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Using recombinant Chlamydia trachomatis OMP2 as antigen in diagnostic ELISA test.

Javaherian M, Sharifnia Z, Taheripanah R, Bandepour M, Soleimani M, Kazemi B - Iran J Microbiol (2014)

Bottom Line: The final results of this research were compared with Euroimmun commercial kit.ODs of positive samples were higher than 0.27 and negative samples were lower than it.Though the sensitivity was higher than results of Euroimmun commercial kit, its specificity was calculated lower than reference kit.

View Article: PubMed Central - PubMed

Affiliation: Microbiology Department, Islamic Azad University, Qom, Iran.

ABSTRACT

Background and objectives: The obligate intracellular bacterium Chlamydia trachomatis causes sexually transmissible diseases in human. Timely and sensitive detection of this pathogen is very important. There are many cross-reactions in bacteriological and serological methods in detection of this type of pathogens. The aim of this study was to achieve a more specific antigen for serological tests.

Materials and methods: Blood samples were taken from 192 women with suspected chlamydial infection and sera were isolated. ELISA plate wells were coated with recombinant C. trachomatis OMP2 as antigen. Cut-off system was determined with 40 negative sera. The final results of this research were compared with Euroimmun commercial kit.

Results: The ELISA system cut-off was calculated at 0.27 using negative sera samples. ODs of positive samples were higher than 0.27 and negative samples were lower than it. We obtained 30 samples (15.62%) as positive and 162 cases (84.37%) as negative. Sensitivity and specificity of the recombinant antigen were 90% and 86%, respectively. This antigen showed no cross-reactivity with sera of patients infected with Hydatid cyst, HCV, Epstein barr virus, HBV, Helicobacter pylori, Toxoplasma gondii, Cytomegalovirus, Mycoplasma, Measles and Varicella zoster virus.

Conclusion: The sensitivity and specificity of rOMP2 in ELISA for detection of C. trachomatis were 90% and 86%, respectively. Though the sensitivity was higher than results of Euroimmun commercial kit, its specificity was calculated lower than reference kit.

No MeSH data available.


Related in: MedlinePlus

Comparison of sera dilutions with 3mg/mL and 2mg/mL of rOMP2 protein.
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Related In: Results  -  Collection


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Figure 3: Comparison of sera dilutions with 3mg/mL and 2mg/mL of rOMP2 protein.

Mentions: We tested antigen concentration for coating on microplate wells with 3 mg/ml and 2 mg/ml and used for serum concentration (Fig.3). The OD of 192 C. trachomatis suspected serum were calculated by rOMP2 and Euroimmun commercial kit as shown in Figs. 4 and 5.


Using recombinant Chlamydia trachomatis OMP2 as antigen in diagnostic ELISA test.

Javaherian M, Sharifnia Z, Taheripanah R, Bandepour M, Soleimani M, Kazemi B - Iran J Microbiol (2014)

Comparison of sera dilutions with 3mg/mL and 2mg/mL of rOMP2 protein.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4419049&req=5

Figure 3: Comparison of sera dilutions with 3mg/mL and 2mg/mL of rOMP2 protein.
Mentions: We tested antigen concentration for coating on microplate wells with 3 mg/ml and 2 mg/ml and used for serum concentration (Fig.3). The OD of 192 C. trachomatis suspected serum were calculated by rOMP2 and Euroimmun commercial kit as shown in Figs. 4 and 5.

Bottom Line: The final results of this research were compared with Euroimmun commercial kit.ODs of positive samples were higher than 0.27 and negative samples were lower than it.Though the sensitivity was higher than results of Euroimmun commercial kit, its specificity was calculated lower than reference kit.

View Article: PubMed Central - PubMed

Affiliation: Microbiology Department, Islamic Azad University, Qom, Iran.

ABSTRACT

Background and objectives: The obligate intracellular bacterium Chlamydia trachomatis causes sexually transmissible diseases in human. Timely and sensitive detection of this pathogen is very important. There are many cross-reactions in bacteriological and serological methods in detection of this type of pathogens. The aim of this study was to achieve a more specific antigen for serological tests.

Materials and methods: Blood samples were taken from 192 women with suspected chlamydial infection and sera were isolated. ELISA plate wells were coated with recombinant C. trachomatis OMP2 as antigen. Cut-off system was determined with 40 negative sera. The final results of this research were compared with Euroimmun commercial kit.

Results: The ELISA system cut-off was calculated at 0.27 using negative sera samples. ODs of positive samples were higher than 0.27 and negative samples were lower than it. We obtained 30 samples (15.62%) as positive and 162 cases (84.37%) as negative. Sensitivity and specificity of the recombinant antigen were 90% and 86%, respectively. This antigen showed no cross-reactivity with sera of patients infected with Hydatid cyst, HCV, Epstein barr virus, HBV, Helicobacter pylori, Toxoplasma gondii, Cytomegalovirus, Mycoplasma, Measles and Varicella zoster virus.

Conclusion: The sensitivity and specificity of rOMP2 in ELISA for detection of C. trachomatis were 90% and 86%, respectively. Though the sensitivity was higher than results of Euroimmun commercial kit, its specificity was calculated lower than reference kit.

No MeSH data available.


Related in: MedlinePlus