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Characterization and fine mapping of thermo-sensitive chlorophyll deficit mutant1 in rice (Oryza sativa L.).

Liu J, Wang J, Yao X, Zhang Y, Li J, Wang X, Xu Z, Chen W - Breed. Sci. (2015)

Bottom Line: Here, we isolated a spontaneous rice mutant named thermo-sensitive chlorophyll deficit 1 (tscd1) derived from a backcross recombinant inbred line population. tscd1 plants grown normally from the seedling to tiller stages showed yellow leaves with reduced chlorophyll content, but showed no significant differences after the booting stage.Further characterization of the tscd1 gene is underway.Because it has a chlorophyll deficit phenotype before the tiller stage and little influence on growth vigor, it may play a role in ensuring the purity of hybrids.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Crop Physiology, Ecology, Genetics and Breeding, Ministry of Agriculture, Shenyang Agricultural University , 110866 Shenyang , China.

ABSTRACT
Chlorophyll content is one of the most important traits controlling crop biomass and economic yield in rice. Here, we isolated a spontaneous rice mutant named thermo-sensitive chlorophyll deficit 1 (tscd1) derived from a backcross recombinant inbred line population. tscd1 plants grown normally from the seedling to tiller stages showed yellow leaves with reduced chlorophyll content, but showed no significant differences after the booting stage. At temperatures below 22°C, the tscd1 mutant showed the most obvious yellowish phenotype. With increasing temperature, the yellowish leaves gradually turned green and approached a normal wild type color. Wild type and tscd1 mutant plants had obviously different chloroplast structures and photosynthetic pigment precursor contents, which resulted in underdevelopment of chloroplasts and a yellowish phenotype in tscd1. Genetic analysis indicated that the mutant character was controlled by a recessive nuclear gene. Through map-based cloning, we located the tscd1 gene in a 34.95 kb region on the long arm of chromosome 2, containing two BAC clones and eight predicted candidate genes. Further characterization of the tscd1 gene is underway. Because it has a chlorophyll deficit phenotype before the tiller stage and little influence on growth vigor, it may play a role in ensuring the purity of hybrids.

No MeSH data available.


Related in: MedlinePlus

Molecular mapping of the tscd1 gene on rice chromosome 2. A, Primary molecular mapping, B and C, Fine molecular mapping, D, BAC clones and candidate genes were predicated, respectively.
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f8-65_161: Molecular mapping of the tscd1 gene on rice chromosome 2. A, Primary molecular mapping, B and C, Fine molecular mapping, D, BAC clones and candidate genes were predicated, respectively.

Mentions: Genetic analysis showed that the tscd1 phenotype was controlled by a single recessive locus based on the results that all F1 individuals from these crosses had a normal phenotype and that the approximate segregation ratio was 3 (normal) : 1 (yellow) in the F2 populations generated from the crosses tscd1 × Sasanisiki, Sasanisiki × tscd1 and tscd1 × Habataki. Initial mapping of the tscd1 target gene with approximately 500 SSR and Indel markers evenly distributed on the 12 chromosomes was performed using the BSA method to analyze the polymorphic loci among tscd1, Habataki and two DNA pools. Three markers (RM3421, R2M50 and RM425) on the long arm of chromosome 2 were found to be linked with the tscd1 phenotype (Fig. 7, 8A), and 3, 1 and 6 recombinants were detected in 94 F2 mutant phenotype plants in a cross between tscd1 and Habataki.


Characterization and fine mapping of thermo-sensitive chlorophyll deficit mutant1 in rice (Oryza sativa L.).

Liu J, Wang J, Yao X, Zhang Y, Li J, Wang X, Xu Z, Chen W - Breed. Sci. (2015)

Molecular mapping of the tscd1 gene on rice chromosome 2. A, Primary molecular mapping, B and C, Fine molecular mapping, D, BAC clones and candidate genes were predicated, respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4430508&req=5

f8-65_161: Molecular mapping of the tscd1 gene on rice chromosome 2. A, Primary molecular mapping, B and C, Fine molecular mapping, D, BAC clones and candidate genes were predicated, respectively.
Mentions: Genetic analysis showed that the tscd1 phenotype was controlled by a single recessive locus based on the results that all F1 individuals from these crosses had a normal phenotype and that the approximate segregation ratio was 3 (normal) : 1 (yellow) in the F2 populations generated from the crosses tscd1 × Sasanisiki, Sasanisiki × tscd1 and tscd1 × Habataki. Initial mapping of the tscd1 target gene with approximately 500 SSR and Indel markers evenly distributed on the 12 chromosomes was performed using the BSA method to analyze the polymorphic loci among tscd1, Habataki and two DNA pools. Three markers (RM3421, R2M50 and RM425) on the long arm of chromosome 2 were found to be linked with the tscd1 phenotype (Fig. 7, 8A), and 3, 1 and 6 recombinants were detected in 94 F2 mutant phenotype plants in a cross between tscd1 and Habataki.

Bottom Line: Here, we isolated a spontaneous rice mutant named thermo-sensitive chlorophyll deficit 1 (tscd1) derived from a backcross recombinant inbred line population. tscd1 plants grown normally from the seedling to tiller stages showed yellow leaves with reduced chlorophyll content, but showed no significant differences after the booting stage.Further characterization of the tscd1 gene is underway.Because it has a chlorophyll deficit phenotype before the tiller stage and little influence on growth vigor, it may play a role in ensuring the purity of hybrids.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Crop Physiology, Ecology, Genetics and Breeding, Ministry of Agriculture, Shenyang Agricultural University , 110866 Shenyang , China.

ABSTRACT
Chlorophyll content is one of the most important traits controlling crop biomass and economic yield in rice. Here, we isolated a spontaneous rice mutant named thermo-sensitive chlorophyll deficit 1 (tscd1) derived from a backcross recombinant inbred line population. tscd1 plants grown normally from the seedling to tiller stages showed yellow leaves with reduced chlorophyll content, but showed no significant differences after the booting stage. At temperatures below 22°C, the tscd1 mutant showed the most obvious yellowish phenotype. With increasing temperature, the yellowish leaves gradually turned green and approached a normal wild type color. Wild type and tscd1 mutant plants had obviously different chloroplast structures and photosynthetic pigment precursor contents, which resulted in underdevelopment of chloroplasts and a yellowish phenotype in tscd1. Genetic analysis indicated that the mutant character was controlled by a recessive nuclear gene. Through map-based cloning, we located the tscd1 gene in a 34.95 kb region on the long arm of chromosome 2, containing two BAC clones and eight predicted candidate genes. Further characterization of the tscd1 gene is underway. Because it has a chlorophyll deficit phenotype before the tiller stage and little influence on growth vigor, it may play a role in ensuring the purity of hybrids.

No MeSH data available.


Related in: MedlinePlus