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Development of a method to detect three monohydroxylated polycyclic aromatic hydrocarbons in human urine by liquid chromatographic tandem mass spectrometry.

Zhang X, Hou H, Xiong W, Hu Q - J Anal Methods Chem (2015)

Bottom Line: The method has good accuracy (72.1-107.7%) and reproducibility (1.8-11.4%) and was successfully used to study the uptake of pyrene, benzo[a]pyrene, and benzo[a]anthracene from cigarette smoke.The results indicated that urinary 1-OHP concentration in the smoking group (66.58 ± 70.91 ng/g creatinine) was higher than that observed in the nonsmoking group (58.16 ± 49.48 ng/g creatinine).Urinary 1-OHP levels exhibited a significant correlation with BaP yield in cigarette smoke under the Canadian intense smoking condition (y = 3.5563x + 30.171, R (2) = 0.9916, n = 227).

View Article: PubMed Central - PubMed

Affiliation: China National Tobacco Quality Supervision & Test Center, No. 2 Fengyang Street, Zhengzhou, Henan 450001, China.

ABSTRACT
A liquid chromatographic tandem mass spectrometry method (LC-MS/MS) for the simultaneous determination of 1-hydroxypyrene (1-OHP), 3-hydroxybenzo[a]pyrene (3-OHBaP), and 3-hydroxybenz[a]anthracene (3-OHBaA) in human urine has been developed. With the exception of 3-OHBaP at a low spiking level, the average recoveries were greater than 80%. The method has good accuracy (72.1-107.7%) and reproducibility (1.8-11.4%) and was successfully used to study the uptake of pyrene, benzo[a]pyrene, and benzo[a]anthracene from cigarette smoke. The results indicated that urinary 1-OHP concentration in the smoking group (66.58 ± 70.91 ng/g creatinine) was higher than that observed in the nonsmoking group (58.16 ± 49.48 ng/g creatinine). Urinary 3-OHBaA concentrations in nonsmokers and smokers with 8 mg and 10 mg tar cigarettes were 10.98 ± 4.39 ng/g creatinine, 11.01 ± 13.30 ng/g creatinine, and 9.17 ± 12.89 ng/g creatinine, respectively. Urinary 3-OHBaP concentrations in nonsmokers and smokers with 8 mg and 13 mg tar cigarettes were 1.30 ± 0.20 ng/g creatinine, 2.83 ± 1.78 ng/g creatinine, and 6.00 ± 4.44 ng/g creatinine, respectively. Urinary 1-OHP levels exhibited a significant correlation with BaP yield in cigarette smoke under the Canadian intense smoking condition (y = 3.5563x + 30.171, R (2) = 0.9916, n = 227).

No MeSH data available.


Correlation between urinary cotinine and 1-OHP.
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Related In: Results  -  Collection


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fig4: Correlation between urinary cotinine and 1-OHP.

Mentions: Because spot urine sampling may not provide a valid overview of the entire toxicant exposure profile [38] and is easily influenced by other factors (e.g., diet), urinary 1-OHP, 3-OHBaA, and 3-OHBaP concentrations were adjusted by creatinine excreted in urine at a relatively constant rate through glomerular filtration. The results after the creatinine correction are summarized in Table 4. Urinary 1-OHP and 3-OHBaP levels increased with tar content, while 3-OHBaA levels did not. Significant differences were observed in the urinary 1-OHP levels between smokers with 8 mg tar cigarettes and 10 mg tar cigarettes (P < 0.01) and between smokers with 10 mg tar cigarettes and 13 mg tar cigarettes (P < 0.05). However, the differences in urinary 3-OHBaA and 3-OHBaP between different groups of smokers were not significant (P > 0.05). The correlation between 1-OHP in 24 h urine and the nicotine metabolite cotinine, which is a recognized biomarker for tobacco exposure, was also studied (Figure 4). The correlation between urinary 1-OHP and cotinine was weak (y = 1.7932x + 571.31, R2 = 0.0256).


