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Salmonella enterica serovar Weltevreden ST1500 associated foodborne outbreak in Pune, India.

Jain P, Nandy S, Bharadwaj R, Niyogi SK, Dutta S - Indian J. Med. Res. (2015)

View Article: PubMed Central - PubMed

Affiliation: Division of Bacteriology, National Institute of Cholera & Enteric Diseases (NICED), P-33, CIT Road, Scheme XM, Beliaghata, Kolkata, West Bengal, India.

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Weltevreden) has emerged as a dominant foodborne pathogen globally especially in South-East Asian countries, being increasingly isolated from vegetables, meat and seafoods... Weltevreden associated foodborne outbreaks of gastroenteritis have been reported from Scandinavian countries, Réunion Island (France) and India in the past... Weltevreden outbreak strain 2007-60-3289-1 isolated in Scandinavia was available in EMBL nucleotide sequence database, the implicating agent of S... Weltevreden associated outbreaks in India have not been characterized earlier... Weltevreden associated foodborne gastroenteritis outbreak among student staying in a hostel at Pune, Maharashtra, India, and characterization of the isolates by antimicrobial susceptibility pattern, plasmid profiling and typing, pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST)... On January 19, 2010, 150 students aged between 20-30 yr had consumed food comprising milk, rice, sprouted pulses and curd from their hostel canteen for lunch in Pune, Maharashtra... Almost all of them developed acute watery diarrhoea within 12 h of having food, associated with fever, abdominal cramps, nausea and vomiting... Ltd, Tokyo, Japan) and nomenclature was done following Kauffmann White Scheme... The isolates were tested for their antimicrobial susceptibility using 17 antibiotic discs (BD BBL™, Marryland, USA); ampicillin, chloramphenicol, tetracycline, co-trimoxazole, nalidixic acid, ciprofloxacin, norfloxacin, ofloxacin, gentamicin, amikacin, streptomycin, cefotaxime, ceftazidime, ceftriaxone, aztreonam, azithromycin and erythromycin, by disc diffusion method and results were interpreted following CLSI (Clinical and Laboratory Standards Institute) guidelines... All the study isolates (Pune outbreak and Kolkata sporadic) were uniformly susceptible to the 17 antimicrobials tested... The plasmids of type IncFIIS are considered as virulence plasmids in Salmonella, but its role in disease pathogenesis is yet to be established... The Pune outbreak (A) and Kolkata sporadic (B) isolates formed distinct clusters in PFGE (Figure)... All the outbreak isolates were found to be clonal (100%) and had 87 per cent similarity with the sporadic isolates... Weltevreden human isolates from Sri Lanka, Australia, Indonesia, Zimbabwe, Philippines and Germany belonging to ST365 and that of an environmental isolate from UK belonging to ST1500 were available in the aforesaid MLST database.

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Pulsed field gel electrophoresis (PFGE) profiles of XbaI digest of DNA of S. Weltevreden Pune outbreak isolates, 2010 by cluster analysis and comparison with S. Weltevreden Kolkata sporadic isolates. Band comparison was performed using Dice coefficient with 1.50 per cent optimization (Opt) and 1.50 per cent position tolerance (Tol). H, minimum height; S, minimum surface; mo, months; M, male; F, female.
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Figure 1: Pulsed field gel electrophoresis (PFGE) profiles of XbaI digest of DNA of S. Weltevreden Pune outbreak isolates, 2010 by cluster analysis and comparison with S. Weltevreden Kolkata sporadic isolates. Band comparison was performed using Dice coefficient with 1.50 per cent optimization (Opt) and 1.50 per cent position tolerance (Tol). H, minimum height; S, minimum surface; mo, months; M, male; F, female.

Mentions: The Pune outbreak (A) and Kolkata sporadic (B) isolates formed distinct clusters in PFGE (Figure). All the outbreak isolates were found to be clonal (100%) and had 87 per cent similarity with the sporadic isolates. MLST database characterized all the study isolates as ST1500 [Clonal complex (CC) type 205] based on their sequence data. The allelic types of the seven genes were aroC: 130, dnaN: 97, hemD: 25, hisD: 125, purE: 422, sucA: 9, and thrA: 101. The current data have been entered in the MLST database and are available at the website (http://mlst.ucc.ie/mlst/dbs/Senterica/GetTableInfo_html). S. Weltevreden human isolates from Sri Lanka, Australia, Indonesia, Zimbabwe, Philippines and Germany belonging to ST365 and that of an environmental isolate from UK belonging to ST1500 were available in the aforesaid MLST database. This report showed that the foodborne outbreak caused by Indian S. Weltevreden isolates belonged to ST1500 (CC205).


Salmonella enterica serovar Weltevreden ST1500 associated foodborne outbreak in Pune, India.

