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Clinical and laboratory studies of the fate of intranasal allergen.

Rimmer J, Santos C, Yli-Panula E, Noronha V, Viander M - PLoS ONE (2015)

Bottom Line: The precise way in which allergen is handled by the nose is unknown.The median of carbon particles recovered was 9%. (2) Prechallenge Der p 1 staining was associated with the epithelium and subepithelial mucous glands.After challenge the nose effectively retains allergen, which remains mucosally associated; in atopics there is greater Der p 1 deposition and inflammatory response than in nonatopics.

View Article: PubMed Central - PubMed

Affiliation: Allergen Group, Woolcock Institute of Medical Research, Sydney, New South Wales, Australia; Sydney Medical School, the University of Sydney, Sydney, New South Wales, Australia.

ABSTRACT

Background: The precise way in which allergen is handled by the nose is unknown. The objective of this study was to determine recovery of Der p 1 allergen following nasal administration and to determine whether Der p 1 can be detected in nasal biopsies after natural exposure and nasal challenge to allergen.

Methods: (1) 20 nonatopic non-rhinitics were challenged with Der p 1 and recovery was measured by ELISA in the nasal wash, nasal mucus and induced sputum up to 30 minutes. Particulate charcoal (<40 μm) served as control. (2) In 8 subjects (5 atopics), 30 to 60 minutes after challenge histological localisation of Der p 1 in the nasal mucosal epithelium, subepithelial mucous glands and lamina propria was performed. Co-localisation of Der p 1 with macrophages and IgE-positive cells was undertaken.

Results: (1) Less than 25% of total allergen was retrievable after aqueous or particulate challenge, most from the nasal mucus during 1-5 min after the challenge. The median of carbon particles recovered was 9%. (2) Prechallenge Der p 1 staining was associated with the epithelium and subepithelial mucous glands. After challenge there was a trend for greater Der p 1 deposition in atopics, but both atopics and nonatopics showed increases in the number of Der p 1 stained cells and stained tissue compartments. In atopics, increased eosinophils, macrophages and IgE positive cells co-localized with Der p 1 staining.

Conclusions: Der p 1 allergen is detected in nasal tissue independent of atopic status after natural exposure. After challenge the nose effectively retains allergen, which remains mucosally associated; in atopics there is greater Der p 1 deposition and inflammatory response than in nonatopics. These results support the hypothesis that nasal mucus and tissue act as a reservoir for the inhaled Der p 1 allergen leading to a persistent allergic inflammatory response in susceptible individuals.

No MeSH data available.


Related in: MedlinePlus

Percent recovery of allergen from different compartments.
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pone.0127477.g001: Percent recovery of allergen from different compartments.

Mentions: The mite allergen recovered from each compartment: nasal wash, nasal mucus, gargle and sputum samples after an aqueous or particulate mite allergen nasal challenge, is presented in Fig 1. The amount of allergen recovered from the nose at times 1, 5 and 10 minutes after nasal aqueous challenge was highest in the nasal mucus compared with the nasal wash or lower respiratory tract samples (gargle, induced sputum; p = 0.04, p = 0.01, p = 0.02, respectively; Friedman test for multiple samples). A significantly higher amount of allergen was detected in nasal mucus compared to nasal wash at 5 min after aqueous challenge (Fig 1A; p = 0.03; Wilcoxon signed rank test). When all the different compartments and sampling times were compared the highest percent of allergen recovery (median = 12.0%, IQR = 4.4) was into the nasal mucus compartment five minutes after the nasal aqueous allergen challenge (p = 0.01) (Fig 1A). When combined allergen recovery from the upper respiratory tract (nasal wash and nasal mucus) was compared with sampling from the oropharynx and lower respiratory tract (gargle and induced sputum) much greater amounts of allergen were obtained from the upper airway or nose for both aqueous (p<0.001) and particulate (p<0.001) challenges (analysis of variance, Friedman test for multiple samples).


