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Human Urine Derived Stem Cells in Combination with β-TCP Can Be Applied for Bone Regeneration.

Guan J, Zhang J, Li H, Zhu Z, Guo S, Niu X, Wang Y, Zhang C - PLoS ONE (2015)

Bottom Line: In addition, in our previous studies, USCs have been proved to be able to differentiate into osteoblasts, chondrocytes, and adipocytes.The osteogenic differentiation of USCs within the scaffolds was demonstrated by increased alkaline phosphatase activity and calcium content.Bone regeneration was determined using X-ray, micro-CT, and histologic analyses.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedic Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, People's Republic of China.

ABSTRACT
Bone tissue engineering requires highly proliferative stem cells that are easy to isolate. Human urine stem cells (USCs) are abundant and can be easily harvested without using an invasive procedure. In addition, in our previous studies, USCs have been proved to be able to differentiate into osteoblasts, chondrocytes, and adipocytes. Therefore, USCs may have great potential and advantages to be applied as a cell source for tissue engineering. However, there are no published studies that describe the interactions between USCs and biomaterials and applications of USCs for bone tissue engineering. Therefore, the objective of the present study was to evaluate the interactions between USCs with a typical bone tissue engineering scaffold, beta-Tricalcium Phosphate (β-TCP), and to determine whether the USCs seeded onto β-TCP scaffold can promote bone regeneration in a segmental femoral defect of rats. Primary USCs were isolated from urine and seeded on β-TCP scaffolds. Results showed that USCs remained viable and proliferated within β-TCP. The osteogenic differentiation of USCs within the scaffolds was demonstrated by increased alkaline phosphatase activity and calcium content. Furthermore, β-TCP with adherent USCs (USCs/β-TCP) were implanted in a 6-mm critical size femoral defect of rats for 12 weeks. Bone regeneration was determined using X-ray, micro-CT, and histologic analyses. Results further demonstrated that USCs in the scaffolds could enhance new bone formation, which spanned bone defects in 5 out of 11 rats while β-TCP scaffold alone induced modest bone formation. The current study indicated that the USCs can be used as a cell source for bone tissue engineering as they are compatible with bone tissue engineering scaffolds and can stimulate the regeneration of bone in a critical size bone defect.

No MeSH data available.


Related in: MedlinePlus

Biological characteristics of USCs.(a) Primary USCs exhibited a spindle-shaped morphology. Osteogenic differentiation was confirmed using Alizarin Red (b) and von Kossa (c) staining, (d) Toluidine blue staining revealed chondrogenic differentiation, and (e) adipogenic differentiation was verified using Oil red O staining. (f) Flow cytometric analysis demonstrates that USCs expressed markers associated with mesenchymal stem cells and did not express HLA-DR. Black traces indicate isotype controls and gray traces show the level of cell-surface expression. Scale bar = 400 μm.
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pone.0125253.g001: Biological characteristics of USCs.(a) Primary USCs exhibited a spindle-shaped morphology. Osteogenic differentiation was confirmed using Alizarin Red (b) and von Kossa (c) staining, (d) Toluidine blue staining revealed chondrogenic differentiation, and (e) adipogenic differentiation was verified using Oil red O staining. (f) Flow cytometric analysis demonstrates that USCs expressed markers associated with mesenchymal stem cells and did not express HLA-DR. Black traces indicate isotype controls and gray traces show the level of cell-surface expression. Scale bar = 400 μm.

Mentions: Typical USCs colony-forming units were detected 7–10 days after culture. Observation obtained using an inverted phase-contrast light microscope showed that the morphology of freshly isolated colonies was fibroblast-like (Fig 1A). The USCs were positive for CD29, CD44, CD73 and CD90 and negative for CD34, CD45, CD133, and HLA-DR. (Fig 1F). These finding indicate that the phenotype of USCs was similar to that of MSCs.


Human Urine Derived Stem Cells in Combination with β-TCP Can Be Applied for Bone Regeneration.

Guan J, Zhang J, Li H, Zhu Z, Guo S, Niu X, Wang Y, Zhang C - PLoS ONE (2015)

Biological characteristics of USCs.(a) Primary USCs exhibited a spindle-shaped morphology. Osteogenic differentiation was confirmed using Alizarin Red (b) and von Kossa (c) staining, (d) Toluidine blue staining revealed chondrogenic differentiation, and (e) adipogenic differentiation was verified using Oil red O staining. (f) Flow cytometric analysis demonstrates that USCs expressed markers associated with mesenchymal stem cells and did not express HLA-DR. Black traces indicate isotype controls and gray traces show the level of cell-surface expression. Scale bar = 400 μm.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4430281&req=5

pone.0125253.g001: Biological characteristics of USCs.(a) Primary USCs exhibited a spindle-shaped morphology. Osteogenic differentiation was confirmed using Alizarin Red (b) and von Kossa (c) staining, (d) Toluidine blue staining revealed chondrogenic differentiation, and (e) adipogenic differentiation was verified using Oil red O staining. (f) Flow cytometric analysis demonstrates that USCs expressed markers associated with mesenchymal stem cells and did not express HLA-DR. Black traces indicate isotype controls and gray traces show the level of cell-surface expression. Scale bar = 400 μm.
Mentions: Typical USCs colony-forming units were detected 7–10 days after culture. Observation obtained using an inverted phase-contrast light microscope showed that the morphology of freshly isolated colonies was fibroblast-like (Fig 1A). The USCs were positive for CD29, CD44, CD73 and CD90 and negative for CD34, CD45, CD133, and HLA-DR. (Fig 1F). These finding indicate that the phenotype of USCs was similar to that of MSCs.

Bottom Line: In addition, in our previous studies, USCs have been proved to be able to differentiate into osteoblasts, chondrocytes, and adipocytes.The osteogenic differentiation of USCs within the scaffolds was demonstrated by increased alkaline phosphatase activity and calcium content.Bone regeneration was determined using X-ray, micro-CT, and histologic analyses.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedic Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, People's Republic of China.

ABSTRACT
Bone tissue engineering requires highly proliferative stem cells that are easy to isolate. Human urine stem cells (USCs) are abundant and can be easily harvested without using an invasive procedure. In addition, in our previous studies, USCs have been proved to be able to differentiate into osteoblasts, chondrocytes, and adipocytes. Therefore, USCs may have great potential and advantages to be applied as a cell source for tissue engineering. However, there are no published studies that describe the interactions between USCs and biomaterials and applications of USCs for bone tissue engineering. Therefore, the objective of the present study was to evaluate the interactions between USCs with a typical bone tissue engineering scaffold, beta-Tricalcium Phosphate (β-TCP), and to determine whether the USCs seeded onto β-TCP scaffold can promote bone regeneration in a segmental femoral defect of rats. Primary USCs were isolated from urine and seeded on β-TCP scaffolds. Results showed that USCs remained viable and proliferated within β-TCP. The osteogenic differentiation of USCs within the scaffolds was demonstrated by increased alkaline phosphatase activity and calcium content. Furthermore, β-TCP with adherent USCs (USCs/β-TCP) were implanted in a 6-mm critical size femoral defect of rats for 12 weeks. Bone regeneration was determined using X-ray, micro-CT, and histologic analyses. Results further demonstrated that USCs in the scaffolds could enhance new bone formation, which spanned bone defects in 5 out of 11 rats while β-TCP scaffold alone induced modest bone formation. The current study indicated that the USCs can be used as a cell source for bone tissue engineering as they are compatible with bone tissue engineering scaffolds and can stimulate the regeneration of bone in a critical size bone defect.

No MeSH data available.


Related in: MedlinePlus