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Mapping a Large Number of QTL for Durable Resistance to Stripe Rust in Winter Wheat Druchamp Using SSR and SNP Markers.

Hou L, Chen X, Wang M, See DR, Chao S, Bulli P, Jing J - PLoS ONE (2015)

Bottom Line: Among the eight HTAP resistance QTL, QYrdr.wgp-1BL.2 (explaining 2.36-31.04% variation), QYrdr.wgp-2BL (2.81-15.65%), QYrdr.wgp-5AL (2.27-17.22%) and QYrdr.wgp-5BL.2 (2.42-15.13%) were significant in all tests; and QYrdr.wgp-1BL.1 (1.94-10.19%), QYrdr.wgp-1DS (2.04-27.24%), QYrdr.wgp-3AL (1.78-13.85%) and QYrdr.wgp-6BL.2 (1.69-33.71%) were significant in some of the tests.The three all-stage resistance QTL, QYrdr.wgp-5BL.1 (5.47-36.04%), QYrdr.wgp-5DL (9.27-11.94%) and QYrdr.wgp-6BL.1 (13.07-20.36%), were detected based on reactions in the seedlings tested with certain Pst races.Among the eleven QTL detected in Druchamp, at least three (QYrdr.wgp-5DL for race-specific all-stage resistance and QYrdr.wgp-3AL and QYrdr.wgp-6BL.2 for race non-specific HTAP resistance) are new.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Agricultural Integrated Pest Management, Institute of Plant Protection, Qinghai Academy of Agriculture and Forestry Sciences, Xining, Qinghai, China; Department of Plant Pathology, Washington State University, Pullman, Washington, United States of America; State Key Laboratory of Crop Stress Biology in Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, Shaanxi, China.

ABSTRACT
Winter wheat Druchamp has both high-temperature adult-plant (HTAP) resistance and all-stage resistance to stripe rust caused by Puccinia striiformis f. sp. tritici (Pst). The HTAP resistance in Druchamp is durable as the variety has been resistant in adult-plant stage since it was introduced from France to the United States in late 1940s. To map the quantitative trait loci (QTL) for stripe rust resistance, an F8 recombinant inbred line (RIL) population from cross Druchamp × Michigan Amber was phenotyped for stripe rust response in multiple years in fields under natural infection and with selected Pst races under controlled greenhouse conditions, and genotyped with simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) markers. Composite interval mapping (CIM) identified eight HTAP resistance QTL and three all-stage resistance QTL. Among the eight HTAP resistance QTL, QYrdr.wgp-1BL.2 (explaining 2.36-31.04% variation), QYrdr.wgp-2BL (2.81-15.65%), QYrdr.wgp-5AL (2.27-17.22%) and QYrdr.wgp-5BL.2 (2.42-15.13%) were significant in all tests; and QYrdr.wgp-1BL.1 (1.94-10.19%), QYrdr.wgp-1DS (2.04-27.24%), QYrdr.wgp-3AL (1.78-13.85%) and QYrdr.wgp-6BL.2 (1.69-33.71%) were significant in some of the tests. The three all-stage resistance QTL, QYrdr.wgp-5BL.1 (5.47-36.04%), QYrdr.wgp-5DL (9.27-11.94%) and QYrdr.wgp-6BL.1 (13.07-20.36%), were detected based on reactions in the seedlings tested with certain Pst races. Among the eleven QTL detected in Druchamp, at least three (QYrdr.wgp-5DL for race-specific all-stage resistance and QYrdr.wgp-3AL and QYrdr.wgp-6BL.2 for race non-specific HTAP resistance) are new. All these QTL, especially those for durable HTAP resistance, and their closely linked molecular markers could be useful for developing wheat cultivars with durable resistance to stripe rust.

No MeSH data available.


