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Morphological Observations and Fatty Acid Composition of Indoor-Cultivated Cordyceps sinensis at a High-Altitude Laboratory on Sejila Mountain, Tibet.

Guo LX, Xu XM, Liang FR, Yuan JP, Peng J, Wu CF, Wang JH - PLoS ONE (2015)

Bottom Line: Their fatty acid composition shows a significant difference in the levels of polyunsaturated fatty acids (PUFAs).These observations and fatty acid data suggest that environmental factors, particularly temperature, soil pressure and light intensity, strongly affect the growth of C. sinensis.Our new findings may provide important information for improving techniques for the large-scale artificial cultivation of C. sinensis.

View Article: PubMed Central - PubMed

Affiliation: School of Public Health, Guangdong Medical College, Dongguan, Guangdong, People's Republic of China; Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, Sun Yat-Sen University, Guangzhou, Guangdong, People's Republic of China.

ABSTRACT
Cordyceps sinensis, a caterpillar entomopathogenic fungus-host larva complex, is a rare medicinal herb found in the Qinghai-Tibetan Plateau and its surrounding high-altitude areas. The alternation of generations in the life cycle, whatever the fungus or its host insect, requires special growth conditions. However, it is difficult to simulate the growth conditions of C. sinensis, which hinders its artificial cultivation. In this work, the life cycle from the host larva to C. sinensis was observed in an indoor-cultivation laboratory at 4,200 m a.s.l. on Sejila Mountain, Tibet. Comparative examinations between indoor-cultivated and wild C. sinensis demonstrated that the indoor-cultivated C. sinensis preferred to germinate multiple long, slim stromata at diverse positions on dead larvae, including but not limited to their heads. Their fatty acid composition shows a significant difference in the levels of polyunsaturated fatty acids (PUFAs). In indoor-cultivated C. sinensis, PUFAs constituted 24.59% and 49.43%, respectively, of neutral and polar lipids; meanwhile, in wild C. sinensis, PUFAs represented 34.34% and 61.25% of neutral and polar lipids, respectively. These observations and fatty acid data suggest that environmental factors, particularly temperature, soil pressure and light intensity, strongly affect the growth of C. sinensis. Our new findings may provide important information for improving techniques for the large-scale artificial cultivation of C. sinensis.

No MeSH data available.


Related in: MedlinePlus

Total ion currents (TIC) of gas chromatography-mass spectrometry for the neutral (A) and polar (B) lipids from Cordyceps sinensis.C16:0, palmitic acid methyl ester; C18:0, stearic acid methyl ester; C18:1, oleic acid methyl ester; C18:2, linoleic acid; and C19:0, methylnonadecanoate as an internal standard.
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pone.0126095.g003: Total ion currents (TIC) of gas chromatography-mass spectrometry for the neutral (A) and polar (B) lipids from Cordyceps sinensis.C16:0, palmitic acid methyl ester; C18:0, stearic acid methyl ester; C18:1, oleic acid methyl ester; C18:2, linoleic acid; and C19:0, methylnonadecanoate as an internal standard.

Mentions: Seventeen fatty acids were identified in the neutral and polar lipids from indoor C. sinensis using GC-MS (Fig 3), and their relative abundances and total polyunsaturated fatty acids (PUFAs) are presented in Tables 1 and 2, respectively. The major fatty acids among the neutral lipids were oleic acid (C18:1,cis-9, 49.79–59.13%), linoleic acid (C18:2,cis-9,12, 16.73–31.38%), and palmitic acid (C16:0, 12.73–25.74%); the minor fatty acids were palmitoleic acid (C16:1,cis-9, 0.43–2.31%), stearic acid (C18:0, 0.81–2.14%) and -linolenic acid (C18:3, 0.64–2.19%). The major fatty acids among the polar lipids were linoleic acid (C18:2,cis-9,12, 41.48–54.95%), oleic acid (C18:1,cis-9, 22.01–32.29%), palmitic acid (C16:0, 12.41–20.75%) and stearic acid (C18:0, 3.61–7.00%); the minor fatty acids were palmitoleic acid (C16:1,cis-9, 0.19–1.77%) and -linolenic acid (C18:3, 0.60 ~ 2.31%). In addition, myristic acid (C14:0), myristoleic acid (C14:1,cis-9), heptadecanoic acid (C17:0), heptadecenoic acid (C17:1,cis-9), eicosanoic acid (C20:1,cis-11), eicosadienoic acid (C20:2,cis-11,14), tetracosanoic acid (C24:0), pentadecanoic acid (C15:0), arachidic acid (C20:0), docosanoic acid (C22:0) and docosatetraenoic acid (C22:4,cis-5,8,11,14) were detected in minor amounts in both the neutral and polar lipids from indoor C. sinensis.


