Limits...
CD38 is expressed on inflammatory cells of the intestine and promotes intestinal inflammation.

Schneider M, Schumacher V, Lischke T, Lücke K, Meyer-Schwesinger C, Velden J, Koch-Nolte F, Mittrücker HW - PLoS ONE (2015)

Bottom Line: The enzyme CD38 is expressed on a variety of hematopoietic and non-hematopoietic cells and is involved in diverse processes such as generation of calcium-mobilizing metabolites, cell activation, and chemotaxis.Myeloid cells recruited to this tissue upon inflammation also express enhanced levels of CD38.In conclusion, our results identify a function for CD38 in the control of inflammatory processes in the colon.

View Article: PubMed Central - PubMed

Affiliation: Institute of Immunology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.

ABSTRACT
The enzyme CD38 is expressed on a variety of hematopoietic and non-hematopoietic cells and is involved in diverse processes such as generation of calcium-mobilizing metabolites, cell activation, and chemotaxis. Here, we show that under homeostatic conditions CD38 is highly expressed on immune cells of the colon mucosa of C57BL/6 mice. Myeloid cells recruited to this tissue upon inflammation also express enhanced levels of CD38. To determine the role of CD38 in intestinal inflammation, we applied the dextran sulfate sodium (DSS) colitis model. Whereas wild-type mice developed severe colitis, CD38-/- mice had only mild disease following DSS-treatment. Histologic examination of the colon mucosa revealed pronounced inflammatory damage with dense infiltrates containing numerous granulocytes and macrophages in wild-type animals, while these findings were significantly attenuated in CD38-/- mice. Despite attenuated histological findings, the mRNA expression of inflammatory cytokines and chemokines was only marginally lower in the colons of CD38-/- mice as compared to wild-type mice. In conclusion, our results identify a function for CD38 in the control of inflammatory processes in the colon.

No MeSH data available.


Related in: MedlinePlus

Morphological changes in the colons of wild-type and CD38-/- mice after DSS treatment.Wild-type and CD38-/- mice were treated with DSS as in Fig 1. On day 7, mice were killed and colon sections were H&E stained. (A) Representative H&E stained sections of colons from DSS-treated wild-type (a, a’) and CD38-/- mice (b, b’). Original magnification: 200×. (B). Colon sections were evaluated for tissue damage (score 0–4) and for cellular infiltration (score 0–4), and both scores were added up to a histological score. Parameters of scoring are given in the method section. The figure gives results for individual mice and the median of 6 mice per group. * p<0.05.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4418770&req=5

pone.0126007.g004: Morphological changes in the colons of wild-type and CD38-/- mice after DSS treatment.Wild-type and CD38-/- mice were treated with DSS as in Fig 1. On day 7, mice were killed and colon sections were H&E stained. (A) Representative H&E stained sections of colons from DSS-treated wild-type (a, a’) and CD38-/- mice (b, b’). Original magnification: 200×. (B). Colon sections were evaluated for tissue damage (score 0–4) and for cellular infiltration (score 0–4), and both scores were added up to a histological score. Parameters of scoring are given in the method section. The figure gives results for individual mice and the median of 6 mice per group. * p<0.05.

Mentions: Histological sections of the colon from DSS-treated wild-type and CD38-/- mice were analyzed for cellular infiltration and alterations in the morphology of the epithelium, crypts and submucosa (Fig 4A). Scoring of these parameters revealed severe inflammation in wild-type mice (Fig 4B). In contrast, CD38-/- mice presented with significantly reduced colonic inflammation and damage scores. Immunohistology further indicated that infiltration of the colonic mucosa by both granulocytes and macrophages was less pronounced in CD38-/- mice as compared to wild-type mice (Fig 5). Taken together, these results indicate that CD38 mice are substantially less susceptible to DSS-induced colitis.


CD38 is expressed on inflammatory cells of the intestine and promotes intestinal inflammation.

Schneider M, Schumacher V, Lischke T, Lücke K, Meyer-Schwesinger C, Velden J, Koch-Nolte F, Mittrücker HW - PLoS ONE (2015)

Morphological changes in the colons of wild-type and CD38-/- mice after DSS treatment.Wild-type and CD38-/- mice were treated with DSS as in Fig 1. On day 7, mice were killed and colon sections were H&E stained. (A) Representative H&E stained sections of colons from DSS-treated wild-type (a, a’) and CD38-/- mice (b, b’). Original magnification: 200×. (B). Colon sections were evaluated for tissue damage (score 0–4) and for cellular infiltration (score 0–4), and both scores were added up to a histological score. Parameters of scoring are given in the method section. The figure gives results for individual mice and the median of 6 mice per group. * p<0.05.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4418770&req=5

pone.0126007.g004: Morphological changes in the colons of wild-type and CD38-/- mice after DSS treatment.Wild-type and CD38-/- mice were treated with DSS as in Fig 1. On day 7, mice were killed and colon sections were H&E stained. (A) Representative H&E stained sections of colons from DSS-treated wild-type (a, a’) and CD38-/- mice (b, b’). Original magnification: 200×. (B). Colon sections were evaluated for tissue damage (score 0–4) and for cellular infiltration (score 0–4), and both scores were added up to a histological score. Parameters of scoring are given in the method section. The figure gives results for individual mice and the median of 6 mice per group. * p<0.05.
Mentions: Histological sections of the colon from DSS-treated wild-type and CD38-/- mice were analyzed for cellular infiltration and alterations in the morphology of the epithelium, crypts and submucosa (Fig 4A). Scoring of these parameters revealed severe inflammation in wild-type mice (Fig 4B). In contrast, CD38-/- mice presented with significantly reduced colonic inflammation and damage scores. Immunohistology further indicated that infiltration of the colonic mucosa by both granulocytes and macrophages was less pronounced in CD38-/- mice as compared to wild-type mice (Fig 5). Taken together, these results indicate that CD38 mice are substantially less susceptible to DSS-induced colitis.

Bottom Line: The enzyme CD38 is expressed on a variety of hematopoietic and non-hematopoietic cells and is involved in diverse processes such as generation of calcium-mobilizing metabolites, cell activation, and chemotaxis.Myeloid cells recruited to this tissue upon inflammation also express enhanced levels of CD38.In conclusion, our results identify a function for CD38 in the control of inflammatory processes in the colon.

View Article: PubMed Central - PubMed

Affiliation: Institute of Immunology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.

ABSTRACT
The enzyme CD38 is expressed on a variety of hematopoietic and non-hematopoietic cells and is involved in diverse processes such as generation of calcium-mobilizing metabolites, cell activation, and chemotaxis. Here, we show that under homeostatic conditions CD38 is highly expressed on immune cells of the colon mucosa of C57BL/6 mice. Myeloid cells recruited to this tissue upon inflammation also express enhanced levels of CD38. To determine the role of CD38 in intestinal inflammation, we applied the dextran sulfate sodium (DSS) colitis model. Whereas wild-type mice developed severe colitis, CD38-/- mice had only mild disease following DSS-treatment. Histologic examination of the colon mucosa revealed pronounced inflammatory damage with dense infiltrates containing numerous granulocytes and macrophages in wild-type animals, while these findings were significantly attenuated in CD38-/- mice. Despite attenuated histological findings, the mRNA expression of inflammatory cytokines and chemokines was only marginally lower in the colons of CD38-/- mice as compared to wild-type mice. In conclusion, our results identify a function for CD38 in the control of inflammatory processes in the colon.

No MeSH data available.


Related in: MedlinePlus