Related in: MedlinePlus

Linkage maps for high-temperature adult-plant (HTAP) resistance eight QTL identified in the Druchamp × Michigan Amber derived recombinant inbred line population using stripe rust phenotypic data obtained under various environments and genotypic data of simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) markers.A total of eight linkage maps were identified. Fig 4.1 shows QTL QYrdr.wgp-1BL.1, QYrdr.wgp-1BL.2 and QYrdr.wgp-2BL; Fig 4.2 shows QYrdr.wgp-1DS, QYrdr.wgp-3AL, and QYrdr.wgp-5AL; and Fig 4.3 shows QYrdr.wgp-5BL.2 and QYrdr.wgp-6BL.2. Markers with prefix X are SSR markers and those with prefix IWA) are SNPs. The locations of the QTL are indicated by the arrows and the markers in bold.
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pone.0126794.g004: Linkage maps for high-temperature adult-plant (HTAP) resistance eight QTL identified in the Druchamp × Michigan Amber derived recombinant inbred line population using stripe rust phenotypic data obtained under various environments and genotypic data of simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) markers.A total of eight linkage maps were identified. Fig 4.1 shows QTL QYrdr.wgp-1BL.1, QYrdr.wgp-1BL.2 and QYrdr.wgp-2BL; Fig 4.2 shows QYrdr.wgp-1DS, QYrdr.wgp-3AL, and QYrdr.wgp-5AL; and Fig 4.3 shows QYrdr.wgp-5BL.2 and QYrdr.wgp-6BL.2. Markers with prefix X are SSR markers and those with prefix IWA) are SNPs. The locations of the QTL are indicated by the arrows and the markers in bold.

Mentions: Eight QTL for HTAP resistance were detected using the IT and rAUDPC data of the six field experiments, combined mean IT and rAUDPC data of the six field experiments and the two race greenhouse tests. Two of the QTL were mapped on chromosomes 1BL and one each on 1DS, 2BL, 3AL, 5AL, 5BL and 6BL (Fig 4; Tables 5 and 6). These QTL were designated as QYrdr.wgp-1BL.1, QYrdr.wgp-1BL.2, QYrdr.wgp-1DS, QYrdr.wgp-2BL, QYrdr.wgp-3AL, QYrdr.wgp-5AL, QYrdr.wgp-5BL.2 and QYrdr.wgp-6BL.2, respectively. All QTL for either rAUDPC or IT were significant and were contributed by Druchamp.


Mapping a Large Number of QTL for Durable Resistance to Stripe Rust in Winter Wheat Druchamp Using SSR and SNP Markers.

Hou L, Chen X, Wang M, See DR, Chao S, Bulli P, Jing J - PLoS ONE (2015)

Linkage maps for high-temperature adult-plant (HTAP) resistance eight QTL identified in the Druchamp × Michigan Amber derived recombinant inbred line population using stripe rust phenotypic data obtained under various environments and genotypic data of simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) markers.A total of eight linkage maps were identified. Fig 4.1 shows QTL QYrdr.wgp-1BL.1, QYrdr.wgp-1BL.2 and QYrdr.wgp-2BL; Fig 4.2 shows QYrdr.wgp-1DS, QYrdr.wgp-3AL, and QYrdr.wgp-5AL; and Fig 4.3 shows QYrdr.wgp-5BL.2 and QYrdr.wgp-6BL.2. Markers with prefix X are SSR markers and those with prefix IWA) are SNPs. The locations of the QTL are indicated by the arrows and the markers in bold.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4430513&req=5

pone.0126794.g004: Linkage maps for high-temperature adult-plant (HTAP) resistance eight QTL identified in the Druchamp × Michigan Amber derived recombinant inbred line population using stripe rust phenotypic data obtained under various environments and genotypic data of simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) markers.A total of eight linkage maps were identified. Fig 4.1 shows QTL QYrdr.wgp-1BL.1, QYrdr.wgp-1BL.2 and QYrdr.wgp-2BL; Fig 4.2 shows QYrdr.wgp-1DS, QYrdr.wgp-3AL, and QYrdr.wgp-5AL; and Fig 4.3 shows QYrdr.wgp-5BL.2 and QYrdr.wgp-6BL.2. Markers with prefix X are SSR markers and those with prefix IWA) are SNPs. The locations of the QTL are indicated by the arrows and the markers in bold.
Mentions: Eight QTL for HTAP resistance were detected using the IT and rAUDPC data of the six field experiments, combined mean IT and rAUDPC data of the six field experiments and the two race greenhouse tests. Two of the QTL were mapped on chromosomes 1BL and one each on 1DS, 2BL, 3AL, 5AL, 5BL and 6BL (Fig 4; Tables 5 and 6). These QTL were designated as QYrdr.wgp-1BL.1, QYrdr.wgp-1BL.2, QYrdr.wgp-1DS, QYrdr.wgp-2BL, QYrdr.wgp-3AL, QYrdr.wgp-5AL, QYrdr.wgp-5BL.2 and QYrdr.wgp-6BL.2, respectively. All QTL for either rAUDPC or IT were significant and were contributed by Druchamp.

