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Mapping a Large Number of QTL for Durable Resistance to Stripe Rust in Winter Wheat Druchamp Using SSR and SNP Markers.

Hou L, Chen X, Wang M, See DR, Chao S, Bulli P, Jing J - PLoS ONE (2015)

Bottom Line: Among the eight HTAP resistance QTL, QYrdr.wgp-1BL.2 (explaining 2.36-31.04% variation), QYrdr.wgp-2BL (2.81-15.65%), QYrdr.wgp-5AL (2.27-17.22%) and QYrdr.wgp-5BL.2 (2.42-15.13%) were significant in all tests; and QYrdr.wgp-1BL.1 (1.94-10.19%), QYrdr.wgp-1DS (2.04-27.24%), QYrdr.wgp-3AL (1.78-13.85%) and QYrdr.wgp-6BL.2 (1.69-33.71%) were significant in some of the tests.The three all-stage resistance QTL, QYrdr.wgp-5BL.1 (5.47-36.04%), QYrdr.wgp-5DL (9.27-11.94%) and QYrdr.wgp-6BL.1 (13.07-20.36%), were detected based on reactions in the seedlings tested with certain Pst races.Among the eleven QTL detected in Druchamp, at least three (QYrdr.wgp-5DL for race-specific all-stage resistance and QYrdr.wgp-3AL and QYrdr.wgp-6BL.2 for race non-specific HTAP resistance) are new.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Agricultural Integrated Pest Management, Institute of Plant Protection, Qinghai Academy of Agriculture and Forestry Sciences, Xining, Qinghai, China; Department of Plant Pathology, Washington State University, Pullman, Washington, United States of America; State Key Laboratory of Crop Stress Biology in Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, Shaanxi, China.

ABSTRACT
Winter wheat Druchamp has both high-temperature adult-plant (HTAP) resistance and all-stage resistance to stripe rust caused by Puccinia striiformis f. sp. tritici (Pst). The HTAP resistance in Druchamp is durable as the variety has been resistant in adult-plant stage since it was introduced from France to the United States in late 1940s. To map the quantitative trait loci (QTL) for stripe rust resistance, an F8 recombinant inbred line (RIL) population from cross Druchamp × Michigan Amber was phenotyped for stripe rust response in multiple years in fields under natural infection and with selected Pst races under controlled greenhouse conditions, and genotyped with simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) markers. Composite interval mapping (CIM) identified eight HTAP resistance QTL and three all-stage resistance QTL. Among the eight HTAP resistance QTL, QYrdr.wgp-1BL.2 (explaining 2.36-31.04% variation), QYrdr.wgp-2BL (2.81-15.65%), QYrdr.wgp-5AL (2.27-17.22%) and QYrdr.wgp-5BL.2 (2.42-15.13%) were significant in all tests; and QYrdr.wgp-1BL.1 (1.94-10.19%), QYrdr.wgp-1DS (2.04-27.24%), QYrdr.wgp-3AL (1.78-13.85%) and QYrdr.wgp-6BL.2 (1.69-33.71%) were significant in some of the tests. The three all-stage resistance QTL, QYrdr.wgp-5BL.1 (5.47-36.04%), QYrdr.wgp-5DL (9.27-11.94%) and QYrdr.wgp-6BL.1 (13.07-20.36%), were detected based on reactions in the seedlings tested with certain Pst races. Among the eleven QTL detected in Druchamp, at least three (QYrdr.wgp-5DL for race-specific all-stage resistance and QYrdr.wgp-3AL and QYrdr.wgp-6BL.2 for race non-specific HTAP resistance) are new. All these QTL, especially those for durable HTAP resistance, and their closely linked molecular markers could be useful for developing wheat cultivars with durable resistance to stripe rust.

No MeSH data available.


Related in: MedlinePlus

Frequency distributions of mean infection type (IT) values in the Druchamp × Michigan Amber derived recombinant inbred line (RIL) population tested with Puccinia striiformis f. sp. tritici in various environments.IT distribution of: (A) Pullman, WA in 2006, 2010 and 2011; (B) Mt. Vernon, WA in 2005, 2010 and 2011; and (C) greenhouse with races PST-25 and PST-127.
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pone.0126794.g001: Frequency distributions of mean infection type (IT) values in the Druchamp × Michigan Amber derived recombinant inbred line (RIL) population tested with Puccinia striiformis f. sp. tritici in various environments.IT distribution of: (A) Pullman, WA in 2006, 2010 and 2011; (B) Mt. Vernon, WA in 2005, 2010 and 2011; and (C) greenhouse with races PST-25 and PST-127.

Mentions: HTAP resistance was evaluated in fields at Pullman in 2006, 2010 and 2011 and at Mt. Vernon in 2005, 2010 and 2011 under natural infection; and under the greenhouse conditions with races PST-25 and PST-127 which are virulent on the seedlings of Druchamp. In all of the experiments, Druchamp was consistently resistant (IT 1–2), whereas Michigan Amber was susceptible (IT 7–9) (Fig 1A, 1B and 1C). In all experiments, stripe rust developed to adequate levels for high quality phenotypic data as Michigan Amber had more than 90% DS at the second or third time of data recording. The mean rAUDPC values of Druchamp ranged from 2.9 to 28.6%, while Michigan Amber had 90–100% mean rAUDPC values in the experiments (Fig 2A, 2B and 2C). Both IT and rAUDPC data of the RIL population showed continuous distributions, indicating that HTAP resistance in Druchamp was quantitatively inherited (Figs 1 and 2).


