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Molecular Cloning and Characterisation of Farnesyl Pyrophosphate Synthase from Tripterygium wilfordii.

Zhao YJ, Chen X, Zhang M, Su P, Liu YJ, Tong YR, Wang XJ, Huang LQ, Gao W - PLoS ONE (2015)

Bottom Line: TwFPSs maintained their capability to synthesise FPP in vitro when purified as recombinant proteins from E. coli.Consistent with the endogenous role of FPS in FPP biosynthesis, TwFPSs were highly expressed in T. wilfordii roots, and were up-regulated upon methyl jasmonate (MeJA) treatment.The global gene expression profiles suggested that the TwFPSs might play an important regulatory role interpenoid biosynthesis in T. wilfordii, laying the groundwork for the future study of the synthetic biology of natural terpene products.

View Article: PubMed Central - PubMed

Affiliation: Capital Medical University School of Traditional Chinese Medicine, Capital Medical University, Beijing, People's Republic of China.

ABSTRACT
Farnesylpyrophosphate synthase (FPS) catalyzes the biosynthesis of farnesyl pyrophosphate (FPP), which is an important precursor of sesquiterpenoids such as artemisinin and wilfordine. In the present study, we report the molecular cloning and characterization of two full-length cDNAs encoding FPSs from Tripterygium wilfordii (TwFPSs). TwFPSs maintained their capability to synthesise FPP in vitro when purified as recombinant proteins from E. coli. Consistent with the endogenous role of FPS in FPP biosynthesis, TwFPSs were highly expressed in T. wilfordii roots, and were up-regulated upon methyl jasmonate (MeJA) treatment. The global gene expression profiles suggested that the TwFPSs might play an important regulatory role interpenoid biosynthesis in T. wilfordii, laying the groundwork for the future study of the synthetic biology of natural terpene products.

No MeSH data available.


The main medicinal active substances of Tripterygium wilfordii.
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pone.0125415.g001: The main medicinal active substances of Tripterygium wilfordii.

Mentions: Tripterygium wilfordii is a traditional Chinese medicinal plant used to treat inflammatory diseases because of its analgesic and anti-microbial properties [1]. This plant has been widely used in the treatment of immune and tumour diseases [2–4]. Terpenoids are the primary active substances (Fig 1) of T.wilfordii and include sesquiterpene, diterpenoids andtriterpenoids. Triptolide [5], which has been recognised as one of the primary active constituents of T. wilfordii, is a class of 3 epoxygroups and an α,β-unsaturated five-membered lactone ringand is also a unique abietane diterpene. T. wilfordii has attracted much attention due to its architecture and significant activities. Tripterine [6], which was the first monomer isolated from T. wilfordii has important biological activity.


Molecular Cloning and Characterisation of Farnesyl Pyrophosphate Synthase from Tripterygium wilfordii.

Zhao YJ, Chen X, Zhang M, Su P, Liu YJ, Tong YR, Wang XJ, Huang LQ, Gao W - PLoS ONE (2015)

The main medicinal active substances of Tripterygium wilfordii.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4418688&req=5

pone.0125415.g001: The main medicinal active substances of Tripterygium wilfordii.
Mentions: Tripterygium wilfordii is a traditional Chinese medicinal plant used to treat inflammatory diseases because of its analgesic and anti-microbial properties [1]. This plant has been widely used in the treatment of immune and tumour diseases [2–4]. Terpenoids are the primary active substances (Fig 1) of T.wilfordii and include sesquiterpene, diterpenoids andtriterpenoids. Triptolide [5], which has been recognised as one of the primary active constituents of T. wilfordii, is a class of 3 epoxygroups and an α,β-unsaturated five-membered lactone ringand is also a unique abietane diterpene. T. wilfordii has attracted much attention due to its architecture and significant activities. Tripterine [6], which was the first monomer isolated from T. wilfordii has important biological activity.

Bottom Line: TwFPSs maintained their capability to synthesise FPP in vitro when purified as recombinant proteins from E. coli.Consistent with the endogenous role of FPS in FPP biosynthesis, TwFPSs were highly expressed in T. wilfordii roots, and were up-regulated upon methyl jasmonate (MeJA) treatment.The global gene expression profiles suggested that the TwFPSs might play an important regulatory role interpenoid biosynthesis in T. wilfordii, laying the groundwork for the future study of the synthetic biology of natural terpene products.

View Article: PubMed Central - PubMed

Affiliation: Capital Medical University School of Traditional Chinese Medicine, Capital Medical University, Beijing, People's Republic of China.

ABSTRACT
Farnesylpyrophosphate synthase (FPS) catalyzes the biosynthesis of farnesyl pyrophosphate (FPP), which is an important precursor of sesquiterpenoids such as artemisinin and wilfordine. In the present study, we report the molecular cloning and characterization of two full-length cDNAs encoding FPSs from Tripterygium wilfordii (TwFPSs). TwFPSs maintained their capability to synthesise FPP in vitro when purified as recombinant proteins from E. coli. Consistent with the endogenous role of FPS in FPP biosynthesis, TwFPSs were highly expressed in T. wilfordii roots, and were up-regulated upon methyl jasmonate (MeJA) treatment. The global gene expression profiles suggested that the TwFPSs might play an important regulatory role interpenoid biosynthesis in T. wilfordii, laying the groundwork for the future study of the synthetic biology of natural terpene products.

No MeSH data available.