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The Phosphate Transporter Gene OsPht1;4 Is Involved in Phosphate Homeostasis in Rice.

Ye Y, Yuan J, Chang X, Yang M, Zhang L, Lu K, Lian X - PLoS ONE (2015)

Bottom Line: Overexpressing OsPT4 resulted in significant higher Pi accumulation in roots, straw and brown rice, and suppression of OsPT4 caused decreased Pi concentration in straw and brown rice.The transcript level of OsPT4 increased significantly both in shoots and roots with a long time Pi starvation.We concluded that OsPT4 is a functional Pi-influx transporter involved in Pi absorption in rice that might play a role in Pi translocation.

View Article: PubMed Central - PubMed

Affiliation: National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan, 430070, P.R. China.

ABSTRACT
A total of 13 phosphate transporters in rice (Oryza sative) have been identified as belonging to the Pht1 family, which mediates inorganic phosphate (Pi) uptake and transport. We report the biological property and physiological role of OsPht1;4 (OsPT4). Overexpressing OsPT4 resulted in significant higher Pi accumulation in roots, straw and brown rice, and suppression of OsPT4 caused decreased Pi concentration in straw and brown rice. Expression of the β-glucuronidase reporter gene driven by the OsPT4 promoter showed that OsPT4 is expressed in roots, leaves, ligules, stamens, and caryopses under sufficient Pi conditions, consistent with the expression profile showing that OsPT4 has high expression in roots and flag leaves. The transcript level of OsPT4 increased significantly both in shoots and roots with a long time Pi starvation. OsPT4 encoded a plasma membrane-localized protein and was able to complement the function of the Pi transporter gene PHO84 in yeast. We concluded that OsPT4 is a functional Pi-influx transporter involved in Pi absorption in rice that might play a role in Pi translocation. This study will enrich our understanding about the physiological function of rice Pht1 family genes.

No MeSH data available.


Related in: MedlinePlus

Pi concentration of the wild-type and transgenic plants in field.The rice including wild type and transgenic plants were grown in field. When plants grown to maturity, the phosphate concentration of straws and brown rice were measured. Data are means ± SD of five biological replicates. Values are significantly different from those of wild-type: *P<0.05 and **P<0.01. (one-way ANOVA).
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pone.0126186.g006: Pi concentration of the wild-type and transgenic plants in field.The rice including wild type and transgenic plants were grown in field. When plants grown to maturity, the phosphate concentration of straws and brown rice were measured. Data are means ± SD of five biological replicates. Values are significantly different from those of wild-type: *P<0.05 and **P<0.01. (one-way ANOVA).

Mentions: The fact that the Pi concentration of OsPT4-Oe plants accumulated in roots suggested that OsPT4 may play an important role in rice Pi uptake. To understand the function of OsPT4 in Pi uptake, we measured the Pi concentration of wild type and OsPT4 transgenic plants grown in field with normal P concentration (available Pi of 14mg/kg soil). The Pi concentration of transgenic plants in both straw and brown rice changed (Fig 6). The Pi concentration doubled in straw of the OsPT4-Oe plants compared with the wild type plants, whereas the straw Pi concentration in OsPT4-Ri decreased to about half of that in wild type plants. Furthermore, the Pi concentration of brown rice in OsPT4-Oe plants increased 26% compared with wild type plants, whereas the Pi concentrations in OsPT4-Ri plants were slightly decreased. The biomasses of wild type and OsPT4 transgenic plants had no significant difference (S3 Fig).


The Phosphate Transporter Gene OsPht1;4 Is Involved in Phosphate Homeostasis in Rice.

Ye Y, Yuan J, Chang X, Yang M, Zhang L, Lu K, Lian X - PLoS ONE (2015)

Pi concentration of the wild-type and transgenic plants in field.The rice including wild type and transgenic plants were grown in field. When plants grown to maturity, the phosphate concentration of straws and brown rice were measured. Data are means ± SD of five biological replicates. Values are significantly different from those of wild-type: *P<0.05 and **P<0.01. (one-way ANOVA).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4430236&req=5

pone.0126186.g006: Pi concentration of the wild-type and transgenic plants in field.The rice including wild type and transgenic plants were grown in field. When plants grown to maturity, the phosphate concentration of straws and brown rice were measured. Data are means ± SD of five biological replicates. Values are significantly different from those of wild-type: *P<0.05 and **P<0.01. (one-way ANOVA).
Mentions: The fact that the Pi concentration of OsPT4-Oe plants accumulated in roots suggested that OsPT4 may play an important role in rice Pi uptake. To understand the function of OsPT4 in Pi uptake, we measured the Pi concentration of wild type and OsPT4 transgenic plants grown in field with normal P concentration (available Pi of 14mg/kg soil). The Pi concentration of transgenic plants in both straw and brown rice changed (Fig 6). The Pi concentration doubled in straw of the OsPT4-Oe plants compared with the wild type plants, whereas the straw Pi concentration in OsPT4-Ri decreased to about half of that in wild type plants. Furthermore, the Pi concentration of brown rice in OsPT4-Oe plants increased 26% compared with wild type plants, whereas the Pi concentrations in OsPT4-Ri plants were slightly decreased. The biomasses of wild type and OsPT4 transgenic plants had no significant difference (S3 Fig).

Bottom Line: Overexpressing OsPT4 resulted in significant higher Pi accumulation in roots, straw and brown rice, and suppression of OsPT4 caused decreased Pi concentration in straw and brown rice.The transcript level of OsPT4 increased significantly both in shoots and roots with a long time Pi starvation.We concluded that OsPT4 is a functional Pi-influx transporter involved in Pi absorption in rice that might play a role in Pi translocation.

View Article: PubMed Central - PubMed

Affiliation: National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan, 430070, P.R. China.

ABSTRACT
A total of 13 phosphate transporters in rice (Oryza sative) have been identified as belonging to the Pht1 family, which mediates inorganic phosphate (Pi) uptake and transport. We report the biological property and physiological role of OsPht1;4 (OsPT4). Overexpressing OsPT4 resulted in significant higher Pi accumulation in roots, straw and brown rice, and suppression of OsPT4 caused decreased Pi concentration in straw and brown rice. Expression of the β-glucuronidase reporter gene driven by the OsPT4 promoter showed that OsPT4 is expressed in roots, leaves, ligules, stamens, and caryopses under sufficient Pi conditions, consistent with the expression profile showing that OsPT4 has high expression in roots and flag leaves. The transcript level of OsPT4 increased significantly both in shoots and roots with a long time Pi starvation. OsPT4 encoded a plasma membrane-localized protein and was able to complement the function of the Pi transporter gene PHO84 in yeast. We concluded that OsPT4 is a functional Pi-influx transporter involved in Pi absorption in rice that might play a role in Pi translocation. This study will enrich our understanding about the physiological function of rice Pht1 family genes.

No MeSH data available.


Related in: MedlinePlus