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Oropharyngeal Colonization With Neisseria lactamica, Other Nonpathogenic Neisseria Species and Moraxella catarrhalis Among Young Healthy Children in Ahvaz, Iran.

Sheikhi R, Amin M, Rostami S, Shoja S, Ebrahimi N - Jundishapur J Microbiol (2015)

Bottom Line: The PCR and sequencing were used to confirm the accuracy of laboratory diagnosis to identify N. lactamica and M. catarrhalis.The young healthy children screening by colonization of N. lactamica and other nonpathogenic Neisseria spp. in oropharynx was the first report in Ahvaz, Iran.The study results demonstrated the high frequency of colonization of M. catarrhalis in the studied young healthy children other than Neisseria spp.

View Article: PubMed Central - PubMed

Affiliation: Health Research Institute, Infectious and Tropical Diseases Research Center, Jundishapur University of Medical Sciences, Ahvaz, IR Iran ; Department of Microbiology, Jundishapur University of Medical Sciences, Ahvaz, IR Iran.

ABSTRACT

Background: Neisseria lactamica as one of the main commensal in oropharynx during the childhood is related to the induction of a natural immunity against meningococcal meningitis. Also Moraxella catarrhalis in oropharynx of children is a predisposing factor for otitis media infection.

Objectives: The current study aimed to investigate the frequency of the N. lactamica, other nonpathogenic Neisseria spp. and M. catarrhalis in the oropharynx of young healthy children in Ahvaz, Iran by the two phenotypic tests and Polymerase Chain Reaction (PCR).

Materials and methods: A total of 192 oropharyngeal swab samples of the young healthy children were studied during four months. Swabs were plated onto enriched selective media and non-selective media. Gram-negative and oxidase-positive diplococci were identified by several conventional biochemical tests. The PCR and sequencing were used to confirm the accuracy of laboratory diagnosis to identify N. lactamica and M. catarrhalis.

Results: Among 192 young healthy children with the mean age of 5.93 ± 2.5903 years, authors identified: N. lactamica (21.9%) in the age group of one to nine years; N. mucosa (6.3%); N. sicca (7.8%); N. cinerea (1.6%); N. subflava (biovar subflava) (4.2%); N. subflava (biovar perflava) (28.1%); N. subflava (biovar flava) (7.3%) and M. catarrhalis (42.7%).

Conclusions: The young healthy children screening by colonization of N. lactamica and other nonpathogenic Neisseria spp. in oropharynx was the first report in Ahvaz, Iran. The study results demonstrated the high frequency of colonization of M. catarrhalis in the studied young healthy children other than Neisseria spp.

No MeSH data available.


Related in: MedlinePlus

PCR AmplificationA) glyRS gene of M. catarrhalis strains. Lanes 2-8, isolated strains; lane 9, negative control; lanes 10-11, standard strains; lanes 1 and 12; 50 bp DNA marker. B) pdhC gene of N. lactamica strains. Lane 2, standard strain; lanes 3-6, isolated strains; lanes 7-8, negative controls; lanes 1 and 9, 50 bp DNA marker
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fig16059: PCR AmplificationA) glyRS gene of M. catarrhalis strains. Lanes 2-8, isolated strains; lane 9, negative control; lanes 10-11, standard strains; lanes 1 and 12; 50 bp DNA marker. B) pdhC gene of N. lactamica strains. Lane 2, standard strain; lanes 3-6, isolated strains; lanes 7-8, negative controls; lanes 1 and 9, 50 bp DNA marker


Oropharyngeal Colonization With Neisseria lactamica, Other Nonpathogenic Neisseria Species and Moraxella catarrhalis Among Young Healthy Children in Ahvaz, Iran.

Sheikhi R, Amin M, Rostami S, Shoja S, Ebrahimi N - Jundishapur J Microbiol (2015)

PCR AmplificationA) glyRS gene of M. catarrhalis strains. Lanes 2-8, isolated strains; lane 9, negative control; lanes 10-11, standard strains; lanes 1 and 12; 50 bp DNA marker. B) pdhC gene of N. lactamica strains. Lane 2, standard strain; lanes 3-6, isolated strains; lanes 7-8, negative controls; lanes 1 and 9, 50 bp DNA marker
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4418171&req=5

fig16059: PCR AmplificationA) glyRS gene of M. catarrhalis strains. Lanes 2-8, isolated strains; lane 9, negative control; lanes 10-11, standard strains; lanes 1 and 12; 50 bp DNA marker. B) pdhC gene of N. lactamica strains. Lane 2, standard strain; lanes 3-6, isolated strains; lanes 7-8, negative controls; lanes 1 and 9, 50 bp DNA marker
Bottom Line: The PCR and sequencing were used to confirm the accuracy of laboratory diagnosis to identify N. lactamica and M. catarrhalis.The young healthy children screening by colonization of N. lactamica and other nonpathogenic Neisseria spp. in oropharynx was the first report in Ahvaz, Iran.The study results demonstrated the high frequency of colonization of M. catarrhalis in the studied young healthy children other than Neisseria spp.

View Article: PubMed Central - PubMed

Affiliation: Health Research Institute, Infectious and Tropical Diseases Research Center, Jundishapur University of Medical Sciences, Ahvaz, IR Iran ; Department of Microbiology, Jundishapur University of Medical Sciences, Ahvaz, IR Iran.

ABSTRACT

Background: Neisseria lactamica as one of the main commensal in oropharynx during the childhood is related to the induction of a natural immunity against meningococcal meningitis. Also Moraxella catarrhalis in oropharynx of children is a predisposing factor for otitis media infection.

Objectives: The current study aimed to investigate the frequency of the N. lactamica, other nonpathogenic Neisseria spp. and M. catarrhalis in the oropharynx of young healthy children in Ahvaz, Iran by the two phenotypic tests and Polymerase Chain Reaction (PCR).

Materials and methods: A total of 192 oropharyngeal swab samples of the young healthy children were studied during four months. Swabs were plated onto enriched selective media and non-selective media. Gram-negative and oxidase-positive diplococci were identified by several conventional biochemical tests. The PCR and sequencing were used to confirm the accuracy of laboratory diagnosis to identify N. lactamica and M. catarrhalis.

Results: Among 192 young healthy children with the mean age of 5.93 ± 2.5903 years, authors identified: N. lactamica (21.9%) in the age group of one to nine years; N. mucosa (6.3%); N. sicca (7.8%); N. cinerea (1.6%); N. subflava (biovar subflava) (4.2%); N. subflava (biovar perflava) (28.1%); N. subflava (biovar flava) (7.3%) and M. catarrhalis (42.7%).

Conclusions: The young healthy children screening by colonization of N. lactamica and other nonpathogenic Neisseria spp. in oropharynx was the first report in Ahvaz, Iran. The study results demonstrated the high frequency of colonization of M. catarrhalis in the studied young healthy children other than Neisseria spp.

No MeSH data available.


Related in: MedlinePlus