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Development and characterization of a recombinant, hypoallergenic, peptide-based vaccine for grass pollen allergy.

Focke-Tejkl M, Weber M, Niespodziana K, Neubauer A, Huber H, Henning R, Stegfellner G, Maderegger B, Hauer M, Stolz F, Niederberger V, Marth K, Eckl-Dorna J, Weiss R, Thalhamer J, Blatt K, Valent P, Valenta R - J. Allergy Clin. Immunol. (2014)

Bottom Line: BM321, BM322, BM325, and BM326 showed almost completely abolished allergenic activity and induced significantly reduced T-cell proliferation and release of proinflammatory cytokines in patients' PBMCs compared with grass pollen allergens.On immunization, they induced allergen-specific IgG antibodies, which inhibited patients' IgE binding to all 4 major allergens of grass pollen, as well as allergen-induced basophil activation.A recombinant hypoallergenic grass pollen allergy vaccine (BM32) consisting of 4 recombinant PreS-fused grass pollen allergen peptides was developed for safe immunotherapy of grass pollen allergy.

View Article: PubMed Central - PubMed

Affiliation: Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center of Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.

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IgG reactivity of sera from peptide-immunized rabbits with Phl p 2 (A) and Phl p 6 (B). IgG reactivities (mean ± SD OD levels of triplicates; y-axes) to Phl p 2 (Fig E3, A) or Phl p 6 (Fig E3, B) determined for different dilutions (x-axes) of sera from rabbits immunized with the complete allergens or KLH-coupled allergen-derived peptides are shown (Fig E3, A: Phl p 2 and Phl p 2 peptides P1-P4; Fig E3, B: Phl p 6 and Phl p 6 peptides P1-P4).
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dfig3: IgG reactivity of sera from peptide-immunized rabbits with Phl p 2 (A) and Phl p 6 (B). IgG reactivities (mean ± SD OD levels of triplicates; y-axes) to Phl p 2 (Fig E3, A) or Phl p 6 (Fig E3, B) determined for different dilutions (x-axes) of sera from rabbits immunized with the complete allergens or KLH-coupled allergen-derived peptides are shown (Fig E3, A: Phl p 2 and Phl p 2 peptides P1-P4; Fig E3, B: Phl p 6 and Phl p 6 peptides P1-P4).

Mentions: Next, we investigated which peptides induce allergen-specific IgG responses on immunization. Fig E3 in this article's Online Repository at www.jacionline.org shows reactivities of peptide-specific IgG antibodies with the corresponding allergens. For each of the 2 allergens, one peptide (ie, Phl p 2: P4-2; Phl p 6: P1-6) could be identified that induced levels of IgG antibodies comparable to those induced by the complete allergen (see Fig E3).


Development and characterization of a recombinant, hypoallergenic, peptide-based vaccine for grass pollen allergy.

Focke-Tejkl M, Weber M, Niespodziana K, Neubauer A, Huber H, Henning R, Stegfellner G, Maderegger B, Hauer M, Stolz F, Niederberger V, Marth K, Eckl-Dorna J, Weiss R, Thalhamer J, Blatt K, Valent P, Valenta R - J. Allergy Clin. Immunol. (2014)

IgG reactivity of sera from peptide-immunized rabbits with Phl p 2 (A) and Phl p 6 (B). IgG reactivities (mean ± SD OD levels of triplicates; y-axes) to Phl p 2 (Fig E3, A) or Phl p 6 (Fig E3, B) determined for different dilutions (x-axes) of sera from rabbits immunized with the complete allergens or KLH-coupled allergen-derived peptides are shown (Fig E3, A: Phl p 2 and Phl p 2 peptides P1-P4; Fig E3, B: Phl p 6 and Phl p 6 peptides P1-P4).
© Copyright Policy - CC BY
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4418753&req=5

dfig3: IgG reactivity of sera from peptide-immunized rabbits with Phl p 2 (A) and Phl p 6 (B). IgG reactivities (mean ± SD OD levels of triplicates; y-axes) to Phl p 2 (Fig E3, A) or Phl p 6 (Fig E3, B) determined for different dilutions (x-axes) of sera from rabbits immunized with the complete allergens or KLH-coupled allergen-derived peptides are shown (Fig E3, A: Phl p 2 and Phl p 2 peptides P1-P4; Fig E3, B: Phl p 6 and Phl p 6 peptides P1-P4).
Mentions: Next, we investigated which peptides induce allergen-specific IgG responses on immunization. Fig E3 in this article's Online Repository at www.jacionline.org shows reactivities of peptide-specific IgG antibodies with the corresponding allergens. For each of the 2 allergens, one peptide (ie, Phl p 2: P4-2; Phl p 6: P1-6) could be identified that induced levels of IgG antibodies comparable to those induced by the complete allergen (see Fig E3).

Bottom Line: BM321, BM322, BM325, and BM326 showed almost completely abolished allergenic activity and induced significantly reduced T-cell proliferation and release of proinflammatory cytokines in patients' PBMCs compared with grass pollen allergens.On immunization, they induced allergen-specific IgG antibodies, which inhibited patients' IgE binding to all 4 major allergens of grass pollen, as well as allergen-induced basophil activation.A recombinant hypoallergenic grass pollen allergy vaccine (BM32) consisting of 4 recombinant PreS-fused grass pollen allergen peptides was developed for safe immunotherapy of grass pollen allergy.

View Article: PubMed Central - PubMed

Affiliation: Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center of Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.

Show MeSH
Related in: MedlinePlus