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Vitamin D enhances the efficacy of photodynamic therapy in a murine model of breast cancer.

Rollakanti KR, Anand S, Maytin EV - Cancer Med (2015)

Bottom Line: Cutaneous metastasis occurs more frequently in breast cancer than in any other malignancy in women, causing significant morbidity.Bioluminescence imaging in vivo and immunohistochemical staining confirmed that tumor-specific cell death after ALA-PDT was markedly enhanced (36.8 ± 7.4-fold increase in TUNEL-positive nuclei; radiance decreased to 14% of control) in Vit D pretreated tumors as compared to vehicle-pretreated tumors.The observed enhancement of tumor responses to ALA-PDT after low, nontoxic doses of Vit D supports a new combination approach that deserves consideration in the clinical setting, and offers potential for improved remission of cutaneous breast cancer metastases.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemical and Biomedical Engineering, Cleveland State University, 2121 Euclid Avenue, Cleveland, Ohio, 44115.

No MeSH data available.


Related in: MedlinePlus

Vit D preconditioning increases the status of proliferation and differentiation in MDA-MB-231-luc tumor cells. Images show paraffin sections of the tumors, immunostained with antisera to (A), E-Cadherin (red fluorescence), and (B), Ki-67 (green fluorescence). Quantification of relative staining intensity of (C), E-Cadherin and (D), Ki-67 from multiple histologic specimens of the tumors. Scale bars, 50 μm. Mean ± SEM from six tumors per condition. The P values from unpaired two-sided t-tests are indicated.
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fig04: Vit D preconditioning increases the status of proliferation and differentiation in MDA-MB-231-luc tumor cells. Images show paraffin sections of the tumors, immunostained with antisera to (A), E-Cadherin (red fluorescence), and (B), Ki-67 (green fluorescence). Quantification of relative staining intensity of (C), E-Cadherin and (D), Ki-67 from multiple histologic specimens of the tumors. Scale bars, 50 μm. Mean ± SEM from six tumors per condition. The P values from unpaired two-sided t-tests are indicated.

Mentions: To examine the effect of Vit D pretreatment on differentiation and proliferation status, tumor tissues were stained for E-cadherin and Ki-67, respectively. When compared with saline-treated control tumors, E-cadherin expression was significantly increased in Vit D pretreated tumors (Fig.4A). E-cadherin was mainly expressed in cells within ductal structures in the tumors that received Vit D, whereas E-cadherin in saline-treated tumors was undetectable (Fig.4A). Expression of nuclear Ki-67 was also induced, being observed in the majority of cells within tumors receiving Vit D preconditioning (Fig.4B). Quantitatively, E-cadherin and Ki-67 were increased 9.6 ± 1.7-fold and 10.7 ± 2.8-fold, respectively, in Vit D-preconditioned tumors relative to nonconditioned controls (Fig.4C and D).


Vitamin D enhances the efficacy of photodynamic therapy in a murine model of breast cancer.

Rollakanti KR, Anand S, Maytin EV - Cancer Med (2015)

Vit D preconditioning increases the status of proliferation and differentiation in MDA-MB-231-luc tumor cells. Images show paraffin sections of the tumors, immunostained with antisera to (A), E-Cadherin (red fluorescence), and (B), Ki-67 (green fluorescence). Quantification of relative staining intensity of (C), E-Cadherin and (D), Ki-67 from multiple histologic specimens of the tumors. Scale bars, 50 μm. Mean ± SEM from six tumors per condition. The P values from unpaired two-sided t-tests are indicated.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4430256&req=5

fig04: Vit D preconditioning increases the status of proliferation and differentiation in MDA-MB-231-luc tumor cells. Images show paraffin sections of the tumors, immunostained with antisera to (A), E-Cadherin (red fluorescence), and (B), Ki-67 (green fluorescence). Quantification of relative staining intensity of (C), E-Cadherin and (D), Ki-67 from multiple histologic specimens of the tumors. Scale bars, 50 μm. Mean ± SEM from six tumors per condition. The P values from unpaired two-sided t-tests are indicated.
Mentions: To examine the effect of Vit D pretreatment on differentiation and proliferation status, tumor tissues were stained for E-cadherin and Ki-67, respectively. When compared with saline-treated control tumors, E-cadherin expression was significantly increased in Vit D pretreated tumors (Fig.4A). E-cadherin was mainly expressed in cells within ductal structures in the tumors that received Vit D, whereas E-cadherin in saline-treated tumors was undetectable (Fig.4A). Expression of nuclear Ki-67 was also induced, being observed in the majority of cells within tumors receiving Vit D preconditioning (Fig.4B). Quantitatively, E-cadherin and Ki-67 were increased 9.6 ± 1.7-fold and 10.7 ± 2.8-fold, respectively, in Vit D-preconditioned tumors relative to nonconditioned controls (Fig.4C and D).

Bottom Line: Cutaneous metastasis occurs more frequently in breast cancer than in any other malignancy in women, causing significant morbidity.Bioluminescence imaging in vivo and immunohistochemical staining confirmed that tumor-specific cell death after ALA-PDT was markedly enhanced (36.8 ± 7.4-fold increase in TUNEL-positive nuclei; radiance decreased to 14% of control) in Vit D pretreated tumors as compared to vehicle-pretreated tumors.The observed enhancement of tumor responses to ALA-PDT after low, nontoxic doses of Vit D supports a new combination approach that deserves consideration in the clinical setting, and offers potential for improved remission of cutaneous breast cancer metastases.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemical and Biomedical Engineering, Cleveland State University, 2121 Euclid Avenue, Cleveland, Ohio, 44115.

No MeSH data available.


Related in: MedlinePlus