Limits...
Confirmation of immunoglobulin heavy chain rearrangement by polymerase chain reaction using surgically obtained, paraffin-embedded samples to diagnose primary palate mucosa-associated lymphoid tissue lymphoma: A case study.

Abe S, Yokomizo N, Kobayashi Y, Yamamoto K - Int J Surg Case Rep (2015)

Bottom Line: Intraoral mucosa-associated lymphoid tissue (MALT) lymphoma is a rare lymphoma that has a good prognosis if diagnosed correctly and treated in time.Although the lesion was completely excised, histological findings did not allow a definitive diagnosis due to an absence of visible monoclonality.PCR technique is rapid, accurate, and enables a definitive diagnosis without relying on traditional histological or molecular diagnostic techniques, such as Southern blotting.

View Article: PubMed Central - PubMed

Affiliation: Department of Dentistry and Oral Surgery, Tokyo Metropolitan Hiroo Hospital, Tokyo, Japan. Electronic address: shigehiroabe@hotmail.com.

No MeSH data available.


Related in: MedlinePlus

PCR analysis of IgH rearrangements. The PCR product showed monoclonal peaks of VH(FR1)/JH, VH(FR2)/JH, and VH(FR3)/JH IgH gene rearrangement on capillary electrophoresis.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4430206&req=5

fig0015: PCR analysis of IgH rearrangements. The PCR product showed monoclonal peaks of VH(FR1)/JH, VH(FR2)/JH, and VH(FR3)/JH IgH gene rearrangement on capillary electrophoresis.

Mentions: Finally, we ordered a molecular analysis of the immunoglobulin heavy chains using the FFPE sections of a postoperative sample. We performed PCR using extracted DNA from the materials and observed a monoclonal peak of VH(FR1)/JH, VH(FR2)/JH, and VH(FR3)/JH immunoglobulin heavy chain (IgH) gene rearrangement on capillary electrophoresis (Fig. 3). The final diagnosis was MALT lymphoma of the hard palate.


Confirmation of immunoglobulin heavy chain rearrangement by polymerase chain reaction using surgically obtained, paraffin-embedded samples to diagnose primary palate mucosa-associated lymphoid tissue lymphoma: A case study.

Abe S, Yokomizo N, Kobayashi Y, Yamamoto K - Int J Surg Case Rep (2015)

PCR analysis of IgH rearrangements. The PCR product showed monoclonal peaks of VH(FR1)/JH, VH(FR2)/JH, and VH(FR3)/JH IgH gene rearrangement on capillary electrophoresis.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4430206&req=5

fig0015: PCR analysis of IgH rearrangements. The PCR product showed monoclonal peaks of VH(FR1)/JH, VH(FR2)/JH, and VH(FR3)/JH IgH gene rearrangement on capillary electrophoresis.
Mentions: Finally, we ordered a molecular analysis of the immunoglobulin heavy chains using the FFPE sections of a postoperative sample. We performed PCR using extracted DNA from the materials and observed a monoclonal peak of VH(FR1)/JH, VH(FR2)/JH, and VH(FR3)/JH immunoglobulin heavy chain (IgH) gene rearrangement on capillary electrophoresis (Fig. 3). The final diagnosis was MALT lymphoma of the hard palate.

Bottom Line: Intraoral mucosa-associated lymphoid tissue (MALT) lymphoma is a rare lymphoma that has a good prognosis if diagnosed correctly and treated in time.Although the lesion was completely excised, histological findings did not allow a definitive diagnosis due to an absence of visible monoclonality.PCR technique is rapid, accurate, and enables a definitive diagnosis without relying on traditional histological or molecular diagnostic techniques, such as Southern blotting.

View Article: PubMed Central - PubMed

Affiliation: Department of Dentistry and Oral Surgery, Tokyo Metropolitan Hiroo Hospital, Tokyo, Japan. Electronic address: shigehiroabe@hotmail.com.

No MeSH data available.


Related in: MedlinePlus