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The Phosphate Transporter Gene OsPht1;4 Is Involved in Phosphate Homeostasis in Rice.

Ye Y, Yuan J, Chang X, Yang M, Zhang L, Lu K, Lian X - PLoS ONE (2015)

Bottom Line: Overexpressing OsPT4 resulted in significant higher Pi accumulation in roots, straw and brown rice, and suppression of OsPT4 caused decreased Pi concentration in straw and brown rice.The transcript level of OsPT4 increased significantly both in shoots and roots with a long time Pi starvation.We concluded that OsPT4 is a functional Pi-influx transporter involved in Pi absorption in rice that might play a role in Pi translocation.

View Article: PubMed Central - PubMed

Affiliation: National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan, 430070, P.R. China.

ABSTRACT
A total of 13 phosphate transporters in rice (Oryza sative) have been identified as belonging to the Pht1 family, which mediates inorganic phosphate (Pi) uptake and transport. We report the biological property and physiological role of OsPht1;4 (OsPT4). Overexpressing OsPT4 resulted in significant higher Pi accumulation in roots, straw and brown rice, and suppression of OsPT4 caused decreased Pi concentration in straw and brown rice. Expression of the β-glucuronidase reporter gene driven by the OsPT4 promoter showed that OsPT4 is expressed in roots, leaves, ligules, stamens, and caryopses under sufficient Pi conditions, consistent with the expression profile showing that OsPT4 has high expression in roots and flag leaves. The transcript level of OsPT4 increased significantly both in shoots and roots with a long time Pi starvation. OsPT4 encoded a plasma membrane-localized protein and was able to complement the function of the Pi transporter gene PHO84 in yeast. We concluded that OsPT4 is a functional Pi-influx transporter involved in Pi absorption in rice that might play a role in Pi translocation. This study will enrich our understanding about the physiological function of rice Pht1 family genes.

No MeSH data available.


Related in: MedlinePlus

Function expression of OsPT4 in yeast.The yeast cells were grown in solution that had been stained for acidification. The pH indicator, bromocresol purple, did not change from blue to yellow until the yeast cells had significant growth in culture. The medium contained 0 μM and 75 μM Pi, respectively. A: Color shifts in wild-type (WT), yeast strain pho84 (control), and pho84+OsPT4, which express OsPT4 in pho84 grown on synthetic defined (SD) Ura mediums. B: Growth conditions of wild-type, pho84, and pho84 transformed with pYES2-OsPT4 generated in a 24-hour liquid culture under 0μM and 75μM Pi conditions. Statistically significant differences are indicated: **p<0.01, Student’s one-way ANOVA analysis.
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pone.0126186.g004: Function expression of OsPT4 in yeast.The yeast cells were grown in solution that had been stained for acidification. The pH indicator, bromocresol purple, did not change from blue to yellow until the yeast cells had significant growth in culture. The medium contained 0 μM and 75 μM Pi, respectively. A: Color shifts in wild-type (WT), yeast strain pho84 (control), and pho84+OsPT4, which express OsPT4 in pho84 grown on synthetic defined (SD) Ura mediums. B: Growth conditions of wild-type, pho84, and pho84 transformed with pYES2-OsPT4 generated in a 24-hour liquid culture under 0μM and 75μM Pi conditions. Statistically significant differences are indicated: **p<0.01, Student’s one-way ANOVA analysis.

Mentions: Because OsPT4 belongs to the Pht1 family, which is always involved in Pi transport, a functional complementation analysis of the yeast mutant was performed. Yeast cell growth was well-correlated with acidification of the liquid growth media, as indicated by the color shift of the pH indicator bromocresol purple. Bromocresol purple changes its color from blue to yellow at pH from 6.3 to 5.8. We expressed the OsPT4 in the yeast mutant strain Δpho84, which is defective in high-affinity Pi transport. The mutant yeast containing OsPT4 and empty vector were grown in a yeast nitrogen base medium with various concentrations of Pi over 10 hours at pH 7.0 The pH indicator did not change from blue to yellow until the yeast cells had significant growth in culture. The colors of these three yeasts containing wild-type and Δpho84 with and without OsPT4 were all blue at the Pi concentration of 0 μM, revealing that little or no yeast cells had grown in medium without Pi. After increasing the Pi concentration of the medium to 75 μM, the color changed to yellow, with wild-type and Δpho84 expressing OsPT4 rather than mutant yeast, suggesting that the expression of OsPT4 restored the yeast growth rate (Fig 4A). To further confirm this result, absorbance of these different yeasts at 600 nm was tested and showed that OsPT4 could partially complement the function of yeast Pi transporter PHO84 (Fig 4B).


