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Antennal Transcriptome Analysis of Odorant Reception Genes in the Red Turpentine Beetle (RTB), Dendroctonus valens.

Gu XC, Zhang YN, Kang K, Dong SL, Zhang LW - PLoS ONE (2015)

Bottom Line: Predicted protein sequences were compared with counterparts in Tribolium castaneum, Megacyllene caryae, Ips typographus, Dendroctonus ponderosae, and Agrilus planipennis.The genes reported here provide a significant addition to the pool of identified olfactory genes in Coleoptera, which might represent novel targets for insect management.The results from our study also will assist with evolutionary analyses of coleopteran olfaction.

View Article: PubMed Central - PubMed

Affiliation: Anhui Provincial Key Laboratory of Microbial Control, School of Forestry & Landscape Architecture, Anhui Agricultural University, Hefei, China.

ABSTRACT

Background: The red turpentine beetle (RTB), Dendroctonus valens LeConte (Coleoptera: Curculionidae, Scolytinae), is a destructive invasive pest of conifers which has become the second most important forest pest nationwide in China. Dendroctonus valens is known to use host odors and aggregation pheromones, as well as non-host volatiles, in host location and mass-attack modulation, and thus antennal olfaction is of the utmost importance for the beetles' survival and fitness. However, information on the genes underlying olfaction has been lacking in D. valens. Here, we report the antennal transcriptome of D. valens from next-generation sequencing, with the goal of identifying the olfaction gene repertoire that is involved in D. valens odor-processing.

Results: We obtained 51 million reads that were assembled into 61,889 genes, including 39,831 contigs and 22,058 unigenes. In total, we identified 68 novel putative odorant reception genes, including 21 transcripts encoding for putative odorant binding proteins (OBP), six chemosensory proteins (CSP), four sensory neuron membrane proteins (SNMP), 22 odorant receptors (OR), four gustatory receptors (GR), three ionotropic receptors (IR), and eight ionotropic glutamate receptors. We also identified 155 odorant/xenobiotic degradation enzymes from the antennal transcriptome, putatively identified to be involved in olfaction processes including cytochrome P450s, glutathione-S-transferases, and aldehyde dehydrogenase. Predicted protein sequences were compared with counterparts in Tribolium castaneum, Megacyllene caryae, Ips typographus, Dendroctonus ponderosae, and Agrilus planipennis.

Conclusion: The antennal transcriptome described here represents the first study of the repertoire of odor processing genes in D. valens. The genes reported here provide a significant addition to the pool of identified olfactory genes in Coleoptera, which might represent novel targets for insect management. The results from our study also will assist with evolutionary analyses of coleopteran olfaction.

No MeSH data available.


Related in: MedlinePlus

Phylogenetic tree of putative OBPs from Dendroctonus valens (Dval), Ips typographus (Ityp), Dendroctonus ponderosae (Dpon), Tribolium castaneum (Tcas) and Agrilus planipennis (Ap).The D. valens translated unigenes are shown in blue. Amino acid sequences are given in S1 Fig. The tree was constructed with MEGA5.0, using the neighbor-joining method. Values indicated at the nodes are bootstrap values based on 1000 replicates, and the bootstrap values below 50% are not shown.
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pone.0125159.g005: Phylogenetic tree of putative OBPs from Dendroctonus valens (Dval), Ips typographus (Ityp), Dendroctonus ponderosae (Dpon), Tribolium castaneum (Tcas) and Agrilus planipennis (Ap).The D. valens translated unigenes are shown in blue. Amino acid sequences are given in S1 Fig. The tree was constructed with MEGA5.0, using the neighbor-joining method. Values indicated at the nodes are bootstrap values based on 1000 replicates, and the bootstrap values below 50% are not shown.

Mentions: We identified 21 transcripts encoding putative OBPs in the D. valens antennal transcriptome. The number of putative OBP-coding genes in D. valens is less than that of D. ponderosae (31), but more than I. typographus (15). Sequence analysis identified 18 unigenes with a full length ORF (Open Reading Frame) with predicted signal peptide sequences (Table 2). Twenty of the 21 putative OBPs had high similarity to known coleopteran OBPs, (Table 2). DvalOBP3, 4, 7, 10, 14, 16, 18, and 21 had very high similarity with DponOBP6, 5, 4, 30, 8, 12, 19, and 18, respectively, with at least 95% identity (Table 2). A phylogenetic tree based on the neighbor-joining method is shown in Fig 5. Among the 21 putative OBPs, 19 sequences were clustered with at least one coleopteran orthologous gene (Fig 5). Not surprisingly, the identified DvalOBPs sequences were mostly clustered together with DponOBPs, and fourteen OBP orthologous pairs shared high amino acid identity (>85%) between the two species (Fig 5 and Table 2). The amino acid sequences of all OBPs are listed in S1 Fig.