Bottom Line: Among the eight HTAP resistance QTL, QYrdr.wgp-1BL.2 (explaining 2.36-31.04% variation), QYrdr.wgp-2BL (2.81-15.65%), QYrdr.wgp-5AL (2.27-17.22%) and QYrdr.wgp-5BL.2 (2.42-15.13%) were significant in all tests; and QYrdr.wgp-1BL.1 (1.94-10.19%), QYrdr.wgp-1DS (2.04-27.24%), QYrdr.wgp-3AL (1.78-13.85%) and QYrdr.wgp-6BL.2 (1.69-33.71%) were significant in some of the tests.The three all-stage resistance QTL, QYrdr.wgp-5BL.1 (5.47-36.04%), QYrdr.wgp-5DL (9.27-11.94%) and QYrdr.wgp-6BL.1 (13.07-20.36%), were detected based on reactions in the seedlings tested with certain Pst races.Among the eleven QTL detected in Druchamp, at least three (QYrdr.wgp-5DL for race-specific all-stage resistance and QYrdr.wgp-3AL and QYrdr.wgp-6BL.2 for race non-specific HTAP resistance) are new.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Agricultural Integrated Pest Management, Institute of Plant Protection, Qinghai Academy of Agriculture and Forestry Sciences, Xining, Qinghai, China; Department of Plant Pathology, Washington State University, Pullman, Washington, United States of America; State Key Laboratory of Crop Stress Biology in Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, Shaanxi, China.

ABSTRACT
Winter wheat Druchamp has both high-temperature adult-plant (HTAP) resistance and all-stage resistance to stripe rust caused by Puccinia striiformis f. sp. tritici (Pst). The HTAP resistance in Druchamp is durable as the variety has been resistant in adult-plant stage since it was introduced from France to the United States in late 1940s. To map the quantitative trait loci (QTL) for stripe rust resistance, an F8 recombinant inbred line (RIL) population from cross Druchamp × Michigan Amber was phenotyped for stripe rust response in multiple years in fields under natural infection and with selected Pst races under controlled greenhouse conditions, and genotyped with simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) markers. Composite interval mapping (CIM) identified eight HTAP resistance QTL and three all-stage resistance QTL. Among the eight HTAP resistance QTL, QYrdr.wgp-1BL.2 (explaining 2.36-31.04% variation), QYrdr.wgp-2BL (2.81-15.65%), QYrdr.wgp-5AL (2.27-17.22%) and QYrdr.wgp-5BL.2 (2.42-15.13%) were significant in all tests; and QYrdr.wgp-1BL.1 (1.94-10.19%), QYrdr.wgp-1DS (2.04-27.24%), QYrdr.wgp-3AL (1.78-13.85%) and QYrdr.wgp-6BL.2 (1.69-33.71%) were significant in some of the tests. The three all-stage resistance QTL, QYrdr.wgp-5BL.1 (5.47-36.04%), QYrdr.wgp-5DL (9.27-11.94%) and QYrdr.wgp-6BL.1 (13.07-20.36%), were detected based on reactions in the seedlings tested with certain Pst races. Among the eleven QTL detected in Druchamp, at least three (QYrdr.wgp-5DL for race-specific all-stage resistance and QYrdr.wgp-3AL and QYrdr.wgp-6BL.2 for race non-specific HTAP resistance) are new. All these QTL, especially those for durable HTAP resistance, and their closely linked molecular markers could be useful for developing wheat cultivars with durable resistance to stripe rust.

No MeSH data available.


Related in: MedlinePlus