Mapping a Large Number of QTL for Durable Resistance to Stripe Rust in Winter Wheat Druchamp Using SSR and SNP Markers.

Hou L, Chen X, Wang M, See DR, Chao S, Bulli P, Jing J - PLoS ONE (2015)

Frequency distributions of mean infection type (IT) values in the Druchamp × Michigan Amber derived recombinant inbred line (RIL) population tested with Puccinia striiformis f. sp. tritici in various environments.IT distribution of: (A) Pullman, WA in 2006, 2010 and 2011; (B) Mt. Vernon, WA in 2005, 2010 and 2011; and (C) greenhouse with races PST-25 and PST-127.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4430513&req=5

pone.0126794.g001: Frequency distributions of mean infection type (IT) values in the Druchamp × Michigan Amber derived recombinant inbred line (RIL) population tested with Puccinia striiformis f. sp. tritici in various environments.IT distribution of: (A) Pullman, WA in 2006, 2010 and 2011; (B) Mt. Vernon, WA in 2005, 2010 and 2011; and (C) greenhouse with races PST-25 and PST-127.
Mentions: HTAP resistance was evaluated in fields at Pullman in 2006, 2010 and 2011 and at Mt. Vernon in 2005, 2010 and 2011 under natural infection; and under the greenhouse conditions with races PST-25 and PST-127 which are virulent on the seedlings of Druchamp. In all of the experiments, Druchamp was consistently resistant (IT 1–2), whereas Michigan Amber was susceptible (IT 7–9) (Fig 1A, 1B and 1C). In all experiments, stripe rust developed to adequate levels for high quality phenotypic data as Michigan Amber had more than 90% DS at the second or third time of data recording. The mean rAUDPC values of Druchamp ranged from 2.9 to 28.6%, while Michigan Amber had 90–100% mean rAUDPC values in the experiments (Fig 2A, 2B and 2C). Both IT and rAUDPC data of the RIL population showed continuous distributions, indicating that HTAP resistance in Druchamp was quantitatively inherited (Figs 1 and 2).

Bottom Line: Among the eight HTAP resistance QTL, QYrdr.wgp-1BL.2 (explaining 2.36-31.04% variation), QYrdr.wgp-2BL (2.81-15.65%), QYrdr.wgp-5AL (2.27-17.22%) and QYrdr.wgp-5BL.2 (2.42-15.13%) were significant in all tests; and QYrdr.wgp-1BL.1 (1.94-10.19%), QYrdr.wgp-1DS (2.04-27.24%), QYrdr.wgp-3AL (1.78-13.85%) and QYrdr.wgp-6BL.2 (1.69-33.71%) were significant in some of the tests.The three all-stage resistance QTL, QYrdr.wgp-5BL.1 (5.47-36.04%), QYrdr.wgp-5DL (9.27-11.94%) and QYrdr.wgp-6BL.1 (13.07-20.36%), were detected based on reactions in the seedlings tested with certain Pst races.Among the eleven QTL detected in Druchamp, at least three (QYrdr.wgp-5DL for race-specific all-stage resistance and QYrdr.wgp-3AL and QYrdr.wgp-6BL.2 for race non-specific HTAP resistance) are new.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Agricultural Integrated Pest Management, Institute of Plant Protection, Qinghai Academy of Agriculture and Forestry Sciences, Xining, Qinghai, China; Department of Plant Pathology, Washington State University, Pullman, Washington, United States of America; State Key Laboratory of Crop Stress Biology in Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, Shaanxi, China.

ABSTRACT
Winter wheat Druchamp has both high-temperature adult-plant (HTAP) resistance and all-stage resistance to stripe rust caused by Puccinia striiformis f. sp. tritici (Pst). The HTAP resistance in Druchamp is durable as the variety has been resistant in adult-plant stage since it was introduced from France to the United States in late 1940s. To map the quantitative trait loci (QTL) for stripe rust resistance, an F8 recombinant inbred line (RIL) population from cross Druchamp × Michigan Amber was phenotyped for stripe rust response in multiple years in fields under natural infection and with selected Pst races under controlled greenhouse conditions, and genotyped with simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) markers. Composite interval mapping (CIM) identified eight HTAP resistance QTL and three all-stage resistance QTL. Among the eight HTAP resistance QTL, QYrdr.wgp-1BL.2 (explaining 2.36-31.04% variation), QYrdr.wgp-2BL (2.81-15.65%), QYrdr.wgp-5AL (2.27-17.22%) and QYrdr.wgp-5BL.2 (2.42-15.13%) were significant in all tests; and QYrdr.wgp-1BL.1 (1.94-10.19%), QYrdr.wgp-1DS (2.04-27.24%), QYrdr.wgp-3AL (1.78-13.85%) and QYrdr.wgp-6BL.2 (1.69-33.71%) were significant in some of the tests. The three all-stage resistance QTL, QYrdr.wgp-5BL.1 (5.47-36.04%), QYrdr.wgp-5DL (9.27-11.94%) and QYrdr.wgp-6BL.1 (13.07-20.36%), were detected based on reactions in the seedlings tested with certain Pst races. Among the eleven QTL detected in Druchamp, at least three (QYrdr.wgp-5DL for race-specific all-stage resistance and QYrdr.wgp-3AL and QYrdr.wgp-6BL.2 for race non-specific HTAP resistance) are new. All these QTL, especially those for durable HTAP resistance, and their closely linked molecular markers could be useful for developing wheat cultivars with durable resistance to stripe rust.

No MeSH data available.


Related in: MedlinePlus