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Serum retinol binding protein 4 is associated with visceral fat in human with nonalcoholic fatty liver disease without known diabetes: a cross-sectional study.

Chang X, Yan H, Bian H, Xia M, Zhang L, Gao J, Gao X - Lipids Health Dis (2015)

Bottom Line: High serum Retinol Binding Protein 4 (RBP4) levels were associated with insulin-resistant states in humans.Circulating RBP4 was negatively associated with high-density lipoprotein cholesterol (HDL-c) (r=-0.392, p<0.001), but positively with waist-to-hip ratio (WHR) (r=0.343, p=0.001), triglyceride (r=0.330, p=0.002), VFA (r=0.298, p=0.027), systolic blood pressure (r=0.247, p=0.020), diastolic blood pressure (r=0.241, p=0.023), γ-GT (r=0.239, p=0.034), waist circumference (r=0.218, p=0.040).Differently, serum RBP4 levels were not associated with HFC (r=0.199, p=0.071), SFA, age, BMI, total cholesterol, low-density lipoprotein cholesterol (LDL-c), ALT or AST (all p>0.05).

View Article: PubMed Central - PubMed

Affiliation: Department of Endocrinology and Metabolism, Zhongshan Hospital, Fudan University, Shanghai, 200032, China. changxinxiawei@163.com.

ABSTRACT

Background: High serum Retinol Binding Protein 4 (RBP4) levels were associated with insulin-resistant states in humans. To determine which fat compartments are associated with elevated RBP4 levels in humans, we measured serum RBP4 and hepatic fat content (HFC), visceral (VFA) and subcutaneous abdominal fat area (SFA) in 106 subjects with non-alcoholic fatty liver disease (NAFLD) without known diabetes.

Methods: 106 patients with NAFLD (M/F: 61/45, aged 47.44±14.16 years) were enrolled. Subjects with known diabetes, chronic virus hepatitis, and those with alcohol consumption≥30 g/d in man and ≥20 g/d in woman were excluded. Anthropometrics and laboratory tests, including lipid profile, alanine aminotransferase (ALT), aspartate aminotransferase (AST) and γ-glutamyltransferase (γ-GT) were conducted. HFC, VFA and SFA were determined by CT scan. Serum RBP4 was detected by an enzyme immunoassay kit and validated by quantitative Western blotting.

Results: Circulating RBP4 was negatively associated with high-density lipoprotein cholesterol (HDL-c) (r=-0.392, p<0.001), but positively with waist-to-hip ratio (WHR) (r=0.343, p=0.001), triglyceride (r=0.330, p=0.002), VFA (r=0.298, p=0.027), systolic blood pressure (r=0.247, p=0.020), diastolic blood pressure (r=0.241, p=0.023), γ-GT (r=0.239, p=0.034), waist circumference (r=0.218, p=0.040). Differently, serum RBP4 levels were not associated with HFC (r=0.199, p=0.071), SFA, age, BMI, total cholesterol, low-density lipoprotein cholesterol (LDL-c), ALT or AST (all p>0.05). Multiple linear regression analysis revealed that RBP4 correlated independently with VFA (Standard β=0.357, p=0.019) and HDL-c (Standard β=-0.345, p=0.023) in all subjects, HDL-c (Standard β=-0.315, p=0.040) in men, VFA/SFA in women (Standard β=0.471, p=0.049), not with HFC. However, serum RBP4 was positively correlated with HFC when HFC below 6.34% (r=0.574, p=0.001).

Conclusions: RBP4 could be a marker of abdominal obesity, however, the role of RBP4 in the pathogenesis of NAFLD is not sufficiently elucidated.

No MeSH data available.


Related in: MedlinePlus

The association between serum RBP4 level and the quartiles of hepatic fat content. When hepatic fat content increased by quartile order, serum RBP4 level was 43.23 ± 1.19, 54.00 ± 8.74, 51.75 ± 1.52, 54.68 ± 8.86 μg/ml respectively. Serum RBP4 is not proportionally increased with hepatic fat content (p = 0.298).
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Fig1: The association between serum RBP4 level and the quartiles of hepatic fat content. When hepatic fat content increased by quartile order, serum RBP4 level was 43.23 ± 1.19, 54.00 ± 8.74, 51.75 ± 1.52, 54.68 ± 8.86 μg/ml respectively. Serum RBP4 is not proportionally increased with hepatic fat content (p = 0.298).

Mentions: The RBP4 concentrations were 43.23 ± 1.19 μg/ml, 54.00 ± 8.74 μg/ml, 51.75 ± 1.52 μg/ml and 54.68 ± 8.86 μg/ml when HFC increased by quartile order, respectively. Although the difference of RBP4 among the four groups was not statistically significant, we found a phenomenon that serum RBP4 level increased when HFC increased from Q1 to Q2, but, it did not increased anymore when HFC increased from Q2 to Q4 (p = 0.298, Figure 1, Table 1). So, we predicted that there might be a correlation between RBP4 and HFC when HFC was mildly elevated (less than the median of 6.34%).Figure 1


Serum retinol binding protein 4 is associated with visceral fat in human with nonalcoholic fatty liver disease without known diabetes: a cross-sectional study.

