Limits...
Peripheral vagus nerve stimulation significantly affects lipid composition and protein secondary structure within dopamine-related brain regions in rats.

Surowka AD, Krygowska-Wajs A, Ziomber A, Thor P, Chrobak AA, Szczerbowska-Boruchowska M - Neuromolecular Med. (2015)

Bottom Line: It was found that the chronic vagal nerve stimulation strongly affects the chain length of fatty acids within the ventral tegmental area, nucleus accumbens, substantia nigra, striatum, dorsal motor nucleus of vagus and the motor cortex.In particular, the level of lipid unsaturation was found significantly increasing in the ventral tegmental area, substantia nigra and motor cortex as a result of vagal nerve stimulation.This is due to the co-occurrence of statistically significant changes in the content of non-ordered structure components, alpha helices, beta sheets, and the total area of Amide I.

View Article: PubMed Central - PubMed

Affiliation: AGH University of Science and Technology, Faculty of Physics and Applied Computer Science, Al. Mickiewicza 30, 30-059, Kraków, Poland, asurowka@agh.edu.pl.

ABSTRACT
Recent immunohistochemical studies point to the dorsal motor nucleus of the vagus nerve as the point of departure of initial changes which are related to the gradual pathological developments in the dopaminergic system. In the light of current investigations, it is likely that biochemical changes within the peripheral nervous system may influence the physiology of the dopaminergic system, suggesting a putative role for it in the development of neurodegenerative disorders. By using Fourier transform infrared microspectroscopy, coupled with statistical analysis, we examined the effect of chronic, unilateral electrical vagus nerve stimulation on changes in lipid composition and in protein secondary structure within dopamine-related brain structures in rats. It was found that the chronic vagal nerve stimulation strongly affects the chain length of fatty acids within the ventral tegmental area, nucleus accumbens, substantia nigra, striatum, dorsal motor nucleus of vagus and the motor cortex. In particular, the level of lipid unsaturation was found significantly increasing in the ventral tegmental area, substantia nigra and motor cortex as a result of vagal nerve stimulation. When it comes to changes in protein secondary structure, we could see that the mesolimbic, mesocortical and nigrostriatal dopaminergic pathways are particularly affected by vagus nerve stimulation. This is due to the co-occurrence of statistically significant changes in the content of non-ordered structure components, alpha helices, beta sheets, and the total area of Amide I. Macromolecular changes caused by peripheral vagus nerve stimulation may highlight a potential connection between the gastrointestinal system and the central nervous system in rat during the development of neurodegenerative disorders.

No MeSH data available.


Related in: MedlinePlus

Global spectral changes in Amide I for: a dorsal motor nucleus of the vagus nerve (DMV); b ventral tegmental area (VTA); c substantia nigra (SN); d nucleus accumbens (NAC); e corpus striatum spaces (STR_E); f fiber bundles of striatum (STR_F); g motor cortex (CX). CR—control group, right hemisphere; CL—control group, left hemisphere; MCR—microchip-stimulated group, right hemisphere; MCL—microchip-stimulated group, left hemisphere
© Copyright Policy - OpenAccess
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4419184&req=5

Fig9: Global spectral changes in Amide I for: a dorsal motor nucleus of the vagus nerve (DMV); b ventral tegmental area (VTA); c substantia nigra (SN); d nucleus accumbens (NAC); e corpus striatum spaces (STR_E); f fiber bundles of striatum (STR_F); g motor cortex (CX). CR—control group, right hemisphere; CL—control group, left hemisphere; MCR—microchip-stimulated group, right hemisphere; MCL—microchip-stimulated group, left hemisphere

Mentions: Average FTIRMS spectra, shown in Figs. 8 and 9 for C–H str. and Amide I–II spectral range, respectively, were calculated for each structure analyzed, based on all the spectra taken into account. By analyzing Fig. 8, it is apparent that the secondary structure of proteins was markedly changed in both VTA and SN of the left hemisphere upon electric stimulation, as their Amide I for MC group was markedly broadened, decreased and shifted toward lower wavenumbers. The most striking observation which emerges from the data shown in Fig. 9 is the shift of the olefinic band (3012 cm−1) toward higher wavenumbers for all brain structures analyzed in MC group compared with control. Noteworthy, in MC group, the mean FTIRMS profile of VTA of the left hemisphere revealed a dramatic decrease in the intensity of vibration modes arising from of C–H str.Fig. 8


Peripheral vagus nerve stimulation significantly affects lipid composition and protein secondary structure within dopamine-related brain regions in rats.

