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Using recombinant Chlamydia trachomatis OMP2 as antigen in diagnostic ELISA test.

Javaherian M, Sharifnia Z, Taheripanah R, Bandepour M, Soleimani M, Kazemi B - Iran J Microbiol (2014)

Bottom Line: The final results of this research were compared with Euroimmun commercial kit.The sensitivity and specificity of rOMP2 in ELISA for detection of C. trachomatis were 90% and 86%, respectively.Though the sensitivity was higher than results of Euroimmun commercial kit, its specificity was calculated lower than reference kit.

View Article: PubMed Central - PubMed

Affiliation: Microbiology Department, Islamic Azad University, Qom, Iran.

ABSTRACT

Background and objectives: The obligate intracellular bacterium Chlamydia trachomatis causes sexually transmissible diseases in human. Timely and sensitive detection of this pathogen is very important. There are many cross-reactions in bacteriological and serological methods in detection of this type of pathogens. The aim of this study was to achieve a more specific antigen for serological tests.

Materials and methods: Blood samples were taken from 192 women with suspected chlamydial infection and sera were isolated. ELISA plate wells were coated with recombinant C. trachomatis OMP2 as antigen. Cut-off system was determined with 40 negative sera. The final results of this research were compared with Euroimmun commercial kit.

Results: The ELISA system cut-off was calculated at 0.27 using negative sera samples. ODs of positive samples were higher than 0.27 and negative samples were lower than it. We obtained 30 samples (15.62%) as positive and 162 cases (84.37%) as negative. Sensitivity and specificity of the recombinant antigen were 90% and 86%, respectively. This antigen showed no cross-reactivity with sera of patients infected with Hydatid cyst, HCV, Epstein barr virus, HBV, Helicobacter pylori, Toxoplasma gondii, Cytomegalovirus, Mycoplasma, Measles and Varicella zoster virus.

Conclusion: The sensitivity and specificity of rOMP2 in ELISA for detection of C. trachomatis were 90% and 86%, respectively. Though the sensitivity was higher than results of Euroimmun commercial kit, its specificity was calculated lower than reference kit.

No MeSH data available.


Related in: MedlinePlus

Comparison of sera dilutions with 3mg/mL and 2mg/mL of rOMP2 protein.
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Figure 3: Comparison of sera dilutions with 3mg/mL and 2mg/mL of rOMP2 protein.

Mentions: We tested antigen concentration for coating on microplate wells with 3 mg/ml and 2 mg/ml and used for serum concentration (Fig.3). The OD of 192 C. trachomatis suspected serum were calculated by rOMP2 and Euroimmun commercial kit as shown in Figs. 4 and 5.


Using recombinant Chlamydia trachomatis OMP2 as antigen in diagnostic ELISA test.

Javaherian M, Sharifnia Z, Taheripanah R, Bandepour M, Soleimani M, Kazemi B - Iran J Microbiol (2014)

Comparison of sera dilutions with 3mg/mL and 2mg/mL of rOMP2 protein.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4419049&req=5

Figure 3: Comparison of sera dilutions with 3mg/mL and 2mg/mL of rOMP2 protein.
Mentions: We tested antigen concentration for coating on microplate wells with 3 mg/ml and 2 mg/ml and used for serum concentration (Fig.3). The OD of 192 C. trachomatis suspected serum were calculated by rOMP2 and Euroimmun commercial kit as shown in Figs. 4 and 5.

Bottom Line: The final results of this research were compared with Euroimmun commercial kit.The sensitivity and specificity of rOMP2 in ELISA for detection of C. trachomatis were 90% and 86%, respectively.Though the sensitivity was higher than results of Euroimmun commercial kit, its specificity was calculated lower than reference kit.

View Article: PubMed Central - PubMed

Affiliation: Microbiology Department, Islamic Azad University, Qom, Iran.

ABSTRACT

Background and objectives: The obligate intracellular bacterium Chlamydia trachomatis causes sexually transmissible diseases in human. Timely and sensitive detection of this pathogen is very important. There are many cross-reactions in bacteriological and serological methods in detection of this type of pathogens. The aim of this study was to achieve a more specific antigen for serological tests.

Materials and methods: Blood samples were taken from 192 women with suspected chlamydial infection and sera were isolated. ELISA plate wells were coated with recombinant C. trachomatis OMP2 as antigen. Cut-off system was determined with 40 negative sera. The final results of this research were compared with Euroimmun commercial kit.

Results: The ELISA system cut-off was calculated at 0.27 using negative sera samples. ODs of positive samples were higher than 0.27 and negative samples were lower than it. We obtained 30 samples (15.62%) as positive and 162 cases (84.37%) as negative. Sensitivity and specificity of the recombinant antigen were 90% and 86%, respectively. This antigen showed no cross-reactivity with sera of patients infected with Hydatid cyst, HCV, Epstein barr virus, HBV, Helicobacter pylori, Toxoplasma gondii, Cytomegalovirus, Mycoplasma, Measles and Varicella zoster virus.

Conclusion: The sensitivity and specificity of rOMP2 in ELISA for detection of C. trachomatis were 90% and 86%, respectively. Though the sensitivity was higher than results of Euroimmun commercial kit, its specificity was calculated lower than reference kit.

No MeSH data available.


Related in: MedlinePlus