Development of a method to detect three monohydroxylated polycyclic aromatic hydrocarbons in human urine by liquid chromatographic tandem mass spectrometry.

Zhang X, Hou H, Xiong W, Hu Q - J Anal Methods Chem (2015)

Correlation between urinary cotinine and 1-OHP.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4418005&req=5

fig4: Correlation between urinary cotinine and 1-OHP.
Mentions: Because spot urine sampling may not provide a valid overview of the entire toxicant exposure profile [38] and is easily influenced by other factors (e.g., diet), urinary 1-OHP, 3-OHBaA, and 3-OHBaP concentrations were adjusted by creatinine excreted in urine at a relatively constant rate through glomerular filtration. The results after the creatinine correction are summarized in Table 4. Urinary 1-OHP and 3-OHBaP levels increased with tar content, while 3-OHBaA levels did not. Significant differences were observed in the urinary 1-OHP levels between smokers with 8 mg tar cigarettes and 10 mg tar cigarettes (P < 0.01) and between smokers with 10 mg tar cigarettes and 13 mg tar cigarettes (P < 0.05). However, the differences in urinary 3-OHBaA and 3-OHBaP between different groups of smokers were not significant (P > 0.05). The correlation between 1-OHP in 24 h urine and the nicotine metabolite cotinine, which is a recognized biomarker for tobacco exposure, was also studied (Figure 4). The correlation between urinary 1-OHP and cotinine was weak (y = 1.7932x + 571.31, R2 = 0.0256).

Bottom Line: The method has good accuracy (72.1-107.7%) and reproducibility (1.8-11.4%) and was successfully used to study the uptake of pyrene, benzo[a]pyrene, and benzo[a]anthracene from cigarette smoke.The results indicated that urinary 1-OHP concentration in the smoking group (66.58 ± 70.91 ng/g creatinine) was higher than that observed in the nonsmoking group (58.16 ± 49.48 ng/g creatinine).Urinary 1-OHP levels exhibited a significant correlation with BaP yield in cigarette smoke under the Canadian intense smoking condition (y = 3.5563x + 30.171, R (2) = 0.9916, n = 227).

View Article: PubMed Central - PubMed

Affiliation: China National Tobacco Quality Supervision & Test Center, No. 2 Fengyang Street, Zhengzhou, Henan 450001, China.

ABSTRACT
A liquid chromatographic tandem mass spectrometry method (LC-MS/MS) for the simultaneous determination of 1-hydroxypyrene (1-OHP), 3-hydroxybenzo[a]pyrene (3-OHBaP), and 3-hydroxybenz[a]anthracene (3-OHBaA) in human urine has been developed. With the exception of 3-OHBaP at a low spiking level, the average recoveries were greater than 80%. The method has good accuracy (72.1-107.7%) and reproducibility (1.8-11.4%) and was successfully used to study the uptake of pyrene, benzo[a]pyrene, and benzo[a]anthracene from cigarette smoke. The results indicated that urinary 1-OHP concentration in the smoking group (66.58 ± 70.91 ng/g creatinine) was higher than that observed in the nonsmoking group (58.16 ± 49.48 ng/g creatinine). Urinary 3-OHBaA concentrations in nonsmokers and smokers with 8 mg and 10 mg tar cigarettes were 10.98 ± 4.39 ng/g creatinine, 11.01 ± 13.30 ng/g creatinine, and 9.17 ± 12.89 ng/g creatinine, respectively. Urinary 3-OHBaP concentrations in nonsmokers and smokers with 8 mg and 13 mg tar cigarettes were 1.30 ± 0.20 ng/g creatinine, 2.83 ± 1.78 ng/g creatinine, and 6.00 ± 4.44 ng/g creatinine, respectively. Urinary 1-OHP levels exhibited a significant correlation with BaP yield in cigarette smoke under the Canadian intense smoking condition (y = 3.5563x + 30.171, R (2) = 0.9916, n = 227).

No MeSH data available.