The Phosphate Transporter Gene OsPht1;4 Is Involved in Phosphate Homeostasis in Rice.

Ye Y, Yuan J, Chang X, Yang M, Zhang L, Lu K, Lian X - PLoS ONE (2015)

Function expression of OsPT4 in yeast.The yeast cells were grown in solution that had been stained for acidification. The pH indicator, bromocresol purple, did not change from blue to yellow until the yeast cells had significant growth in culture. The medium contained 0 μM and 75 μM Pi, respectively. A: Color shifts in wild-type (WT), yeast strain pho84 (control), and pho84+OsPT4, which express OsPT4 in pho84 grown on synthetic defined (SD) Ura mediums. B: Growth conditions of wild-type, pho84, and pho84 transformed with pYES2-OsPT4 generated in a 24-hour liquid culture under 0μM and 75μM Pi conditions. Statistically significant differences are indicated: **p<0.01, Student’s one-way ANOVA analysis.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4430236&req=5

pone.0126186.g004: Function expression of OsPT4 in yeast.The yeast cells were grown in solution that had been stained for acidification. The pH indicator, bromocresol purple, did not change from blue to yellow until the yeast cells had significant growth in culture. The medium contained 0 μM and 75 μM Pi, respectively. A: Color shifts in wild-type (WT), yeast strain pho84 (control), and pho84+OsPT4, which express OsPT4 in pho84 grown on synthetic defined (SD) Ura mediums. B: Growth conditions of wild-type, pho84, and pho84 transformed with pYES2-OsPT4 generated in a 24-hour liquid culture under 0μM and 75μM Pi conditions. Statistically significant differences are indicated: **p<0.01, Student’s one-way ANOVA analysis.
Mentions: Because OsPT4 belongs to the Pht1 family, which is always involved in Pi transport, a functional complementation analysis of the yeast mutant was performed. Yeast cell growth was well-correlated with acidification of the liquid growth media, as indicated by the color shift of the pH indicator bromocresol purple. Bromocresol purple changes its color from blue to yellow at pH from 6.3 to 5.8. We expressed the OsPT4 in the yeast mutant strain Δpho84, which is defective in high-affinity Pi transport. The mutant yeast containing OsPT4 and empty vector were grown in a yeast nitrogen base medium with various concentrations of Pi over 10 hours at pH 7.0 The pH indicator did not change from blue to yellow until the yeast cells had significant growth in culture. The colors of these three yeasts containing wild-type and Δpho84 with and without OsPT4 were all blue at the Pi concentration of 0 μM, revealing that little or no yeast cells had grown in medium without Pi. After increasing the Pi concentration of the medium to 75 μM, the color changed to yellow, with wild-type and Δpho84 expressing OsPT4 rather than mutant yeast, suggesting that the expression of OsPT4 restored the yeast growth rate (Fig 4A). To further confirm this result, absorbance of these different yeasts at 600 nm was tested and showed that OsPT4 could partially complement the function of yeast Pi transporter PHO84 (Fig 4B).

Bottom Line: Overexpressing OsPT4 resulted in significant higher Pi accumulation in roots, straw and brown rice, and suppression of OsPT4 caused decreased Pi concentration in straw and brown rice.The transcript level of OsPT4 increased significantly both in shoots and roots with a long time Pi starvation.We concluded that OsPT4 is a functional Pi-influx transporter involved in Pi absorption in rice that might play a role in Pi translocation.

View Article: PubMed Central - PubMed

Affiliation: National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan, 430070, P.R. China.

ABSTRACT
A total of 13 phosphate transporters in rice (Oryza sative) have been identified as belonging to the Pht1 family, which mediates inorganic phosphate (Pi) uptake and transport. We report the biological property and physiological role of OsPht1;4 (OsPT4). Overexpressing OsPT4 resulted in significant higher Pi accumulation in roots, straw and brown rice, and suppression of OsPT4 caused decreased Pi concentration in straw and brown rice. Expression of the β-glucuronidase reporter gene driven by the OsPT4 promoter showed that OsPT4 is expressed in roots, leaves, ligules, stamens, and caryopses under sufficient Pi conditions, consistent with the expression profile showing that OsPT4 has high expression in roots and flag leaves. The transcript level of OsPT4 increased significantly both in shoots and roots with a long time Pi starvation. OsPT4 encoded a plasma membrane-localized protein and was able to complement the function of the Pi transporter gene PHO84 in yeast. We concluded that OsPT4 is a functional Pi-influx transporter involved in Pi absorption in rice that might play a role in Pi translocation. This study will enrich our understanding about the physiological function of rice Pht1 family genes.

No MeSH data available.


Related in: MedlinePlus