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Human Urine Derived Stem Cells in Combination with β-TCP Can Be Applied for Bone Regeneration.

Guan J, Zhang J, Li H, Zhu Z, Guo S, Niu X, Wang Y, Zhang C - PLoS ONE (2015)

Bottom Line: In addition, in our previous studies, USCs have been proved to be able to differentiate into osteoblasts, chondrocytes, and adipocytes.The osteogenic differentiation of USCs within the scaffolds was demonstrated by increased alkaline phosphatase activity and calcium content.Bone regeneration was determined using X-ray, micro-CT, and histologic analyses.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedic Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, People's Republic of China.

ABSTRACT
Bone tissue engineering requires highly proliferative stem cells that are easy to isolate. Human urine stem cells (USCs) are abundant and can be easily harvested without using an invasive procedure. In addition, in our previous studies, USCs have been proved to be able to differentiate into osteoblasts, chondrocytes, and adipocytes. Therefore, USCs may have great potential and advantages to be applied as a cell source for tissue engineering. However, there are no published studies that describe the interactions between USCs and biomaterials and applications of USCs for bone tissue engineering. Therefore, the objective of the present study was to evaluate the interactions between USCs with a typical bone tissue engineering scaffold, beta-Tricalcium Phosphate (β-TCP), and to determine whether the USCs seeded onto β-TCP scaffold can promote bone regeneration in a segmental femoral defect of rats. Primary USCs were isolated from urine and seeded on β-TCP scaffolds. Results showed that USCs remained viable and proliferated within β-TCP. The osteogenic differentiation of USCs within the scaffolds was demonstrated by increased alkaline phosphatase activity and calcium content. Furthermore, β-TCP with adherent USCs (USCs/β-TCP) were implanted in a 6-mm critical size femoral defect of rats for 12 weeks. Bone regeneration was determined using X-ray, micro-CT, and histologic analyses. Results further demonstrated that USCs in the scaffolds could enhance new bone formation, which spanned bone defects in 5 out of 11 rats while β-TCP scaffold alone induced modest bone formation. The current study indicated that the USCs can be used as a cell source for bone tissue engineering as they are compatible with bone tissue engineering scaffolds and can stimulate the regeneration of bone in a critical size bone defect.

No MeSH data available.


Related in: MedlinePlus

Proliferation of USCs on a β-TCP was determined using the CCK-8 assay on days 1, 2, 3, 4, 5, 6, and 7 (a).(b) Comparison of ALP activity after culturing USCs in osteogenesis media. (c) Calcium content quantification of USCs cultured on β-TCP for 7 or 14 days (*P<0.05).
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pone.0125253.g004: Proliferation of USCs on a β-TCP was determined using the CCK-8 assay on days 1, 2, 3, 4, 5, 6, and 7 (a).(b) Comparison of ALP activity after culturing USCs in osteogenesis media. (c) Calcium content quantification of USCs cultured on β-TCP for 7 or 14 days (*P<0.05).

Mentions: SEM was used to evaluate the adhesion of USCs on the β-TCP scaffold. USCs extended pseudopodia and adhered to the inner pore wall 12 h after seeding β-TCP (Fig 2A). The morphology of the USCs resembled that of fibroblasts after 24 h (Fig 2B). On day 3, the USCs reached 70–80% confluence (Fig 2C), and on day 5, most of the pores were covered with clusters of cells (Fig 2D). The viability of the USCs was assessed using the Live/Dead Double staining assay 24 h after the cells were seeded on the scaffolds (Fig 3A). The majority of cells retained their viability on β-TCP after 7 days seeding (Fig 3B). The proliferation of USCs on β-TCP was monitored for 7 days using a CCK-8 kit (Fig 4A). The OD value, which reflects metabolic activity and is directly proportional to the number of living cells, increased from 0.8 to 1.7 and then decreased to 1.3.


Human Urine Derived Stem Cells in Combination with β-TCP Can Be Applied for Bone Regeneration.

Guan J, Zhang J, Li H, Zhu Z, Guo S, Niu X, Wang Y, Zhang C - PLoS ONE (2015)

Proliferation of USCs on a β-TCP was determined using the CCK-8 assay on days 1, 2, 3, 4, 5, 6, and 7 (a).(b) Comparison of ALP activity after culturing USCs in osteogenesis media. (c) Calcium content quantification of USCs cultured on β-TCP for 7 or 14 days (*P<0.05).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4430281&req=5

pone.0125253.g004: Proliferation of USCs on a β-TCP was determined using the CCK-8 assay on days 1, 2, 3, 4, 5, 6, and 7 (a).(b) Comparison of ALP activity after culturing USCs in osteogenesis media. (c) Calcium content quantification of USCs cultured on β-TCP for 7 or 14 days (*P<0.05).
Mentions: SEM was used to evaluate the adhesion of USCs on the β-TCP scaffold. USCs extended pseudopodia and adhered to the inner pore wall 12 h after seeding β-TCP (Fig 2A). The morphology of the USCs resembled that of fibroblasts after 24 h (Fig 2B). On day 3, the USCs reached 70–80% confluence (Fig 2C), and on day 5, most of the pores were covered with clusters of cells (Fig 2D). The viability of the USCs was assessed using the Live/Dead Double staining assay 24 h after the cells were seeded on the scaffolds (Fig 3A). The majority of cells retained their viability on β-TCP after 7 days seeding (Fig 3B). The proliferation of USCs on β-TCP was monitored for 7 days using a CCK-8 kit (Fig 4A). The OD value, which reflects metabolic activity and is directly proportional to the number of living cells, increased from 0.8 to 1.7 and then decreased to 1.3.

Bottom Line: In addition, in our previous studies, USCs have been proved to be able to differentiate into osteoblasts, chondrocytes, and adipocytes.The osteogenic differentiation of USCs within the scaffolds was demonstrated by increased alkaline phosphatase activity and calcium content.Bone regeneration was determined using X-ray, micro-CT, and histologic analyses.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedic Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, People's Republic of China.

ABSTRACT
Bone tissue engineering requires highly proliferative stem cells that are easy to isolate. Human urine stem cells (USCs) are abundant and can be easily harvested without using an invasive procedure. In addition, in our previous studies, USCs have been proved to be able to differentiate into osteoblasts, chondrocytes, and adipocytes. Therefore, USCs may have great potential and advantages to be applied as a cell source for tissue engineering. However, there are no published studies that describe the interactions between USCs and biomaterials and applications of USCs for bone tissue engineering. Therefore, the objective of the present study was to evaluate the interactions between USCs with a typical bone tissue engineering scaffold, beta-Tricalcium Phosphate (β-TCP), and to determine whether the USCs seeded onto β-TCP scaffold can promote bone regeneration in a segmental femoral defect of rats. Primary USCs were isolated from urine and seeded on β-TCP scaffolds. Results showed that USCs remained viable and proliferated within β-TCP. The osteogenic differentiation of USCs within the scaffolds was demonstrated by increased alkaline phosphatase activity and calcium content. Furthermore, β-TCP with adherent USCs (USCs/β-TCP) were implanted in a 6-mm critical size femoral defect of rats for 12 weeks. Bone regeneration was determined using X-ray, micro-CT, and histologic analyses. Results further demonstrated that USCs in the scaffolds could enhance new bone formation, which spanned bone defects in 5 out of 11 rats while β-TCP scaffold alone induced modest bone formation. The current study indicated that the USCs can be used as a cell source for bone tissue engineering as they are compatible with bone tissue engineering scaffolds and can stimulate the regeneration of bone in a critical size bone defect.

No MeSH data available.


Related in: MedlinePlus