Morphological Observations and Fatty Acid Composition of Indoor-Cultivated Cordyceps sinensis at a High-Altitude Laboratory on Sejila Mountain, Tibet.

Guo LX, Xu XM, Liang FR, Yuan JP, Peng J, Wu CF, Wang JH - PLoS ONE (2015)

Total ion currents (TIC) of gas chromatography-mass spectrometry for the neutral (A) and polar (B) lipids from Cordyceps sinensis.C16:0, palmitic acid methyl ester; C18:0, stearic acid methyl ester; C18:1, oleic acid methyl ester; C18:2, linoleic acid; and C19:0, methylnonadecanoate as an internal standard.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4418754&req=5

pone.0126095.g003: Total ion currents (TIC) of gas chromatography-mass spectrometry for the neutral (A) and polar (B) lipids from Cordyceps sinensis.C16:0, palmitic acid methyl ester; C18:0, stearic acid methyl ester; C18:1, oleic acid methyl ester; C18:2, linoleic acid; and C19:0, methylnonadecanoate as an internal standard.
Mentions: Seventeen fatty acids were identified in the neutral and polar lipids from indoor C. sinensis using GC-MS (Fig 3), and their relative abundances and total polyunsaturated fatty acids (PUFAs) are presented in Tables 1 and 2, respectively. The major fatty acids among the neutral lipids were oleic acid (C18:1,cis-9, 49.79–59.13%), linoleic acid (C18:2,cis-9,12, 16.73–31.38%), and palmitic acid (C16:0, 12.73–25.74%); the minor fatty acids were palmitoleic acid (C16:1,cis-9, 0.43–2.31%), stearic acid (C18:0, 0.81–2.14%) and -linolenic acid (C18:3, 0.64–2.19%). The major fatty acids among the polar lipids were linoleic acid (C18:2,cis-9,12, 41.48–54.95%), oleic acid (C18:1,cis-9, 22.01–32.29%), palmitic acid (C16:0, 12.41–20.75%) and stearic acid (C18:0, 3.61–7.00%); the minor fatty acids were palmitoleic acid (C16:1,cis-9, 0.19–1.77%) and -linolenic acid (C18:3, 0.60 ~ 2.31%). In addition, myristic acid (C14:0), myristoleic acid (C14:1,cis-9), heptadecanoic acid (C17:0), heptadecenoic acid (C17:1,cis-9), eicosanoic acid (C20:1,cis-11), eicosadienoic acid (C20:2,cis-11,14), tetracosanoic acid (C24:0), pentadecanoic acid (C15:0), arachidic acid (C20:0), docosanoic acid (C22:0) and docosatetraenoic acid (C22:4,cis-5,8,11,14) were detected in minor amounts in both the neutral and polar lipids from indoor C. sinensis.

Bottom Line: Their fatty acid composition shows a significant difference in the levels of polyunsaturated fatty acids (PUFAs).These observations and fatty acid data suggest that environmental factors, particularly temperature, soil pressure and light intensity, strongly affect the growth of C. sinensis.Our new findings may provide important information for improving techniques for the large-scale artificial cultivation of C. sinensis.

View Article: PubMed Central - PubMed

Affiliation: School of Public Health, Guangdong Medical College, Dongguan, Guangdong, People's Republic of China; Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, Sun Yat-Sen University, Guangzhou, Guangdong, People's Republic of China.

ABSTRACT
Cordyceps sinensis, a caterpillar entomopathogenic fungus-host larva complex, is a rare medicinal herb found in the Qinghai-Tibetan Plateau and its surrounding high-altitude areas. The alternation of generations in the life cycle, whatever the fungus or its host insect, requires special growth conditions. However, it is difficult to simulate the growth conditions of C. sinensis, which hinders its artificial cultivation. In this work, the life cycle from the host larva to C. sinensis was observed in an indoor-cultivation laboratory at 4,200 m a.s.l. on Sejila Mountain, Tibet. Comparative examinations between indoor-cultivated and wild C. sinensis demonstrated that the indoor-cultivated C. sinensis preferred to germinate multiple long, slim stromata at diverse positions on dead larvae, including but not limited to their heads. Their fatty acid composition shows a significant difference in the levels of polyunsaturated fatty acids (PUFAs). In indoor-cultivated C. sinensis, PUFAs constituted 24.59% and 49.43%, respectively, of neutral and polar lipids; meanwhile, in wild C. sinensis, PUFAs represented 34.34% and 61.25% of neutral and polar lipids, respectively. These observations and fatty acid data suggest that environmental factors, particularly temperature, soil pressure and light intensity, strongly affect the growth of C. sinensis. Our new findings may provide important information for improving techniques for the large-scale artificial cultivation of C. sinensis.

No MeSH data available.


Related in: MedlinePlus