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Performance Assessment of the BluePoint MycoID Plus Kit for Identification of Mycobacterium tuberculosis, Including Rifampin- and Isoniazid-resistant Isolates, and Nontuberculous Mycobacteria.

Chien JY, Chang TC, Chiu WY, Yu CJ, Hsueh PR - PLoS ONE (2015)

Bottom Line: In identifying specific NTM species, the kit correctly identified 99.3% of M. abscessus (147/148) complex, 100% of M. fortuitum (32/32), M. gordonae (38/38), M. avium (39/39), M. intracellulare (90/90), M. kansasii (36/36), and M. avium complex species other than M. avium and M. intracellulare (94/94).In conclusions, the diagnostic value of the BluePoint MycoID plus kit was superior to culture method for recoveries and identification of NTM to species level.In addition, the diagnostic accuracy of BluePoint MycoID plus kit in MTB identification was similar to conventional culture method with high accuracy rate of rifampicin-resistant M. tuberculosis identification.

View Article: PubMed Central - PubMed

Affiliation: Graduate Institute of Clinical Medicine, National Taiwan University College of Medicine, Taipei, Taiwan; Department of Internal Medicine, National Taiwan University Hospital, National Taiwan University College of Medicine, Taipei, Taiwan; Chest Hospital, Ministry of Health and Welfare, Tainan, Taiwan.

ABSTRACT
The performance of the BluePoint MycoID plus kit (Bio Concept Corporation, Taichung, Taiwan), which was designed to simultaneously detect Mycobacterium tuberculosis (MTB), rifampin- and isoniazid-resistant MTB, and nontuberculous mycobacteria (NTM) was first evaluated with 950 consecutive positive cultures in Mycobacterium Growth Indicator Tube (MGIT) system (BACTEC, MGIT 960 system, Becton-Dickinson, Sparks) from clinical respiratory specimens. The discrepant results between kit and culture-based identification were finally assessed by 16S rRNA gene sequencing and clinical diagnosis. The accuracy rate of this kit for identification of all Mycobacterium species was 96.3% (905/940). For MTB identification, the sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of the kit were 99.7%, 99.3%, 99.0% and 99.8%, respectively. For rifampicin-resistant MTB identification, the sensitivity, specificity, PPV, and NPV of the kit were 100.0%, 99.4%, 91.3%, and 100.0%, respectively, while the corresponding values of isoniazid-resistant MTB identification were 82.6%, 99.4%, 95.0%, and 97.6%, respectively. In identifying specific NTM species, the kit correctly identified 99.3% of M. abscessus (147/148) complex, 100% of M. fortuitum (32/32), M. gordonae (38/38), M. avium (39/39), M. intracellulare (90/90), M. kansasii (36/36), and M. avium complex species other than M. avium and M. intracellulare (94/94). In conclusions, the diagnostic value of the BluePoint MycoID plus kit was superior to culture method for recoveries and identification of NTM to species level. In addition, the diagnostic accuracy of BluePoint MycoID plus kit in MTB identification was similar to conventional culture method with high accuracy rate of rifampicin-resistant M. tuberculosis identification.

No MeSH data available.


Related in: MedlinePlus

The performances of selected mycobacterial species for identification and resistance-associated mutations for isoniazid (inhA and katG) and rifampin (rpoB) of M. tuberculosis isolates by the BluePoint MycoID plus kit among the positive cultures in Mycobacterium Growth Indicator Tubes.
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pone.0125016.g002: The performances of selected mycobacterial species for identification and resistance-associated mutations for isoniazid (inhA and katG) and rifampin (rpoB) of M. tuberculosis isolates by the BluePoint MycoID plus kit among the positive cultures in Mycobacterium Growth Indicator Tubes.

Mentions: DNA was extracted from a positive MGIT culture by the boiling method and the test was done according to the instructions supplied by the manufacturer as previously described [12]]. The layout and designations of oligonucleotide probes and targets for the probes are illustrated in Figs 1 and 2. The test procedure consisted of amplification of the ITS and gyrB regions by a multiplex PCR, hybridization of the digoxigenin-labeled amplicons with the array, and reaction with enzyme-conjugated anti-digoxigenin antibodies. The hybridized spot was read by a simple reader supplied by the kit manufacturer. A strain was identified to the species level when a species-specific probe and the positive control probe were simultaneously hybridized. If only the positive control probe was hybridized, the microorganism was identified to the genus level (Mycobacterium species).


