)

 High-magnification SECM images (a, b, c) and representative, nearby en face histologic images (d, e, f) of swine esophagus. SECM images were taken from the regions marked by the boxes 1, 2, and 3 in Fig. 2 a. a and d Stratified squamous epithelium; b and e basal cell layer; c and f lamina propria (scale bar = 100 µm).
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4440396&req=5

FI047-3:  High-magnification SECM images (a, b, c) and representative, nearby en face histologic images (d, e, f) of swine esophagus. SECM images were taken from the regions marked by the boxes 1, 2, and 3 in Fig. 2 a. a and d Stratified squamous epithelium; b and e basal cell layer; c and f lamina propria (scale bar = 100 µm).
Mentions: Cellular features were visualized in SECM images at various depths within the epithelium. Fig. 3 shows high-magnification SECM images at three distinctive locations and en face histologic images of the swine esophagus. Fig. 3 a, a SECM image taken from a superficial plane within the epithelium (box 1 in Fig. 2 a), shows scattered nuclei (bright dots) similar to that seen in a histologic image of representative, nearby superficial squamous epithelium (Fig. 3 d). Fig. 3 b, a SECM image taken from a deeper region of the epithelium (box 2 in Fig. 2 a), demonstrates numerous nuclei (bright dots) with a higher density than Fig. 3 a and multiple papillae (dark circular regions) surrounded by the nuclei. Similar cellular features are observed in a representative histologic image of the basal cell layer (Fig. 3 e). Fig. 3 c, an SECM region from the LP (box 3 in Fig. 2 a), enables the visualization of microstructures with high SECM signals that are similar in appearance to collagen seen in a representative histologic image of the LP (Fig. 3 f).

Bottom Line: Very large confocal microscopy images (length = 5 cm; circumference = 2.2 cm) of swine esophagus from three imaging depths, spanning a total area of 33 cm(2), were obtained in about 2 minutes.SECM images enabled the visualization of cellular morphology of the swine esophagus, including stratified squamous cell nuclei, basal cells, and collagen within the lamina propria.The results from this study suggest that the SECM technology can rapidly provide large, contiguous confocal microscopy images of the esophagus in vivo.

View Article: PubMed Central - PubMed

Affiliation: Massachusetts General Hospital - Wellman Center for Photomedicine, Boston, MA.

ABSTRACT

Background and study aims: Biopsy sampling error can be a problem for the diagnosis of certain gastrointestinal tract diseases. Spectrally-encoded confocal microscopy (SECM) is a high-speed reflectance confocal microscopy technology that has the potential to overcome sampling error by imaging large regions of gastrointestinal tract tissues. The aim of this study was to test a recently developed SECM endoscopic probe for comprehensively imaging large segments of the esophagus at the microscopic level in vivo.

Methods: Topical acetic acid was endoscopically applied to the esophagus of a normal living swine. The 7 mm diameter SECM endoscopic probe was transorally introduced into the esophagus over a wire. Optics within the SECM probe were helically scanned over a 5 cm length of the esophagus. Confocal microscopy data was displayed and stored in real time.

Results: Very large confocal microscopy images (length = 5 cm; circumference = 2.2 cm) of swine esophagus from three imaging depths, spanning a total area of 33 cm(2), were obtained in about 2 minutes. SECM images enabled the visualization of cellular morphology of the swine esophagus, including stratified squamous cell nuclei, basal cells, and collagen within the lamina propria.

Conclusions: The results from this study suggest that the SECM technology can rapidly provide large, contiguous confocal microscopy images of the esophagus in vivo. When applied to human subjects, the unique comprehensive, microscopic imaging capabilities of this technology may be utilized for improving the screening and surveillance of various esophageal diseases.

No MeSH data available.


Related in: MedlinePlus