Chang X, Yan H, Bian H, Xia M, Zhang L, Gao J, Gao X - Lipids Health Dis (2015)

The association between serum RBP4 level and the quartiles of hepatic fat content. When hepatic fat content increased by quartile order, serum RBP4 level was 43.23 ± 1.19, 54.00 ± 8.74, 51.75 ± 1.52, 54.68 ± 8.86 μg/ml respectively. Serum RBP4 is not proportionally increased with hepatic fat content (p = 0.298).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4419494&req=5

Fig1: The association between serum RBP4 level and the quartiles of hepatic fat content. When hepatic fat content increased by quartile order, serum RBP4 level was 43.23 ± 1.19, 54.00 ± 8.74, 51.75 ± 1.52, 54.68 ± 8.86 μg/ml respectively. Serum RBP4 is not proportionally increased with hepatic fat content (p = 0.298).
Mentions: The RBP4 concentrations were 43.23 ± 1.19 μg/ml, 54.00 ± 8.74 μg/ml, 51.75 ± 1.52 μg/ml and 54.68 ± 8.86 μg/ml when HFC increased by quartile order, respectively. Although the difference of RBP4 among the four groups was not statistically significant, we found a phenomenon that serum RBP4 level increased when HFC increased from Q1 to Q2, but, it did not increased anymore when HFC increased from Q2 to Q4 (p = 0.298, Figure 1, Table 1). So, we predicted that there might be a correlation between RBP4 and HFC when HFC was mildly elevated (less than the median of 6.34%).Figure 1

Bottom Line: High serum Retinol Binding Protein 4 (RBP4) levels were associated with insulin-resistant states in humans.Circulating RBP4 was negatively associated with high-density lipoprotein cholesterol (HDL-c) (r=-0.392, p<0.001), but positively with waist-to-hip ratio (WHR) (r=0.343, p=0.001), triglyceride (r=0.330, p=0.002), VFA (r=0.298, p=0.027), systolic blood pressure (r=0.247, p=0.020), diastolic blood pressure (r=0.241, p=0.023), γ-GT (r=0.239, p=0.034), waist circumference (r=0.218, p=0.040).Differently, serum RBP4 levels were not associated with HFC (r=0.199, p=0.071), SFA, age, BMI, total cholesterol, low-density lipoprotein cholesterol (LDL-c), ALT or AST (all p>0.05).

View Article: PubMed Central - PubMed

Affiliation: Department of Endocrinology and Metabolism, Zhongshan Hospital, Fudan University, Shanghai, 200032, China. changxinxiawei@163.com.

ABSTRACT

Background: High serum Retinol Binding Protein 4 (RBP4) levels were associated with insulin-resistant states in humans. To determine which fat compartments are associated with elevated RBP4 levels in humans, we measured serum RBP4 and hepatic fat content (HFC), visceral (VFA) and subcutaneous abdominal fat area (SFA) in 106 subjects with non-alcoholic fatty liver disease (NAFLD) without known diabetes.

Methods: 106 patients with NAFLD (M/F: 61/45, aged 47.44±14.16 years) were enrolled. Subjects with known diabetes, chronic virus hepatitis, and those with alcohol consumption≥30 g/d in man and ≥20 g/d in woman were excluded. Anthropometrics and laboratory tests, including lipid profile, alanine aminotransferase (ALT), aspartate aminotransferase (AST) and γ-glutamyltransferase (γ-GT) were conducted. HFC, VFA and SFA were determined by CT scan. Serum RBP4 was detected by an enzyme immunoassay kit and validated by quantitative Western blotting.

Results: Circulating RBP4 was negatively associated with high-density lipoprotein cholesterol (HDL-c) (r=-0.392, p<0.001), but positively with waist-to-hip ratio (WHR) (r=0.343, p=0.001), triglyceride (r=0.330, p=0.002), VFA (r=0.298, p=0.027), systolic blood pressure (r=0.247, p=0.020), diastolic blood pressure (r=0.241, p=0.023), γ-GT (r=0.239, p=0.034), waist circumference (r=0.218, p=0.040). Differently, serum RBP4 levels were not associated with HFC (r=0.199, p=0.071), SFA, age, BMI, total cholesterol, low-density lipoprotein cholesterol (LDL-c), ALT or AST (all p>0.05). Multiple linear regression analysis revealed that RBP4 correlated independently with VFA (Standard β=0.357, p=0.019) and HDL-c (Standard β=-0.345, p=0.023) in all subjects, HDL-c (Standard β=-0.315, p=0.040) in men, VFA/SFA in women (Standard β=0.471, p=0.049), not with HFC. However, serum RBP4 was positively correlated with HFC when HFC below 6.34% (r=0.574, p=0.001).

Conclusions: RBP4 could be a marker of abdominal obesity, however, the role of RBP4 in the pathogenesis of NAFLD is not sufficiently elucidated.

No MeSH data available.


Related in: MedlinePlus