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A low-noise, modular, and versatile analog front-end intended for processing in vitro neuronal signals detected by microelectrode arrays.

Regalia G, Biffi E, Ferrigno G, Pedrocchi A - Comput Intell Neurosci (2015)

Bottom Line: To address this issue, we developed a custom MEA interfacing system featuring low noise, low power, and the capability to be readily integrated inside an incubator-like environment.Two stages, a preamplifier and a filter amplifier, were designed, implemented on printed circuit boards, and tested.The system is characterized by a low input-referred noise (<1 μV RMS), a high channel separation (>70 dB), and signal-to-noise ratio values of neuronal recordings comparable to those obtained with the benchmark commercial MEA system.

View Article: PubMed Central - PubMed

Affiliation: Neuroengineering and Medical Robotics Laboratory, Electronics, Information and Bioengineering Department, Politecnico di Milano, 20133 Milan, Italy.

ABSTRACT
The collection of good quality extracellular neuronal spikes from neuronal cultures coupled to Microelectrode Arrays (MEAs) is a binding requirement to gather reliable data. Due to physical constraints, low power requirement, or the need of customizability, commercial recording platforms are not fully adequate for the development of experimental setups integrating MEA technology with other equipment needed to perform experiments under climate controlled conditions, like environmental chambers or cell culture incubators. To address this issue, we developed a custom MEA interfacing system featuring low noise, low power, and the capability to be readily integrated inside an incubator-like environment. Two stages, a preamplifier and a filter amplifier, were designed, implemented on printed circuit boards, and tested. The system is characterized by a low input-referred noise (<1 μV RMS), a high channel separation (>70 dB), and signal-to-noise ratio values of neuronal recordings comparable to those obtained with the benchmark commercial MEA system. In addition, the system was successfully integrated with an environmental MEA chamber, without harming cell cultures during experiments and without being damaged by the high humidity level. The devised system is of practical value in the development of in vitro platforms to study temporally extended neuronal network dynamics by means of MEAs.

No MeSH data available.


Related in: MedlinePlus

Example of signals detected by the same microelectrodes of a MEA recorded with the custom boards (gray (a)) and with the commercial benchmark system (black (b)).
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fig8: Example of signals detected by the same microelectrodes of a MEA recorded with the custom boards (gray (a)) and with the commercial benchmark system (black (b)).

Mentions: With the realized boards, it was possible to record extracellular signals from hippocampal cultures satisfactorily comparable with those recorded by the benchmark MEA system. An example of this is provided in Figure 8, where the spiking signals sensed by the same microelectrodes in the custom setup (gray) or in the commercial one (black) are reported. Differences in background noise levels and spike amplitudes at different sites are matched in the two setups.


A low-noise, modular, and versatile analog front-end intended for processing in vitro neuronal signals detected by microelectrode arrays.

Regalia G, Biffi E, Ferrigno G, Pedrocchi A - Comput Intell Neurosci (2015)

Example of signals detected by the same microelectrodes of a MEA recorded with the custom boards (gray (a)) and with the commercial benchmark system (black (b)).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4419262&req=5

fig8: Example of signals detected by the same microelectrodes of a MEA recorded with the custom boards (gray (a)) and with the commercial benchmark system (black (b)).
Mentions: With the realized boards, it was possible to record extracellular signals from hippocampal cultures satisfactorily comparable with those recorded by the benchmark MEA system. An example of this is provided in Figure 8, where the spiking signals sensed by the same microelectrodes in the custom setup (gray) or in the commercial one (black) are reported. Differences in background noise levels and spike amplitudes at different sites are matched in the two setups.

Bottom Line: To address this issue, we developed a custom MEA interfacing system featuring low noise, low power, and the capability to be readily integrated inside an incubator-like environment.Two stages, a preamplifier and a filter amplifier, were designed, implemented on printed circuit boards, and tested.The system is characterized by a low input-referred noise (<1 μV RMS), a high channel separation (>70 dB), and signal-to-noise ratio values of neuronal recordings comparable to those obtained with the benchmark commercial MEA system.

View Article: PubMed Central - PubMed

Affiliation: Neuroengineering and Medical Robotics Laboratory, Electronics, Information and Bioengineering Department, Politecnico di Milano, 20133 Milan, Italy.

ABSTRACT
The collection of good quality extracellular neuronal spikes from neuronal cultures coupled to Microelectrode Arrays (MEAs) is a binding requirement to gather reliable data. Due to physical constraints, low power requirement, or the need of customizability, commercial recording platforms are not fully adequate for the development of experimental setups integrating MEA technology with other equipment needed to perform experiments under climate controlled conditions, like environmental chambers or cell culture incubators. To address this issue, we developed a custom MEA interfacing system featuring low noise, low power, and the capability to be readily integrated inside an incubator-like environment. Two stages, a preamplifier and a filter amplifier, were designed, implemented on printed circuit boards, and tested. The system is characterized by a low input-referred noise (<1 μV RMS), a high channel separation (>70 dB), and signal-to-noise ratio values of neuronal recordings comparable to those obtained with the benchmark commercial MEA system. In addition, the system was successfully integrated with an environmental MEA chamber, without harming cell cultures during experiments and without being damaged by the high humidity level. The devised system is of practical value in the development of in vitro platforms to study temporally extended neuronal network dynamics by means of MEAs.

No MeSH data available.


Related in: MedlinePlus