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Different Dynamic Patterns of β-Lactams, Quinolones, Glycopeptides and Macrolides on Mouse Gut Microbial Diversity.

Yin J, M P, Wang S, Liao SX, Peng X, He Y, Chen YR, Shen HF, Su J, Chen Y, Jiang YX, Zhang GX, Zhou HW - PLoS ONE (2015)

Bottom Line: In general, the community changes induced by the three β-lactam antibiotics showed consistency in inhibiting Papillibacter, Prevotella and Alistipes while inducing massive growth of Clostridium.Vancomycin was unique in that it inhibited Firmicutes, mainly the genus Clostridium.Azithromycin and meropenem induced the growth of Enterococcus.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, Nanfang Hospital, Southern Medical University, Guangzhou, China 510515.

ABSTRACT
The adverse impact of antibiotics on the gut microbiota has attracted extensive interest, particularly due to the development of microbiome research techniques in recent years. However, a direct comparison of the dynamic effects of various types of antibiotics using the same animal model has not been available. In the present study, we selected six antibiotics from four categories with the broadest clinical usage, namely, β-lactams (Ceftriaxone Sodium, Cefoperazone/Sulbactam and meropenem), quinolones (ofloxacin), glycopeptides (vancomycin), and macrolides (azithromycin), to treat BALB/c mice. Stool samples were collected during and after the administration of antibiotics, and microbial diversity was analyzed through Illumina sequencing and bioinformatics analyses using QIIME. Both α and β diversity analyses showed that ceftriaxone sodium, cefoperazone/sulbactam, meropenem and vancomycin changed the gut microbiota dramatically by the second day of antibiotic administration whereas the influence of ofloxacin was trivial. Azithromycin clearly changed the gut microbiota but much less than vancomycin and the β-lactams. In general, the community changes induced by the three β-lactam antibiotics showed consistency in inhibiting Papillibacter, Prevotella and Alistipes while inducing massive growth of Clostridium. The low diversity and high Clostridium level might be an important cause of Clostridium difficile infection after usage of β-lactams. Vancomycin was unique in that it inhibited Firmicutes, mainly the genus Clostridium. On the other hand, it induced the growth of Escherichia and effect lasted for months afterward. Azithromycin and meropenem induced the growth of Enterococcus. These findings will be useful for understanding the potential adverse effects of antibiotics on the gut microbiome and ensuring their better usage.

No MeSH data available.


Related in: MedlinePlus

Principal coordinates analysis of unweighted and weighted UniFrac distances among different antibiotic groups.The starting points of the arrow lines are from the second day of antibiotic administration, and the arrow point is the end of observation period.
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pone.0126712.g002: Principal coordinates analysis of unweighted and weighted UniFrac distances among different antibiotic groups.The starting points of the arrow lines are from the second day of antibiotic administration, and the arrow point is the end of observation period.

Mentions: We additionally studied the PCoA of UniFrac distance among different antibiotic groups (Fig 2). We regard the position of drug delivery as the starting point and the end of the observation period as the terminal point. Each point represents a whole bacterial community, and the points far from each other represent lower similarity between the two communities. The weighted and unweighted UniFrac results of the OFL group did not indicate any changes, which is consistent with the UniFrac results that were affected by time. The influence of AZI was mild and was similar to the control group. The weighted UniFrac results clearly showed that the trajectories of the CTR, CPZ and MEC groups were similar, while the VAN group changed in accordance with the weighted UniFrac results of the VAN group (S2 Fig).


Different Dynamic Patterns of β-Lactams, Quinolones, Glycopeptides and Macrolides on Mouse Gut Microbial Diversity.

Yin J, M P, Wang S, Liao SX, Peng X, He Y, Chen YR, Shen HF, Su J, Chen Y, Jiang YX, Zhang GX, Zhou HW - PLoS ONE (2015)

Principal coordinates analysis of unweighted and weighted UniFrac distances among different antibiotic groups.The starting points of the arrow lines are from the second day of antibiotic administration, and the arrow point is the end of observation period.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4430517&req=5

pone.0126712.g002: Principal coordinates analysis of unweighted and weighted UniFrac distances among different antibiotic groups.The starting points of the arrow lines are from the second day of antibiotic administration, and the arrow point is the end of observation period.
Mentions: We additionally studied the PCoA of UniFrac distance among different antibiotic groups (Fig 2). We regard the position of drug delivery as the starting point and the end of the observation period as the terminal point. Each point represents a whole bacterial community, and the points far from each other represent lower similarity between the two communities. The weighted and unweighted UniFrac results of the OFL group did not indicate any changes, which is consistent with the UniFrac results that were affected by time. The influence of AZI was mild and was similar to the control group. The weighted UniFrac results clearly showed that the trajectories of the CTR, CPZ and MEC groups were similar, while the VAN group changed in accordance with the weighted UniFrac results of the VAN group (S2 Fig).

Bottom Line: In general, the community changes induced by the three β-lactam antibiotics showed consistency in inhibiting Papillibacter, Prevotella and Alistipes while inducing massive growth of Clostridium.Vancomycin was unique in that it inhibited Firmicutes, mainly the genus Clostridium.Azithromycin and meropenem induced the growth of Enterococcus.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, Nanfang Hospital, Southern Medical University, Guangzhou, China 510515.

ABSTRACT
The adverse impact of antibiotics on the gut microbiota has attracted extensive interest, particularly due to the development of microbiome research techniques in recent years. However, a direct comparison of the dynamic effects of various types of antibiotics using the same animal model has not been available. In the present study, we selected six antibiotics from four categories with the broadest clinical usage, namely, β-lactams (Ceftriaxone Sodium, Cefoperazone/Sulbactam and meropenem), quinolones (ofloxacin), glycopeptides (vancomycin), and macrolides (azithromycin), to treat BALB/c mice. Stool samples were collected during and after the administration of antibiotics, and microbial diversity was analyzed through Illumina sequencing and bioinformatics analyses using QIIME. Both α and β diversity analyses showed that ceftriaxone sodium, cefoperazone/sulbactam, meropenem and vancomycin changed the gut microbiota dramatically by the second day of antibiotic administration whereas the influence of ofloxacin was trivial. Azithromycin clearly changed the gut microbiota but much less than vancomycin and the β-lactams. In general, the community changes induced by the three β-lactam antibiotics showed consistency in inhibiting Papillibacter, Prevotella and Alistipes while inducing massive growth of Clostridium. The low diversity and high Clostridium level might be an important cause of Clostridium difficile infection after usage of β-lactams. Vancomycin was unique in that it inhibited Firmicutes, mainly the genus Clostridium. On the other hand, it induced the growth of Escherichia and effect lasted for months afterward. Azithromycin and meropenem induced the growth of Enterococcus. These findings will be useful for understanding the potential adverse effects of antibiotics on the gut microbiome and ensuring their better usage.

No MeSH data available.


Related in: MedlinePlus