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Confirmation of immunoglobulin heavy chain rearrangement by polymerase chain reaction using surgically obtained, paraffin-embedded samples to diagnose primary palate mucosa-associated lymphoid tissue lymphoma: A case study.

Abe S, Yokomizo N, Kobayashi Y, Yamamoto K - Int J Surg Case Rep (2015)

Bottom Line: Intraoral mucosa-associated lymphoid tissue (MALT) lymphoma is a rare lymphoma that has a good prognosis if diagnosed correctly and treated in time.Although the lesion was completely excised, histological findings did not allow a definitive diagnosis due to an absence of visible monoclonality.PCR technique is rapid, accurate, and enables a definitive diagnosis without relying on traditional histological or molecular diagnostic techniques, such as Southern blotting.

View Article: PubMed Central - PubMed

Affiliation: Department of Dentistry and Oral Surgery, Tokyo Metropolitan Hiroo Hospital, Tokyo, Japan. Electronic address: shigehiroabe@hotmail.com.

No MeSH data available.


Related in: MedlinePlus

Case histopathology. (a and b) Post-excision clinical photograph of the tumor. The arrow indicates an intraoperative biopsy on the oral side (a) and deep side (b). (c and d) Hematoxylin-eosin staining. The lymphocytic infiltrate is observed between the lymphoid follicles, showing hyperplasia from the germinal center to stroma deep to an oral squamous epithelium (c). High-power magnification shows a tumor composed of centrocyte-like cells with small lymphocyte-like cell infiltrates (d). (e and f) Tumor immunohistochemistry: CD20 (positive) (e) and CD3 (negative; positive at normal T-lymphocytes) (f). Original magnification (c) ×40, (d–f) ×400.
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fig0010: Case histopathology. (a and b) Post-excision clinical photograph of the tumor. The arrow indicates an intraoperative biopsy on the oral side (a) and deep side (b). (c and d) Hematoxylin-eosin staining. The lymphocytic infiltrate is observed between the lymphoid follicles, showing hyperplasia from the germinal center to stroma deep to an oral squamous epithelium (c). High-power magnification shows a tumor composed of centrocyte-like cells with small lymphocyte-like cell infiltrates (d). (e and f) Tumor immunohistochemistry: CD20 (positive) (e) and CD3 (negative; positive at normal T-lymphocytes) (f). Original magnification (c) ×40, (d–f) ×400.

Mentions: The intraoperative rapid diagnosis of the lesion strongly suggested MALT lymphoma. The tumor was resected with an adequate safety margin of 5 mm and the palatal bone touching the tumor was shaved off along with the bone bar (Fig. 2a, b). The palatal mucosal defect was reconstructed using artificial dermis (Terudermis®). At a follow-up examination 9 months later, no recurrence or metastasis was seen (data not shown). An upper gastrointestinal endoscopy failed to detect Helicobacter pylori or any other abnormal endoscopic findings.


Confirmation of immunoglobulin heavy chain rearrangement by polymerase chain reaction using surgically obtained, paraffin-embedded samples to diagnose primary palate mucosa-associated lymphoid tissue lymphoma: A case study.

Abe S, Yokomizo N, Kobayashi Y, Yamamoto K - Int J Surg Case Rep (2015)

Case histopathology. (a and b) Post-excision clinical photograph of the tumor. The arrow indicates an intraoperative biopsy on the oral side (a) and deep side (b). (c and d) Hematoxylin-eosin staining. The lymphocytic infiltrate is observed between the lymphoid follicles, showing hyperplasia from the germinal center to stroma deep to an oral squamous epithelium (c). High-power magnification shows a tumor composed of centrocyte-like cells with small lymphocyte-like cell infiltrates (d). (e and f) Tumor immunohistochemistry: CD20 (positive) (e) and CD3 (negative; positive at normal T-lymphocytes) (f). Original magnification (c) ×40, (d–f) ×400.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4430206&req=5

fig0010: Case histopathology. (a and b) Post-excision clinical photograph of the tumor. The arrow indicates an intraoperative biopsy on the oral side (a) and deep side (b). (c and d) Hematoxylin-eosin staining. The lymphocytic infiltrate is observed between the lymphoid follicles, showing hyperplasia from the germinal center to stroma deep to an oral squamous epithelium (c). High-power magnification shows a tumor composed of centrocyte-like cells with small lymphocyte-like cell infiltrates (d). (e and f) Tumor immunohistochemistry: CD20 (positive) (e) and CD3 (negative; positive at normal T-lymphocytes) (f). Original magnification (c) ×40, (d–f) ×400.
Mentions: The intraoperative rapid diagnosis of the lesion strongly suggested MALT lymphoma. The tumor was resected with an adequate safety margin of 5 mm and the palatal bone touching the tumor was shaved off along with the bone bar (Fig. 2a, b). The palatal mucosal defect was reconstructed using artificial dermis (Terudermis®). At a follow-up examination 9 months later, no recurrence or metastasis was seen (data not shown). An upper gastrointestinal endoscopy failed to detect Helicobacter pylori or any other abnormal endoscopic findings.

Bottom Line: Intraoral mucosa-associated lymphoid tissue (MALT) lymphoma is a rare lymphoma that has a good prognosis if diagnosed correctly and treated in time.Although the lesion was completely excised, histological findings did not allow a definitive diagnosis due to an absence of visible monoclonality.PCR technique is rapid, accurate, and enables a definitive diagnosis without relying on traditional histological or molecular diagnostic techniques, such as Southern blotting.

View Article: PubMed Central - PubMed

Affiliation: Department of Dentistry and Oral Surgery, Tokyo Metropolitan Hiroo Hospital, Tokyo, Japan. Electronic address: shigehiroabe@hotmail.com.

No MeSH data available.


Related in: MedlinePlus