Performance Assessment of the BluePoint MycoID Plus Kit for Identification of Mycobacterium tuberculosis, Including Rifampin- and Isoniazid-resistant Isolates, and Nontuberculous Mycobacteria.

Chien JY, Chang TC, Chiu WY, Yu CJ, Hsueh PR - PLoS ONE (2015)

The performances of selected mycobacterial species for identification and resistance-associated mutations for isoniazid (inhA and katG) and rifampin (rpoB) of M. tuberculosis isolates by the BluePoint MycoID plus kit among the positive cultures in Mycobacterium Growth Indicator Tubes.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4418609&req=5

pone.0125016.g002: The performances of selected mycobacterial species for identification and resistance-associated mutations for isoniazid (inhA and katG) and rifampin (rpoB) of M. tuberculosis isolates by the BluePoint MycoID plus kit among the positive cultures in Mycobacterium Growth Indicator Tubes.
Mentions: DNA was extracted from a positive MGIT culture by the boiling method and the test was done according to the instructions supplied by the manufacturer as previously described [12]]. The layout and designations of oligonucleotide probes and targets for the probes are illustrated in Figs 1 and 2. The test procedure consisted of amplification of the ITS and gyrB regions by a multiplex PCR, hybridization of the digoxigenin-labeled amplicons with the array, and reaction with enzyme-conjugated anti-digoxigenin antibodies. The hybridized spot was read by a simple reader supplied by the kit manufacturer. A strain was identified to the species level when a species-specific probe and the positive control probe were simultaneously hybridized. If only the positive control probe was hybridized, the microorganism was identified to the genus level (Mycobacterium species).

Bottom Line: In identifying specific NTM species, the kit correctly identified 99.3% of M. abscessus (147/148) complex, 100% of M. fortuitum (32/32), M. gordonae (38/38), M. avium (39/39), M. intracellulare (90/90), M. kansasii (36/36), and M. avium complex species other than M. avium and M. intracellulare (94/94).In conclusions, the diagnostic value of the BluePoint MycoID plus kit was superior to culture method for recoveries and identification of NTM to species level.In addition, the diagnostic accuracy of BluePoint MycoID plus kit in MTB identification was similar to conventional culture method with high accuracy rate of rifampicin-resistant M. tuberculosis identification.

View Article: PubMed Central - PubMed

Affiliation: Graduate Institute of Clinical Medicine, National Taiwan University College of Medicine, Taipei, Taiwan; Department of Internal Medicine, National Taiwan University Hospital, National Taiwan University College of Medicine, Taipei, Taiwan; Chest Hospital, Ministry of Health and Welfare, Tainan, Taiwan.

ABSTRACT
The performance of the BluePoint MycoID plus kit (Bio Concept Corporation, Taichung, Taiwan), which was designed to simultaneously detect Mycobacterium tuberculosis (MTB), rifampin- and isoniazid-resistant MTB, and nontuberculous mycobacteria (NTM) was first evaluated with 950 consecutive positive cultures in Mycobacterium Growth Indicator Tube (MGIT) system (BACTEC, MGIT 960 system, Becton-Dickinson, Sparks) from clinical respiratory specimens. The discrepant results between kit and culture-based identification were finally assessed by 16S rRNA gene sequencing and clinical diagnosis. The accuracy rate of this kit for identification of all Mycobacterium species was 96.3% (905/940). For MTB identification, the sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of the kit were 99.7%, 99.3%, 99.0% and 99.8%, respectively. For rifampicin-resistant MTB identification, the sensitivity, specificity, PPV, and NPV of the kit were 100.0%, 99.4%, 91.3%, and 100.0%, respectively, while the corresponding values of isoniazid-resistant MTB identification were 82.6%, 99.4%, 95.0%, and 97.6%, respectively. In identifying specific NTM species, the kit correctly identified 99.3% of M. abscessus (147/148) complex, 100% of M. fortuitum (32/32), M. gordonae (38/38), M. avium (39/39), M. intracellulare (90/90), M. kansasii (36/36), and M. avium complex species other than M. avium and M. intracellulare (94/94). In conclusions, the diagnostic value of the BluePoint MycoID plus kit was superior to culture method for recoveries and identification of NTM to species level. In addition, the diagnostic accuracy of BluePoint MycoID plus kit in MTB identification was similar to conventional culture method with high accuracy rate of rifampicin-resistant M. tuberculosis identification.

No MeSH data available.


Related in: MedlinePlus