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Evaluation of the antibacterial activity of the Althaea officinalis L. leaf extract and its wound healing potency in the rat model of excision wound creation.

Rezaei M, Dadgar Z, Noori-Zadeh A, Mesbah-Namin SA, Pakzad I, Davodian E - Avicenna J Phytomed (2015 Mar-Apr)

Bottom Line: Plants and their extracts known as phytomedicine have immense potential for the management and treatment of wounds.The extract was also tested in the form of topical administration on excision wound model in rats.In the extract-treated wounds, the wound healing percent was significantly increased in comparison with controls.

View Article: PubMed Central - PubMed

Affiliation: Researcher, Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khoramabad, Iran.

ABSTRACT

Objectives: Wound is defined simply as the disruption of the biochemical, cellular, and anatomic continuity of a tissue. Plants and their extracts known as phytomedicine have immense potential for the management and treatment of wounds.

Materials and methods: Due to the undesirable side effects, in the control and treatment of the wound infections, it is recommended to use natural materials such as phytochemicals instead of chemically synthesized drugs. Thus, the aim of this research was to study the anti-microbial and wound healing potential of Althaea officinalis L. hydroalchoholic extract in comparison with ciprofloxacin, gentamicin, and penicillin antibiotics on clinical strains as well as pathogenic bacteria such as Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, and Listeria monocytogenes under in vitro conditions using micro broth dilution and disc diffusion methods. Moreover, MIC and MBC of its hydroalchoholic extract was also evaluated.

Results: The results showed that although Althaea officinalis L. extract was not effective on gram-negative bacteria but it was efficacious on gram-positive bacteria. The extract was also tested in the form of topical administration on excision wound model in rats. In the extract-treated wounds, the wound healing percent was significantly increased in comparison with controls.

Conclusions: Based on this research, herbal extract of officinalis L. can be a great candidate for the treatment of gram-positive infections and merits further studies.

No MeSH data available.


Related in: MedlinePlus

Immunocytochemistry photomicrographs of wound repair at different times of treatments in the three groups of rat model of excision wound creation A, B, and C) Wound healing after 7 days of treatment in the sham, zinc oxide, and Althaea officinalis L. hydroethanolic extract-treated groups, respectively. D, E, and F) Wound healing after 14 days in the sham, zinc oxide, and Althaea officinalis L. hydroethanolic extract-treated groups, respectively. G, H, and I) Wound healing after 21 days in the sham, zinc oxide, and Althaea officinalis L. hydroethanolic extract-treated groups.
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Figure 2: Immunocytochemistry photomicrographs of wound repair at different times of treatments in the three groups of rat model of excision wound creation A, B, and C) Wound healing after 7 days of treatment in the sham, zinc oxide, and Althaea officinalis L. hydroethanolic extract-treated groups, respectively. D, E, and F) Wound healing after 14 days in the sham, zinc oxide, and Althaea officinalis L. hydroethanolic extract-treated groups, respectively. G, H, and I) Wound healing after 21 days in the sham, zinc oxide, and Althaea officinalis L. hydroethanolic extract-treated groups.

Mentions: The results showed matrix irregularity of the connective tissue and clear inflammatory reactions compared to the group receiving Althaea officinalis hydroalchoholic extract. Sham group had an irregular granulated tissue, more cells, and high inflammation. On 21th day of the study in Althaea officinalis hydroalchoholic extract-treated group, the cellular density of fibrosis tissue was low, inflammation was brief, and granulation was mature in comparison with the group which received zinc oxide ointment as the scar area in zinc oxide-treated group was big and irregular but epidermal progress was consistent with Althaea officinalis extract-treated group. We didn't observe dermal tissue re-organization differences elements in these two groups. At the end of the treatment process, the epidermis formation was intact in both groups and the scar area was smaller as well. From the pathological aspects, the tissue samples treated with Althaea officinalis extract had better healing quality with regular arrangement and low inflammation density and time needed for repairing (Figure 2).


