Quantitative analysis of tissue deformation dynamics reveals three characteristic growth modes and globally aligned anisotropic tissue deformation during chick limb development.
Bottom Line: We also found anisotropic tissue deformation along the proximal-distal axis.Morphogenetic simulation and experimental studies suggested that this directional tissue elongation, and not local growth, has the greatest impact on limb shaping.Our study marks a pivotal point for multi-scale system understanding in vertebrate development.
Affiliation: Laboratory for Developmental Morphogeometry, RIKEN Quantitative Biology Center, Kobe 650-0047, Japan RIKEN Center for Developmental Biology, Kobe 650-0047, Japan email@example.com firstname.lastname@example.org.Show MeSH
Related in: MedlinePlus
Mentions: We recently developed a novel method that combines snapshot lineage tracing with Bayesian statistical estimation to construct whole-organ deformation maps from limited space-time point data (Morishita and Suzuki, 2014). In this method, a regular lattice enveloping the target organ before deformation is considered, and how the lattice deforms over a given time interval is estimated. Except for positional data for landmarks before and after deformation, all that is required is the biologically plausible assumption that deformation of tissue occurs smoothly. As shown below, applying the method to data for chick limb development, we here estimated the deformation map with high precision and analyzed the deformation dynamics (Figs 2, 3 and 7).Fig. 2.
Affiliation: Laboratory for Developmental Morphogeometry, RIKEN Quantitative Biology Center, Kobe 650-0047, Japan RIKEN Center for Developmental Biology, Kobe 650-0047, Japan email@example.com firstname.lastname@example.org.