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S-adenosylmethionine blocks osteosarcoma cells proliferation and invasion in vitro and tumor metastasis in vivo: therapeutic and diagnostic clinical applications.

Parashar S, Cheishvili D, Arakelian A, Hussain Z, Tanvir I, Khan HA, Szyf M, Rabbani SA - Cancer Med (2015)

Bottom Line: Treatment with SAM resulted in a dose-dependent inhibition of tumor cell proliferation, invasion, cell migration, and cell cycle characteristics.Immunohistochemical analysis of normal human bone and tissue array from OS patients showed significantly high levels of expression of one of the identified gene platelet-derived growth factor alpha (PDGFA).These studies provide a possible mechanism for the role of DNA demethylation in the development and metastasis of OS to provide a rationale for the use of hypermethylation therapy for OS patients and identify new targets for monitoring OS development and progression.

View Article: PubMed Central - PubMed

Affiliation: Department of Medicine, McGill University Health Centre, Montreal, QC, Canada.

No MeSH data available.


Related in: MedlinePlus

Effect of S-adenosylmethionine (SAM) on osteosarcoma (OS) cells colony formation and cycle kinetics in vitro. LM-7 and MG-63 cells were plated onto soft agar for anchorage independent growth in the presence of 150 μmol/L S-adenosylhomocystine as control (SAH) or SAM (75 and 150 μmol/L). Number of colonies was counted as described in Materials and Methods section (A). LM-7 and MG-63 cells were treated with 150 μmol/L of SAH as control (SAH) or SAM (75 and 150 μmol/L). Treated cells were then fixed and stained with propidium iodide. FACS analysis was performed as described in Materials and Methods section (B). Results are presented as the mean ± SEM of two different experiments in duplicate from control and experimental cells. Significant differences from the control (SAH) is represented by an asterisk (P < 0.05).
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fig03: Effect of S-adenosylmethionine (SAM) on osteosarcoma (OS) cells colony formation and cycle kinetics in vitro. LM-7 and MG-63 cells were plated onto soft agar for anchorage independent growth in the presence of 150 μmol/L S-adenosylhomocystine as control (SAH) or SAM (75 and 150 μmol/L). Number of colonies was counted as described in Materials and Methods section (A). LM-7 and MG-63 cells were treated with 150 μmol/L of SAH as control (SAH) or SAM (75 and 150 μmol/L). Treated cells were then fixed and stained with propidium iodide. FACS analysis was performed as described in Materials and Methods section (B). Results are presented as the mean ± SEM of two different experiments in duplicate from control and experimental cells. Significant differences from the control (SAH) is represented by an asterisk (P < 0.05).

Mentions: Tumor cell's ability to form colonies in soft agar is an index of their aggressive potential. We therefore examined the effect of SAM on the number of colonies formed by LM-7 and MG-63 cells. Following treatment of these cells with (75 and 150 μmol/L) of SAM, a significant and dose-dependent decrease in the number of colonies formed was observed compared to control (SAH-treated) group of cells (Fig.3A).


S-adenosylmethionine blocks osteosarcoma cells proliferation and invasion in vitro and tumor metastasis in vivo: therapeutic and diagnostic clinical applications.

Parashar S, Cheishvili D, Arakelian A, Hussain Z, Tanvir I, Khan HA, Szyf M, Rabbani SA - Cancer Med (2015)

Effect of S-adenosylmethionine (SAM) on osteosarcoma (OS) cells colony formation and cycle kinetics in vitro. LM-7 and MG-63 cells were plated onto soft agar for anchorage independent growth in the presence of 150 μmol/L S-adenosylhomocystine as control (SAH) or SAM (75 and 150 μmol/L). Number of colonies was counted as described in Materials and Methods section (A). LM-7 and MG-63 cells were treated with 150 μmol/L of SAH as control (SAH) or SAM (75 and 150 μmol/L). Treated cells were then fixed and stained with propidium iodide. FACS analysis was performed as described in Materials and Methods section (B). Results are presented as the mean ± SEM of two different experiments in duplicate from control and experimental cells. Significant differences from the control (SAH) is represented by an asterisk (P < 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4430266&req=5

fig03: Effect of S-adenosylmethionine (SAM) on osteosarcoma (OS) cells colony formation and cycle kinetics in vitro. LM-7 and MG-63 cells were plated onto soft agar for anchorage independent growth in the presence of 150 μmol/L S-adenosylhomocystine as control (SAH) or SAM (75 and 150 μmol/L). Number of colonies was counted as described in Materials and Methods section (A). LM-7 and MG-63 cells were treated with 150 μmol/L of SAH as control (SAH) or SAM (75 and 150 μmol/L). Treated cells were then fixed and stained with propidium iodide. FACS analysis was performed as described in Materials and Methods section (B). Results are presented as the mean ± SEM of two different experiments in duplicate from control and experimental cells. Significant differences from the control (SAH) is represented by an asterisk (P < 0.05).
Mentions: Tumor cell's ability to form colonies in soft agar is an index of their aggressive potential. We therefore examined the effect of SAM on the number of colonies formed by LM-7 and MG-63 cells. Following treatment of these cells with (75 and 150 μmol/L) of SAM, a significant and dose-dependent decrease in the number of colonies formed was observed compared to control (SAH-treated) group of cells (Fig.3A).

Bottom Line: Treatment with SAM resulted in a dose-dependent inhibition of tumor cell proliferation, invasion, cell migration, and cell cycle characteristics.Immunohistochemical analysis of normal human bone and tissue array from OS patients showed significantly high levels of expression of one of the identified gene platelet-derived growth factor alpha (PDGFA).These studies provide a possible mechanism for the role of DNA demethylation in the development and metastasis of OS to provide a rationale for the use of hypermethylation therapy for OS patients and identify new targets for monitoring OS development and progression.

View Article: PubMed Central - PubMed

Affiliation: Department of Medicine, McGill University Health Centre, Montreal, QC, Canada.

No MeSH data available.


Related in: MedlinePlus