Antennal Transcriptome Analysis of Odorant Reception Genes in the Red Turpentine Beetle (RTB), Dendroctonus valens.

Gu XC, Zhang YN, Kang K, Dong SL, Zhang LW - PLoS ONE (2015)

Phylogenetic tree of putative OBPs from Dendroctonus valens (Dval), Ips typographus (Ityp), Dendroctonus ponderosae (Dpon), Tribolium castaneum (Tcas) and Agrilus planipennis (Ap).The D. valens translated unigenes are shown in blue. Amino acid sequences are given in S1 Fig. The tree was constructed with MEGA5.0, using the neighbor-joining method. Values indicated at the nodes are bootstrap values based on 1000 replicates, and the bootstrap values below 50% are not shown.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4418697&req=5

pone.0125159.g005: Phylogenetic tree of putative OBPs from Dendroctonus valens (Dval), Ips typographus (Ityp), Dendroctonus ponderosae (Dpon), Tribolium castaneum (Tcas) and Agrilus planipennis (Ap).The D. valens translated unigenes are shown in blue. Amino acid sequences are given in S1 Fig. The tree was constructed with MEGA5.0, using the neighbor-joining method. Values indicated at the nodes are bootstrap values based on 1000 replicates, and the bootstrap values below 50% are not shown.
Mentions: We identified 21 transcripts encoding putative OBPs in the D. valens antennal transcriptome. The number of putative OBP-coding genes in D. valens is less than that of D. ponderosae (31), but more than I. typographus (15). Sequence analysis identified 18 unigenes with a full length ORF (Open Reading Frame) with predicted signal peptide sequences (Table 2). Twenty of the 21 putative OBPs had high similarity to known coleopteran OBPs, (Table 2). DvalOBP3, 4, 7, 10, 14, 16, 18, and 21 had very high similarity with DponOBP6, 5, 4, 30, 8, 12, 19, and 18, respectively, with at least 95% identity (Table 2). A phylogenetic tree based on the neighbor-joining method is shown in Fig 5. Among the 21 putative OBPs, 19 sequences were clustered with at least one coleopteran orthologous gene (Fig 5). Not surprisingly, the identified DvalOBPs sequences were mostly clustered together with DponOBPs, and fourteen OBP orthologous pairs shared high amino acid identity (>85%) between the two species (Fig 5 and Table 2). The amino acid sequences of all OBPs are listed in S1 Fig.

Bottom Line: Predicted protein sequences were compared with counterparts in Tribolium castaneum, Megacyllene caryae, Ips typographus, Dendroctonus ponderosae, and Agrilus planipennis.The genes reported here provide a significant addition to the pool of identified olfactory genes in Coleoptera, which might represent novel targets for insect management.The results from our study also will assist with evolutionary analyses of coleopteran olfaction.

View Article: PubMed Central - PubMed

Affiliation: Anhui Provincial Key Laboratory of Microbial Control, School of Forestry & Landscape Architecture, Anhui Agricultural University, Hefei, China.

ABSTRACT

Background: The red turpentine beetle (RTB), Dendroctonus valens LeConte (Coleoptera: Curculionidae, Scolytinae), is a destructive invasive pest of conifers which has become the second most important forest pest nationwide in China. Dendroctonus valens is known to use host odors and aggregation pheromones, as well as non-host volatiles, in host location and mass-attack modulation, and thus antennal olfaction is of the utmost importance for the beetles' survival and fitness. However, information on the genes underlying olfaction has been lacking in D. valens. Here, we report the antennal transcriptome of D. valens from next-generation sequencing, with the goal of identifying the olfaction gene repertoire that is involved in D. valens odor-processing.

Results: We obtained 51 million reads that were assembled into 61,889 genes, including 39,831 contigs and 22,058 unigenes. In total, we identified 68 novel putative odorant reception genes, including 21 transcripts encoding for putative odorant binding proteins (OBP), six chemosensory proteins (CSP), four sensory neuron membrane proteins (SNMP), 22 odorant receptors (OR), four gustatory receptors (GR), three ionotropic receptors (IR), and eight ionotropic glutamate receptors. We also identified 155 odorant/xenobiotic degradation enzymes from the antennal transcriptome, putatively identified to be involved in olfaction processes including cytochrome P450s, glutathione-S-transferases, and aldehyde dehydrogenase. Predicted protein sequences were compared with counterparts in Tribolium castaneum, Megacyllene caryae, Ips typographus, Dendroctonus ponderosae, and Agrilus planipennis.

Conclusion: The antennal transcriptome described here represents the first study of the repertoire of odor processing genes in D. valens. The genes reported here provide a significant addition to the pool of identified olfactory genes in Coleoptera, which might represent novel targets for insect management. The results from our study also will assist with evolutionary analyses of coleopteran olfaction.

No MeSH data available.


Related in: MedlinePlus