Clinical and laboratory studies of the fate of intranasal allergen.

Rimmer J, Santos C, Yli-Panula E, Noronha V, Viander M - PLoS ONE (2015)

Percent recovery of allergen from different compartments.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4430540&req=5

pone.0127477.g001: Percent recovery of allergen from different compartments.
Mentions: The mite allergen recovered from each compartment: nasal wash, nasal mucus, gargle and sputum samples after an aqueous or particulate mite allergen nasal challenge, is presented in Fig 1. The amount of allergen recovered from the nose at times 1, 5 and 10 minutes after nasal aqueous challenge was highest in the nasal mucus compared with the nasal wash or lower respiratory tract samples (gargle, induced sputum; p = 0.04, p = 0.01, p = 0.02, respectively; Friedman test for multiple samples). A significantly higher amount of allergen was detected in nasal mucus compared to nasal wash at 5 min after aqueous challenge (Fig 1A; p = 0.03; Wilcoxon signed rank test). When all the different compartments and sampling times were compared the highest percent of allergen recovery (median = 12.0%, IQR = 4.4) was into the nasal mucus compartment five minutes after the nasal aqueous allergen challenge (p = 0.01) (Fig 1A). When combined allergen recovery from the upper respiratory tract (nasal wash and nasal mucus) was compared with sampling from the oropharynx and lower respiratory tract (gargle and induced sputum) much greater amounts of allergen were obtained from the upper airway or nose for both aqueous (p<0.001) and particulate (p<0.001) challenges (analysis of variance, Friedman test for multiple samples).

Bottom Line: The precise way in which allergen is handled by the nose is unknown.The median of carbon particles recovered was 9%. (2) Prechallenge Der p 1 staining was associated with the epithelium and subepithelial mucous glands.After challenge the nose effectively retains allergen, which remains mucosally associated; in atopics there is greater Der p 1 deposition and inflammatory response than in nonatopics.

View Article: PubMed Central - PubMed

Affiliation: Allergen Group, Woolcock Institute of Medical Research, Sydney, New South Wales, Australia; Sydney Medical School, the University of Sydney, Sydney, New South Wales, Australia.

ABSTRACT

Background: The precise way in which allergen is handled by the nose is unknown. The objective of this study was to determine recovery of Der p 1 allergen following nasal administration and to determine whether Der p 1 can be detected in nasal biopsies after natural exposure and nasal challenge to allergen.

Methods: (1) 20 nonatopic non-rhinitics were challenged with Der p 1 and recovery was measured by ELISA in the nasal wash, nasal mucus and induced sputum up to 30 minutes. Particulate charcoal (<40 μm) served as control. (2) In 8 subjects (5 atopics), 30 to 60 minutes after challenge histological localisation of Der p 1 in the nasal mucosal epithelium, subepithelial mucous glands and lamina propria was performed. Co-localisation of Der p 1 with macrophages and IgE-positive cells was undertaken.

Results: (1) Less than 25% of total allergen was retrievable after aqueous or particulate challenge, most from the nasal mucus during 1-5 min after the challenge. The median of carbon particles recovered was 9%. (2) Prechallenge Der p 1 staining was associated with the epithelium and subepithelial mucous glands. After challenge there was a trend for greater Der p 1 deposition in atopics, but both atopics and nonatopics showed increases in the number of Der p 1 stained cells and stained tissue compartments. In atopics, increased eosinophils, macrophages and IgE positive cells co-localized with Der p 1 staining.

Conclusions: Der p 1 allergen is detected in nasal tissue independent of atopic status after natural exposure. After challenge the nose effectively retains allergen, which remains mucosally associated; in atopics there is greater Der p 1 deposition and inflammatory response than in nonatopics. These results support the hypothesis that nasal mucus and tissue act as a reservoir for the inhaled Der p 1 allergen leading to a persistent allergic inflammatory response in susceptible individuals.

No MeSH data available.


Related in: MedlinePlus