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The Reduction of Peripheral Blood CD4+ T Cell Indicates Persistent Organ Failure in Acute Pancreatitis.

Yang Z, Zhang Y, Dong L, Yang C, Gou S, Yin T, Wu H, Wang C - PLoS ONE (2015)

Bottom Line: Patients with POF showed a significantly higher value of IL-1β and hs-CRP on day 7 compared with the group of TOF (P < 0.05).Proportions of CD4(+) T cells on days 1, 3, 7 and CD4(+)/ CD8(+) ratio on day 1 were statistically lower in the group of POF patients (P < 0.05).A CD4(+) T cell proportion of 30.34% on day 1 predicted POF with an area under the curve (AUC) of 0.798, a sensitivity with 61.54% and specificity with 90.00%, respectively.

View Article: PubMed Central - PubMed

Affiliation: Pancreatic Disease Institute, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei Province, People's Republic of China.

ABSTRACT

Objective: Few data are available on the potential role of inflammatory mediators and T lymphocytes in persistent organ failure (POF) in acute pancreatitis (AP). We conducted a retrospective study to characterize their role in the progression of POF in AP.

Methods: A total of 69 AP patients presented within 24 hours from symptom onset developing organ failure (OF) on admission were included in our study. There were 39 patients suffering from POF and 30 from transient OF (TOF). On the 1st, 3rd and 7th days after admission, blood samples were collected for biochemical concentration monitoring including serum IL-1β, IL-6, TNF-α and high-sensitivity C-reactive protein (hs-CRP). The proportions of peripheral CD4(+) and CD8(+) T lymphocytes were assessed based on flow cytometry simultaneously.

Results: Patients with POF showed a significantly higher value of IL-1β and hs-CRP on day 7 compared with the group of TOF (P < 0.05). Proportions of CD4(+) T cells on days 1, 3, 7 and CD4(+)/ CD8(+) ratio on day 1 were statistically lower in the group of POF patients (P < 0.05). A CD4(+) T cell proportion of 30.34% on day 1 predicted POF with an area under the curve (AUC) of 0.798, a sensitivity with 61.54% and specificity with 90.00%, respectively.

Conclusions: The reduction of peripheral blood CD4(+) T lymphocytes is associated with POF in AP, and may act as a potential predictor.

No MeSH data available.


Related in: MedlinePlus

Sequential changes in the values of IL-1β (A), IL-6 (B), TNF-α (C) and hs-CRP (D) in the POF and TOF groups.
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pone.0125529.g001: Sequential changes in the values of IL-1β (A), IL-6 (B), TNF-α (C) and hs-CRP (D) in the POF and TOF groups.

Mentions: Serum peak value of IL-6 was observed on day 1, and then decreased rapidly on days 3 and 7 in both POF patients (63.08 ± 48.02 pg/ml vs. 41.10 ± 27.34 pg/ml and 25.24 ± 30.28 pg/ml, P < 0.01) and TOF ones (55.14 ± 48.62 pg/ml vs. 31.84 ± 28.49 pg/ml and 19.94 ± 15.90 pg/ml, P < 0.05). Similarly, serum level of hs-CRP peaked on day 1 and significant reductions were noticed on days 3 and 7 in group of POF (225.74 ± 105.63 mg/L vs. 157.44 ± 55.72 mg/L and 124.06 ± 55.99 mg/L, P < 0.01) and in group of TOF (254.37 ± 88.43 mg/L vs. 171.71 ± 77.56 mg/L and 85.99 ± 38.95 mg/L, P < 0.01). Serum IL-1β and TNF-α showed no statistical significance on days 1, 3 and 7 in both two groups. Values of IL-1β and hs-CRP were significantly lower in the TOF group on day 7 compared with the POF group, while values of IL-6 and TNF-α showed no significant difference between the two groups on all three days (Fig 1).


The Reduction of Peripheral Blood CD4+ T Cell Indicates Persistent Organ Failure in Acute Pancreatitis.

Yang Z, Zhang Y, Dong L, Yang C, Gou S, Yin T, Wu H, Wang C - PLoS ONE (2015)

Sequential changes in the values of IL-1β (A), IL-6 (B), TNF-α (C) and hs-CRP (D) in the POF and TOF groups.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4418709&req=5

pone.0125529.g001: Sequential changes in the values of IL-1β (A), IL-6 (B), TNF-α (C) and hs-CRP (D) in the POF and TOF groups.
Mentions: Serum peak value of IL-6 was observed on day 1, and then decreased rapidly on days 3 and 7 in both POF patients (63.08 ± 48.02 pg/ml vs. 41.10 ± 27.34 pg/ml and 25.24 ± 30.28 pg/ml, P < 0.01) and TOF ones (55.14 ± 48.62 pg/ml vs. 31.84 ± 28.49 pg/ml and 19.94 ± 15.90 pg/ml, P < 0.05). Similarly, serum level of hs-CRP peaked on day 1 and significant reductions were noticed on days 3 and 7 in group of POF (225.74 ± 105.63 mg/L vs. 157.44 ± 55.72 mg/L and 124.06 ± 55.99 mg/L, P < 0.01) and in group of TOF (254.37 ± 88.43 mg/L vs. 171.71 ± 77.56 mg/L and 85.99 ± 38.95 mg/L, P < 0.01). Serum IL-1β and TNF-α showed no statistical significance on days 1, 3 and 7 in both two groups. Values of IL-1β and hs-CRP were significantly lower in the TOF group on day 7 compared with the POF group, while values of IL-6 and TNF-α showed no significant difference between the two groups on all three days (Fig 1).

Bottom Line: Patients with POF showed a significantly higher value of IL-1β and hs-CRP on day 7 compared with the group of TOF (P < 0.05).Proportions of CD4(+) T cells on days 1, 3, 7 and CD4(+)/ CD8(+) ratio on day 1 were statistically lower in the group of POF patients (P < 0.05).A CD4(+) T cell proportion of 30.34% on day 1 predicted POF with an area under the curve (AUC) of 0.798, a sensitivity with 61.54% and specificity with 90.00%, respectively.

View Article: PubMed Central - PubMed

Affiliation: Pancreatic Disease Institute, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei Province, People's Republic of China.

ABSTRACT

Objective: Few data are available on the potential role of inflammatory mediators and T lymphocytes in persistent organ failure (POF) in acute pancreatitis (AP). We conducted a retrospective study to characterize their role in the progression of POF in AP.

Methods: A total of 69 AP patients presented within 24 hours from symptom onset developing organ failure (OF) on admission were included in our study. There were 39 patients suffering from POF and 30 from transient OF (TOF). On the 1st, 3rd and 7th days after admission, blood samples were collected for biochemical concentration monitoring including serum IL-1β, IL-6, TNF-α and high-sensitivity C-reactive protein (hs-CRP). The proportions of peripheral CD4(+) and CD8(+) T lymphocytes were assessed based on flow cytometry simultaneously.

Results: Patients with POF showed a significantly higher value of IL-1β and hs-CRP on day 7 compared with the group of TOF (P < 0.05). Proportions of CD4(+) T cells on days 1, 3, 7 and CD4(+)/ CD8(+) ratio on day 1 were statistically lower in the group of POF patients (P < 0.05). A CD4(+) T cell proportion of 30.34% on day 1 predicted POF with an area under the curve (AUC) of 0.798, a sensitivity with 61.54% and specificity with 90.00%, respectively.

Conclusions: The reduction of peripheral blood CD4(+) T lymphocytes is associated with POF in AP, and may act as a potential predictor.

No MeSH data available.


Related in: MedlinePlus