Jain P, Nandy S, Bharadwaj R, Niyogi SK, Dutta S - Indian J. Med. Res. (2015)

Pulsed field gel electrophoresis (PFGE) profiles of XbaI digest of DNA of S. Weltevreden Pune outbreak isolates, 2010 by cluster analysis and comparison with S. Weltevreden Kolkata sporadic isolates. Band comparison was performed using Dice coefficient with 1.50 per cent optimization (Opt) and 1.50 per cent position tolerance (Tol). H, minimum height; S, minimum surface; mo, months; M, male; F, female.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
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getmorefigures.php?uid=PMC4418162&req=5

Figure 1: Pulsed field gel electrophoresis (PFGE) profiles of XbaI digest of DNA of S. Weltevreden Pune outbreak isolates, 2010 by cluster analysis and comparison with S. Weltevreden Kolkata sporadic isolates. Band comparison was performed using Dice coefficient with 1.50 per cent optimization (Opt) and 1.50 per cent position tolerance (Tol). H, minimum height; S, minimum surface; mo, months; M, male; F, female.
Mentions: The Pune outbreak (A) and Kolkata sporadic (B) isolates formed distinct clusters in PFGE (Figure). All the outbreak isolates were found to be clonal (100%) and had 87 per cent similarity with the sporadic isolates. MLST database characterized all the study isolates as ST1500 [Clonal complex (CC) type 205] based on their sequence data. The allelic types of the seven genes were aroC: 130, dnaN: 97, hemD: 25, hisD: 125, purE: 422, sucA: 9, and thrA: 101. The current data have been entered in the MLST database and are available at the website (http://mlst.ucc.ie/mlst/dbs/Senterica/GetTableInfo_html). S. Weltevreden human isolates from Sri Lanka, Australia, Indonesia, Zimbabwe, Philippines and Germany belonging to ST365 and that of an environmental isolate from UK belonging to ST1500 were available in the aforesaid MLST database. This report showed that the foodborne outbreak caused by Indian S. Weltevreden isolates belonged to ST1500 (CC205).

View Article: PubMed Central - PubMed

Affiliation: Division of Bacteriology, National Institute of Cholera & Enteric Diseases (NICED), P-33, CIT Road, Scheme XM, Beliaghata, Kolkata, West Bengal, India.

AUTOMATICALLY GENERATED EXCERPT
Please rate it.

Weltevreden) has emerged as a dominant foodborne pathogen globally especially in South-East Asian countries, being increasingly isolated from vegetables, meat and seafoods... Weltevreden associated foodborne outbreaks of gastroenteritis have been reported from Scandinavian countries, Réunion Island (France) and India in the past... Weltevreden outbreak strain 2007-60-3289-1 isolated in Scandinavia was available in EMBL nucleotide sequence database, the implicating agent of S... Weltevreden associated outbreaks in India have not been characterized earlier... Weltevreden associated foodborne gastroenteritis outbreak among student staying in a hostel at Pune, Maharashtra, India, and characterization of the isolates by antimicrobial susceptibility pattern, plasmid profiling and typing, pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST)... On January 19, 2010, 150 students aged between 20-30 yr had consumed food comprising milk, rice, sprouted pulses and curd from their hostel canteen for lunch in Pune, Maharashtra... Almost all of them developed acute watery diarrhoea within 12 h of having food, associated with fever, abdominal cramps, nausea and vomiting... Ltd, Tokyo, Japan) and nomenclature was done following Kauffmann White Scheme... The isolates were tested for their antimicrobial susceptibility using 17 antibiotic discs (BD BBL™, Marryland, USA); ampicillin, chloramphenicol, tetracycline, co-trimoxazole, nalidixic acid, ciprofloxacin, norfloxacin, ofloxacin, gentamicin, amikacin, streptomycin, cefotaxime, ceftazidime, ceftriaxone, aztreonam, azithromycin and erythromycin, by disc diffusion method and results were interpreted following CLSI (Clinical and Laboratory Standards Institute) guidelines... All the study isolates (Pune outbreak and Kolkata sporadic) were uniformly susceptible to the 17 antimicrobials tested... The plasmids of type IncFIIS are considered as virulence plasmids in Salmonella, but its role in disease pathogenesis is yet to be established... The Pune outbreak (A) and Kolkata sporadic (B) isolates formed distinct clusters in PFGE (Figure)... All the outbreak isolates were found to be clonal (100%) and had 87 per cent similarity with the sporadic isolates... Weltevreden human isolates from Sri Lanka, Australia, Indonesia, Zimbabwe, Philippines and Germany belonging to ST365 and that of an environmental isolate from UK belonging to ST1500 were available in the aforesaid MLST database.

No MeSH data available.