Surowka AD, Krygowska-Wajs A, Ziomber A, Thor P, Chrobak AA, Szczerbowska-Boruchowska M - Neuromolecular Med. (2015)

Global spectral changes in Amide I for: a dorsal motor nucleus of the vagus nerve (DMV); b ventral tegmental area (VTA); c substantia nigra (SN); d nucleus accumbens (NAC); e corpus striatum spaces (STR_E); f fiber bundles of striatum (STR_F); g motor cortex (CX). CR—control group, right hemisphere; CL—control group, left hemisphere; MCR—microchip-stimulated group, right hemisphere; MCL—microchip-stimulated group, left hemisphere
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4419184&req=5

Fig9: Global spectral changes in Amide I for: a dorsal motor nucleus of the vagus nerve (DMV); b ventral tegmental area (VTA); c substantia nigra (SN); d nucleus accumbens (NAC); e corpus striatum spaces (STR_E); f fiber bundles of striatum (STR_F); g motor cortex (CX). CR—control group, right hemisphere; CL—control group, left hemisphere; MCR—microchip-stimulated group, right hemisphere; MCL—microchip-stimulated group, left hemisphere
Mentions: Average FTIRMS spectra, shown in Figs. 8 and 9 for C–H str. and Amide I–II spectral range, respectively, were calculated for each structure analyzed, based on all the spectra taken into account. By analyzing Fig. 8, it is apparent that the secondary structure of proteins was markedly changed in both VTA and SN of the left hemisphere upon electric stimulation, as their Amide I for MC group was markedly broadened, decreased and shifted toward lower wavenumbers. The most striking observation which emerges from the data shown in Fig. 9 is the shift of the olefinic band (3012 cm−1) toward higher wavenumbers for all brain structures analyzed in MC group compared with control. Noteworthy, in MC group, the mean FTIRMS profile of VTA of the left hemisphere revealed a dramatic decrease in the intensity of vibration modes arising from of C–H str.Fig. 8

Bottom Line: It was found that the chronic vagal nerve stimulation strongly affects the chain length of fatty acids within the ventral tegmental area, nucleus accumbens, substantia nigra, striatum, dorsal motor nucleus of vagus and the motor cortex.In particular, the level of lipid unsaturation was found significantly increasing in the ventral tegmental area, substantia nigra and motor cortex as a result of vagal nerve stimulation.This is due to the co-occurrence of statistically significant changes in the content of non-ordered structure components, alpha helices, beta sheets, and the total area of Amide I.

View Article: PubMed Central - PubMed

Affiliation: AGH University of Science and Technology, Faculty of Physics and Applied Computer Science, Al. Mickiewicza 30, 30-059, Kraków, Poland, asurowka@agh.edu.pl.

ABSTRACT
Recent immunohistochemical studies point to the dorsal motor nucleus of the vagus nerve as the point of departure of initial changes which are related to the gradual pathological developments in the dopaminergic system. In the light of current investigations, it is likely that biochemical changes within the peripheral nervous system may influence the physiology of the dopaminergic system, suggesting a putative role for it in the development of neurodegenerative disorders. By using Fourier transform infrared microspectroscopy, coupled with statistical analysis, we examined the effect of chronic, unilateral electrical vagus nerve stimulation on changes in lipid composition and in protein secondary structure within dopamine-related brain structures in rats. It was found that the chronic vagal nerve stimulation strongly affects the chain length of fatty acids within the ventral tegmental area, nucleus accumbens, substantia nigra, striatum, dorsal motor nucleus of vagus and the motor cortex. In particular, the level of lipid unsaturation was found significantly increasing in the ventral tegmental area, substantia nigra and motor cortex as a result of vagal nerve stimulation. When it comes to changes in protein secondary structure, we could see that the mesolimbic, mesocortical and nigrostriatal dopaminergic pathways are particularly affected by vagus nerve stimulation. This is due to the co-occurrence of statistically significant changes in the content of non-ordered structure components, alpha helices, beta sheets, and the total area of Amide I. Macromolecular changes caused by peripheral vagus nerve stimulation may highlight a potential connection between the gastrointestinal system and the central nervous system in rat during the development of neurodegenerative disorders.

No MeSH data available.


Related in: MedlinePlus