Evaluation of the antibacterial activity of the Althaea officinalis L. leaf extract and its wound healing potency in the rat model of excision wound creation.

Rezaei M, Dadgar Z, Noori-Zadeh A, Mesbah-Namin SA, Pakzad I, Davodian E - Avicenna J Phytomed (2015 Mar-Apr)

Immunocytochemistry photomicrographs of wound repair at different times of treatments in the three groups of rat model of excision wound creation A, B, and C) Wound healing after 7 days of treatment in the sham, zinc oxide, and Althaea officinalis L. hydroethanolic extract-treated groups, respectively. D, E, and F) Wound healing after 14 days in the sham, zinc oxide, and Althaea officinalis L. hydroethanolic extract-treated groups, respectively. G, H, and I) Wound healing after 21 days in the sham, zinc oxide, and Althaea officinalis L. hydroethanolic extract-treated groups.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4418059&req=5

Figure 2: Immunocytochemistry photomicrographs of wound repair at different times of treatments in the three groups of rat model of excision wound creation A, B, and C) Wound healing after 7 days of treatment in the sham, zinc oxide, and Althaea officinalis L. hydroethanolic extract-treated groups, respectively. D, E, and F) Wound healing after 14 days in the sham, zinc oxide, and Althaea officinalis L. hydroethanolic extract-treated groups, respectively. G, H, and I) Wound healing after 21 days in the sham, zinc oxide, and Althaea officinalis L. hydroethanolic extract-treated groups.
Mentions: The results showed matrix irregularity of the connective tissue and clear inflammatory reactions compared to the group receiving Althaea officinalis hydroalchoholic extract. Sham group had an irregular granulated tissue, more cells, and high inflammation. On 21th day of the study in Althaea officinalis hydroalchoholic extract-treated group, the cellular density of fibrosis tissue was low, inflammation was brief, and granulation was mature in comparison with the group which received zinc oxide ointment as the scar area in zinc oxide-treated group was big and irregular but epidermal progress was consistent with Althaea officinalis extract-treated group. We didn't observe dermal tissue re-organization differences elements in these two groups. At the end of the treatment process, the epidermis formation was intact in both groups and the scar area was smaller as well. From the pathological aspects, the tissue samples treated with Althaea officinalis extract had better healing quality with regular arrangement and low inflammation density and time needed for repairing (Figure 2).

Bottom Line: Plants and their extracts known as phytomedicine have immense potential for the management and treatment of wounds.The extract was also tested in the form of topical administration on excision wound model in rats.In the extract-treated wounds, the wound healing percent was significantly increased in comparison with controls.

View Article: PubMed Central - PubMed

Affiliation: Researcher, Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khoramabad, Iran.

ABSTRACT

Objectives: Wound is defined simply as the disruption of the biochemical, cellular, and anatomic continuity of a tissue. Plants and their extracts known as phytomedicine have immense potential for the management and treatment of wounds.

Materials and methods: Due to the undesirable side effects, in the control and treatment of the wound infections, it is recommended to use natural materials such as phytochemicals instead of chemically synthesized drugs. Thus, the aim of this research was to study the anti-microbial and wound healing potential of Althaea officinalis L. hydroalchoholic extract in comparison with ciprofloxacin, gentamicin, and penicillin antibiotics on clinical strains as well as pathogenic bacteria such as Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, and Listeria monocytogenes under in vitro conditions using micro broth dilution and disc diffusion methods. Moreover, MIC and MBC of its hydroalchoholic extract was also evaluated.

Results: The results showed that although Althaea officinalis L. extract was not effective on gram-negative bacteria but it was efficacious on gram-positive bacteria. The extract was also tested in the form of topical administration on excision wound model in rats. In the extract-treated wounds, the wound healing percent was significantly increased in comparison with controls.

Conclusions: Based on this research, herbal extract of officinalis L. can be a great candidate for the treatment of gram-positive infections and merits further studies.

No MeSH data available.


Related in: MedlinePlus