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Enhancing Phenolic Contents and Antioxidant Potentials of Antidesma thwaitesianum by Supercritical Carbon Dioxide Extraction.

Poontawee W, Natakankitkul S, Wongmekiat O - J Anal Methods Chem (2015)

Bottom Line: Arg. (Phyllanthaceae), or ma mao, has been reported to exhibit antioxidant health benefits due to its phenolic constituents.The results showed that the SC-CO2 extract contained significantly higher yield, total phenolic, flavonoid, and proanthocyanidin contents than those obtained from ethanol and water.The findings added more reliability of using this technique to produce high added value products from this medicinal plant.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand.

ABSTRACT
Supercritical fluid extraction (SFE) has increasingly gained attention as an alternative technique for extraction of natural products without leaving toxic residues in extracts. Antidesma thwaitesianum Muell. Arg. (Phyllanthaceae), or ma mao, has been reported to exhibit antioxidant health benefits due to its phenolic constituents. To determine whether SFE technique could impact on phenolic contents and associated antioxidant potentials, ripe fruits of Antidesma thwaitesianum (Phyllanthaceae) were extracted using supercritical carbon dioxide (SC-CO2) and conventional solvents (ethanol, water). The results showed that the SC-CO2 extract contained significantly higher yield, total phenolic, flavonoid, and proanthocyanidin contents than those obtained from ethanol and water. It also demonstrated the greatest antioxidant activities as assessed by ABTS radical cation decolorization, DPPH radical scavenging, and ferric reducing antioxidant power (FRAP) assays. Further analysis using high-performance liquid chromatography with diode array and mass spectrometry detectors (HPLC-DAD/MSD) revealed the presence of catechin as a major phenolic compound of Antidesma thwaitesianum (Phyllanthaceae), with the maximum amount detected in the SC-CO2 extract. These data indicate that SFE technology improves both quantity and quality of Antidesma thwaitesianum fruit extract. The findings added more reliability of using this technique to produce high added value products from this medicinal plant.

No MeSH data available.


Related in: MedlinePlus

Effects of SC-CO2 extraction on antioxidant activity of Antidesma thwaitesianum as assessed by (a) ABTS, (b) DPPH, and (c) FRAP. Values are mean ± SD (n = 3). TE, trolox equivalent. Means with different letters (A–C) are significantly different (P < 0.05).
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Related In: Results  -  Collection


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fig2: Effects of SC-CO2 extraction on antioxidant activity of Antidesma thwaitesianum as assessed by (a) ABTS, (b) DPPH, and (c) FRAP. Values are mean ± SD (n = 3). TE, trolox equivalent. Means with different letters (A–C) are significantly different (P < 0.05).

Mentions: Because of the complex nature of polyphenols, the antioxidant activities of plant extracts cannot be evaluated by only a single method. In this study, the ABTS, DPPH, and FRAP assays were used to determine the antioxidant potentials of Antidesma thwaitesianum extracts and the results are shown in Figure 2. The data clearly demonstrated the significantly greater (P < 0.05) scavenging activities of the extract from SC-CO2 against ABTS and DPPH radicals than those of the ethanol and water. Ferric reducing antioxidant power also showed similar profile as the radical scavenging activities in which the greatest ability (P < 0.05) was detectable from the SC-CO2 extract.


Enhancing Phenolic Contents and Antioxidant Potentials of Antidesma thwaitesianum by Supercritical Carbon Dioxide Extraction.

Poontawee W, Natakankitkul S, Wongmekiat O - J Anal Methods Chem (2015)

Effects of SC-CO2 extraction on antioxidant activity of Antidesma thwaitesianum as assessed by (a) ABTS, (b) DPPH, and (c) FRAP. Values are mean ± SD (n = 3). TE, trolox equivalent. Means with different letters (A–C) are significantly different (P < 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4419227&req=5

fig2: Effects of SC-CO2 extraction on antioxidant activity of Antidesma thwaitesianum as assessed by (a) ABTS, (b) DPPH, and (c) FRAP. Values are mean ± SD (n = 3). TE, trolox equivalent. Means with different letters (A–C) are significantly different (P < 0.05).
Mentions: Because of the complex nature of polyphenols, the antioxidant activities of plant extracts cannot be evaluated by only a single method. In this study, the ABTS, DPPH, and FRAP assays were used to determine the antioxidant potentials of Antidesma thwaitesianum extracts and the results are shown in Figure 2. The data clearly demonstrated the significantly greater (P < 0.05) scavenging activities of the extract from SC-CO2 against ABTS and DPPH radicals than those of the ethanol and water. Ferric reducing antioxidant power also showed similar profile as the radical scavenging activities in which the greatest ability (P < 0.05) was detectable from the SC-CO2 extract.

Bottom Line: Arg. (Phyllanthaceae), or ma mao, has been reported to exhibit antioxidant health benefits due to its phenolic constituents.The results showed that the SC-CO2 extract contained significantly higher yield, total phenolic, flavonoid, and proanthocyanidin contents than those obtained from ethanol and water.The findings added more reliability of using this technique to produce high added value products from this medicinal plant.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand.

ABSTRACT
Supercritical fluid extraction (SFE) has increasingly gained attention as an alternative technique for extraction of natural products without leaving toxic residues in extracts. Antidesma thwaitesianum Muell. Arg. (Phyllanthaceae), or ma mao, has been reported to exhibit antioxidant health benefits due to its phenolic constituents. To determine whether SFE technique could impact on phenolic contents and associated antioxidant potentials, ripe fruits of Antidesma thwaitesianum (Phyllanthaceae) were extracted using supercritical carbon dioxide (SC-CO2) and conventional solvents (ethanol, water). The results showed that the SC-CO2 extract contained significantly higher yield, total phenolic, flavonoid, and proanthocyanidin contents than those obtained from ethanol and water. It also demonstrated the greatest antioxidant activities as assessed by ABTS radical cation decolorization, DPPH radical scavenging, and ferric reducing antioxidant power (FRAP) assays. Further analysis using high-performance liquid chromatography with diode array and mass spectrometry detectors (HPLC-DAD/MSD) revealed the presence of catechin as a major phenolic compound of Antidesma thwaitesianum (Phyllanthaceae), with the maximum amount detected in the SC-CO2 extract. These data indicate that SFE technology improves both quantity and quality of Antidesma thwaitesianum fruit extract. The findings added more reliability of using this technique to produce high added value products from this medicinal plant.

No MeSH data available.


Related in: MedlinePlus