Limits...
Heterogeneity of Iranian clinical isolates of Mycobacterium fortuitum.

Hashemi-Shahraki A, Heidarieh P, Biranvand M, Bostanabad SZ, Sheikhi N, Hashemzadeh M, Karami M, Feizabadi MM - Iran J Microbiol (2014)

Bottom Line: This study showed that the ITS region possesses high discriminatory power between the clinical isolates up to the clonal level.The results also suggest the possibility of the existence of predominant clone of M. fortuitum in affected patients in Iran.The data also point to the conclusion that a large variety of M. fortuitum clone can produce disease although certain clones seem to be predominant.

View Article: PubMed Central - PubMed

Affiliation: Infectious and Tropical Diseases Research Centre, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

ABSTRACT

Background and objectives: The increase of infections caused by nontuberculous mycobacteria (NTM) is receiving increasing attention worldwide. Mycobacterium fortuitum is encountered with increasing frequency in clinical laboratories of Iran.

Materials and methods: Sequence variation of 48 M. fortuitum clinical isolates, were investigated by sequence analysis of the 16S-23S Internal Transcribed Spacer.

Results: Twelve different sequence types (sequevar) were identified by sequence analysis of ITS region. Seven previously described sequevar including MfoA, MfoB, MfoC, MfoD, MfoE, MfoF and MfoG identified. Five novel sequevar namely MfoH, MfoI, MfoJ, MfoK and MfoL that were distinctly different from the previously described sequevar were detected among different clinical strains of M. fortuitum, from Iran.

Conclusion: This study showed that the ITS region possesses high discriminatory power between the clinical isolates up to the clonal level. The results also suggest the possibility of the existence of predominant clone of M. fortuitum in affected patients in Iran. The data also point to the conclusion that a large variety of M. fortuitum clone can produce disease although certain clones seem to be predominant.

No MeSH data available.


Related in: MedlinePlus

Distance matrix tree showing the divergence of ITS sequences of the Iranaian clinical isolates of M. fortuitum. All alignment positions which are occupied by residues were used for the calculation of binary distance values. The topology of the tree was evaluated and corrected according to the results of maximum-parsimony and maximum-likelihood analyses. The bar represents 0.1 estimated sequence divergence.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4419039&req=5

Figure 1: Distance matrix tree showing the divergence of ITS sequences of the Iranaian clinical isolates of M. fortuitum. All alignment positions which are occupied by residues were used for the calculation of binary distance values. The topology of the tree was evaluated and corrected according to the results of maximum-parsimony and maximum-likelihood analyses. The bar represents 0.1 estimated sequence divergence.

Mentions: Pairwise comparisons between the previously reported sequevar and clinical isolates displayed higher sequence variation between M. fortuitum strains. A dendrogram based on maximum parsimony analysis reflecting the ITS sequence-based clustering of all test strains of M. fortuitum is shown in Fig. 1. Within the consensus tree, twelve clusters with distinct branches among the M. fortuitum reference strains could be defined.


Heterogeneity of Iranian clinical isolates of Mycobacterium fortuitum.

Hashemi-Shahraki A, Heidarieh P, Biranvand M, Bostanabad SZ, Sheikhi N, Hashemzadeh M, Karami M, Feizabadi MM - Iran J Microbiol (2014)

Distance matrix tree showing the divergence of ITS sequences of the Iranaian clinical isolates of M. fortuitum. All alignment positions which are occupied by residues were used for the calculation of binary distance values. The topology of the tree was evaluated and corrected according to the results of maximum-parsimony and maximum-likelihood analyses. The bar represents 0.1 estimated sequence divergence.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4419039&req=5

Figure 1: Distance matrix tree showing the divergence of ITS sequences of the Iranaian clinical isolates of M. fortuitum. All alignment positions which are occupied by residues were used for the calculation of binary distance values. The topology of the tree was evaluated and corrected according to the results of maximum-parsimony and maximum-likelihood analyses. The bar represents 0.1 estimated sequence divergence.
Mentions: Pairwise comparisons between the previously reported sequevar and clinical isolates displayed higher sequence variation between M. fortuitum strains. A dendrogram based on maximum parsimony analysis reflecting the ITS sequence-based clustering of all test strains of M. fortuitum is shown in Fig. 1. Within the consensus tree, twelve clusters with distinct branches among the M. fortuitum reference strains could be defined.

Bottom Line: This study showed that the ITS region possesses high discriminatory power between the clinical isolates up to the clonal level.The results also suggest the possibility of the existence of predominant clone of M. fortuitum in affected patients in Iran.The data also point to the conclusion that a large variety of M. fortuitum clone can produce disease although certain clones seem to be predominant.

View Article: PubMed Central - PubMed

Affiliation: Infectious and Tropical Diseases Research Centre, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

ABSTRACT

Background and objectives: The increase of infections caused by nontuberculous mycobacteria (NTM) is receiving increasing attention worldwide. Mycobacterium fortuitum is encountered with increasing frequency in clinical laboratories of Iran.

Materials and methods: Sequence variation of 48 M. fortuitum clinical isolates, were investigated by sequence analysis of the 16S-23S Internal Transcribed Spacer.

Results: Twelve different sequence types (sequevar) were identified by sequence analysis of ITS region. Seven previously described sequevar including MfoA, MfoB, MfoC, MfoD, MfoE, MfoF and MfoG identified. Five novel sequevar namely MfoH, MfoI, MfoJ, MfoK and MfoL that were distinctly different from the previously described sequevar were detected among different clinical strains of M. fortuitum, from Iran.

Conclusion: This study showed that the ITS region possesses high discriminatory power between the clinical isolates up to the clonal level. The results also suggest the possibility of the existence of predominant clone of M. fortuitum in affected patients in Iran. The data also point to the conclusion that a large variety of M. fortuitum clone can produce disease although certain clones seem to be predominant.

No MeSH data available.


Related in: MedlinePlus