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Cytoprotective and antioxidant effects of Echium amoenum anthocyanin-rich extract in human endothelial cells (HUVECs).

Safaeian L, Haghjoo Javanmard S, Ghanadian M, Seifabadi S - Avicenna J Phytomed (2015 Mar-Apr)

Bottom Line: Moreover, it did not show cytotoxic effects at the concentration range of 25-1000 µg/ml.These results suggest antioxidant and protective effect of anthocyanin-rich extract of the petals of E. amoenum against H2O2-induced oxidative stress in HUVECs.However, further investigations are needed for understanding the detailed mechanisms of cytoprotective effects of this traditional herbal medicine.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology and Toxicology and Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences , Isfahan , Iran.

ABSTRACT

Objective: Echium amoenum Fisch. & C.A. Mey. is used for the treatment of various diseases in traditional medicine. This plant is a major source of anthocyanins with beneficial cardiovascular properties such as anti-atherosclerotic and antihypertensive effects. In the present study, the protective and antioxidant effects of anthocyanin-rich E. amoenum extract were evaluated on human vascular endothelial cells (HUVECs) under oxidative stress.

Materials and methods: Cell viability and oxidative status were assessed on H2O2-induced oxidative stress (0.5 mM H2O2 for 2 h) in HUVECs pretreated by anthocyanin-rich extract from the petals of E. amoenum (25-1000 µg/ml). Cytoprotective effect of the extract was evaluated by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The hydroperoxides concentration and ferric reducing antioxidant power (FRAP) were assessed in intra- and extra-cellular fluid of pretreated cells.

Results: Pretreatment of HUVECs with E. amoenum extract at the concentrations of 100-1000 µg/ml reduced the cell death resulted from the exposure to H2O2 in a concentration-dependent manner. E. amoenum extract decreased hydroperoxides concentration and increased FRAP value in both intra- and extra-cellular fluid at different concentration ranges. Moreover, it did not show cytotoxic effects at the concentration range of 25-1000 µg/ml.

Conclusion: These results suggest antioxidant and protective effect of anthocyanin-rich extract of the petals of E. amoenum against H2O2-induced oxidative stress in HUVECs. However, further investigations are needed for understanding the detailed mechanisms of cytoprotective effects of this traditional herbal medicine.

No MeSH data available.


Related in: MedlinePlus

Effect of E. amoenum extract on H2O2-induced oxidative stress in HUVECs. Cells were incubated with H2O2 (0.5 mM, 2h) after pretreatment with different concentrations of E. amoenum extract (25-1000 μg/ml). The cell viability was determined using the MTT assay. Values are means+SEM from three independent experiments in triplicate. ###p<0.001 versus control (untreated cells), ***p<0.001 versus H2O2 stimulated cells.
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Figure 2: Effect of E. amoenum extract on H2O2-induced oxidative stress in HUVECs. Cells were incubated with H2O2 (0.5 mM, 2h) after pretreatment with different concentrations of E. amoenum extract (25-1000 μg/ml). The cell viability was determined using the MTT assay. Values are means+SEM from three independent experiments in triplicate. ###p<0.001 versus control (untreated cells), ***p<0.001 versus H2O2 stimulated cells.

Mentions: Figure 2 shows the cytoprotective effect of E. amoenum against oxidative stress-induced cell injury on HUVECs using the MTT method. The exposure of HUVECs to 0.5 mM H2O2 for 2 h resulted in a significant reduction in cell viability (p<0.001).


Cytoprotective and antioxidant effects of Echium amoenum anthocyanin-rich extract in human endothelial cells (HUVECs).

Safaeian L, Haghjoo Javanmard S, Ghanadian M, Seifabadi S - Avicenna J Phytomed (2015 Mar-Apr)

Effect of E. amoenum extract on H2O2-induced oxidative stress in HUVECs. Cells were incubated with H2O2 (0.5 mM, 2h) after pretreatment with different concentrations of E. amoenum extract (25-1000 μg/ml). The cell viability was determined using the MTT assay. Values are means+SEM from three independent experiments in triplicate. ###p<0.001 versus control (untreated cells), ***p<0.001 versus H2O2 stimulated cells.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4418065&req=5

Figure 2: Effect of E. amoenum extract on H2O2-induced oxidative stress in HUVECs. Cells were incubated with H2O2 (0.5 mM, 2h) after pretreatment with different concentrations of E. amoenum extract (25-1000 μg/ml). The cell viability was determined using the MTT assay. Values are means+SEM from three independent experiments in triplicate. ###p<0.001 versus control (untreated cells), ***p<0.001 versus H2O2 stimulated cells.
Mentions: Figure 2 shows the cytoprotective effect of E. amoenum against oxidative stress-induced cell injury on HUVECs using the MTT method. The exposure of HUVECs to 0.5 mM H2O2 for 2 h resulted in a significant reduction in cell viability (p<0.001).

Bottom Line: Moreover, it did not show cytotoxic effects at the concentration range of 25-1000 µg/ml.These results suggest antioxidant and protective effect of anthocyanin-rich extract of the petals of E. amoenum against H2O2-induced oxidative stress in HUVECs.However, further investigations are needed for understanding the detailed mechanisms of cytoprotective effects of this traditional herbal medicine.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology and Toxicology and Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences , Isfahan , Iran.

ABSTRACT

Objective: Echium amoenum Fisch. & C.A. Mey. is used for the treatment of various diseases in traditional medicine. This plant is a major source of anthocyanins with beneficial cardiovascular properties such as anti-atherosclerotic and antihypertensive effects. In the present study, the protective and antioxidant effects of anthocyanin-rich E. amoenum extract were evaluated on human vascular endothelial cells (HUVECs) under oxidative stress.

Materials and methods: Cell viability and oxidative status were assessed on H2O2-induced oxidative stress (0.5 mM H2O2 for 2 h) in HUVECs pretreated by anthocyanin-rich extract from the petals of E. amoenum (25-1000 µg/ml). Cytoprotective effect of the extract was evaluated by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The hydroperoxides concentration and ferric reducing antioxidant power (FRAP) were assessed in intra- and extra-cellular fluid of pretreated cells.

Results: Pretreatment of HUVECs with E. amoenum extract at the concentrations of 100-1000 µg/ml reduced the cell death resulted from the exposure to H2O2 in a concentration-dependent manner. E. amoenum extract decreased hydroperoxides concentration and increased FRAP value in both intra- and extra-cellular fluid at different concentration ranges. Moreover, it did not show cytotoxic effects at the concentration range of 25-1000 µg/ml.

Conclusion: These results suggest antioxidant and protective effect of anthocyanin-rich extract of the petals of E. amoenum against H2O2-induced oxidative stress in HUVECs. However, further investigations are needed for understanding the detailed mechanisms of cytoprotective effects of this traditional herbal medicine.

No MeSH data available.


Related in: MedlinePlus