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Anticancer activity of Cynodon dactylon L. root extract against diethyl nitrosamine induced hepatic carcinoma.

Kowsalya R, Kaliaperumal J, Vaishnavi M, Namasivayam E - South Asian J Cancer (2015 Apr-Jun)

Bottom Line: The liver marker enzymes AST and ALT produced significant results in the protective action.These results concluded that enhanced levels of antioxidant enzyme and reduced amount of serum amino transaminase, which are suggested to be the major mechanisms of C. dactylon root extract in protecting the mice from hepatocarcinoma induced by DEN.The methanolic extract of C. dactylon possesses significant anticancer properties.

View Article: PubMed Central - PubMed

Affiliation: Department of Biotechnology, Molecular Pharmacology Research Laboratory, Periyar University, Salem, Tamil Nadu, India.

ABSTRACT

Background: Hepatocellular carcinoma is one of the most common cancers and a lethal disease. In view of the limited treatment and a grave prognosis of liver cancer, preventive control has been emphasized.

Materials and methods: The methanolic extract of roots of Cynodon dactylon was screened for its hepato-protective activity in diethyl nitrosamine (DEN) induced liver cancer in Swiss albino mice. The plant extract at a dose of 50 mg/kg was administered orally once a week, up to 30 days after DEN administration. The animals were sacrificed; blood sample and liver tissue were collected and used for enzyme assay such as, asparatate amino transferase (AST), alanine aminotransferase (ALT), catalase (CAT), glutathione peroxidase (GPx) and glutathione-S-transferase (GST). The liver marker enzymes AST and ALT produced significant results in the protective action.

Results: The antioxidant enzyme assay results concerning the improved activity of GPx, GST and CAT. These results concluded that enhanced levels of antioxidant enzyme and reduced amount of serum amino transaminase, which are suggested to be the major mechanisms of C. dactylon root extract in protecting the mice from hepatocarcinoma induced by DEN. These biochemical observations were supplemented by histopathological examination of liver sections.

Conclusion: The methanolic extract of C. dactylon possesses significant anticancer properties.

No MeSH data available.


Related in: MedlinePlus

Swiss albino mice were treated with diethyl nitrosamine (20 mg/kg) and/or crude plant extract of Cynodon dactylon 50 mg/kg and tamoxifen 20 mg/kg. Saline treated animal served as control. Glutathione peroxidase values are expressed as mean ± standard deviation, n = 6 in each group, **P < 0.01, *P < 0.05 compared with control groups
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Figure 4: Swiss albino mice were treated with diethyl nitrosamine (20 mg/kg) and/or crude plant extract of Cynodon dactylon 50 mg/kg and tamoxifen 20 mg/kg. Saline treated animal served as control. Glutathione peroxidase values are expressed as mean ± standard deviation, n = 6 in each group, **P < 0.01, *P < 0.05 compared with control groups

Mentions: Diethyl nitrosamine treated group showed low significant elevation (P < 0.05) in liver GST activity with respect to control, Whereas the control, DEN + C. dactylon, DEN + Tamoxifen treated animals did not shown any alteration [Figure 3]. A highly significant (P < 0.01) elevation in GPx activity was observed in DEN treated mice, whereas DEN + C. dactylon showed low significant alteration the same time saline and DEN + Tamoxifen treated animals did not show any significant alteration [Figure 4]. DEN, DEN + C. dactylon showed low significant depletion (P < 0.05) in liver CAT activity with respect to control, whereas saline and DEN + Tamoxifen treated animals did not showed any significant alteration [Figure 5].


Anticancer activity of Cynodon dactylon L. root extract against diethyl nitrosamine induced hepatic carcinoma.

Kowsalya R, Kaliaperumal J, Vaishnavi M, Namasivayam E - South Asian J Cancer (2015 Apr-Jun)

Swiss albino mice were treated with diethyl nitrosamine (20 mg/kg) and/or crude plant extract of Cynodon dactylon 50 mg/kg and tamoxifen 20 mg/kg. Saline treated animal served as control. Glutathione peroxidase values are expressed as mean ± standard deviation, n = 6 in each group, **P < 0.01, *P < 0.05 compared with control groups
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4418089&req=5

Figure 4: Swiss albino mice were treated with diethyl nitrosamine (20 mg/kg) and/or crude plant extract of Cynodon dactylon 50 mg/kg and tamoxifen 20 mg/kg. Saline treated animal served as control. Glutathione peroxidase values are expressed as mean ± standard deviation, n = 6 in each group, **P < 0.01, *P < 0.05 compared with control groups
Mentions: Diethyl nitrosamine treated group showed low significant elevation (P < 0.05) in liver GST activity with respect to control, Whereas the control, DEN + C. dactylon, DEN + Tamoxifen treated animals did not shown any alteration [Figure 3]. A highly significant (P < 0.01) elevation in GPx activity was observed in DEN treated mice, whereas DEN + C. dactylon showed low significant alteration the same time saline and DEN + Tamoxifen treated animals did not show any significant alteration [Figure 4]. DEN, DEN + C. dactylon showed low significant depletion (P < 0.05) in liver CAT activity with respect to control, whereas saline and DEN + Tamoxifen treated animals did not showed any significant alteration [Figure 5].

Bottom Line: The liver marker enzymes AST and ALT produced significant results in the protective action.These results concluded that enhanced levels of antioxidant enzyme and reduced amount of serum amino transaminase, which are suggested to be the major mechanisms of C. dactylon root extract in protecting the mice from hepatocarcinoma induced by DEN.The methanolic extract of C. dactylon possesses significant anticancer properties.

View Article: PubMed Central - PubMed

Affiliation: Department of Biotechnology, Molecular Pharmacology Research Laboratory, Periyar University, Salem, Tamil Nadu, India.

ABSTRACT

Background: Hepatocellular carcinoma is one of the most common cancers and a lethal disease. In view of the limited treatment and a grave prognosis of liver cancer, preventive control has been emphasized.

Materials and methods: The methanolic extract of roots of Cynodon dactylon was screened for its hepato-protective activity in diethyl nitrosamine (DEN) induced liver cancer in Swiss albino mice. The plant extract at a dose of 50 mg/kg was administered orally once a week, up to 30 days after DEN administration. The animals were sacrificed; blood sample and liver tissue were collected and used for enzyme assay such as, asparatate amino transferase (AST), alanine aminotransferase (ALT), catalase (CAT), glutathione peroxidase (GPx) and glutathione-S-transferase (GST). The liver marker enzymes AST and ALT produced significant results in the protective action.

Results: The antioxidant enzyme assay results concerning the improved activity of GPx, GST and CAT. These results concluded that enhanced levels of antioxidant enzyme and reduced amount of serum amino transaminase, which are suggested to be the major mechanisms of C. dactylon root extract in protecting the mice from hepatocarcinoma induced by DEN. These biochemical observations were supplemented by histopathological examination of liver sections.

Conclusion: The methanolic extract of C. dactylon possesses significant anticancer properties.

No MeSH data available.


Related in: MedlinePlus