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Development of a method to detect three monohydroxylated polycyclic aromatic hydrocarbons in human urine by liquid chromatographic tandem mass spectrometry.

Zhang X, Hou H, Xiong W, Hu Q - J Anal Methods Chem (2015)

Bottom Line: The method has good accuracy (72.1-107.7%) and reproducibility (1.8-11.4%) and was successfully used to study the uptake of pyrene, benzo[a]pyrene, and benzo[a]anthracene from cigarette smoke.The results indicated that urinary 1-OHP concentration in the smoking group (66.58 ± 70.91 ng/g creatinine) was higher than that observed in the nonsmoking group (58.16 ± 49.48 ng/g creatinine).Urinary 1-OHP levels exhibited a significant correlation with BaP yield in cigarette smoke under the Canadian intense smoking condition (y = 3.5563x + 30.171, R (2) = 0.9916, n = 227).

View Article: PubMed Central - PubMed

Affiliation: China National Tobacco Quality Supervision & Test Center, No. 2 Fengyang Street, Zhengzhou, Henan 450001, China.

ABSTRACT
A liquid chromatographic tandem mass spectrometry method (LC-MS/MS) for the simultaneous determination of 1-hydroxypyrene (1-OHP), 3-hydroxybenzo[a]pyrene (3-OHBaP), and 3-hydroxybenz[a]anthracene (3-OHBaA) in human urine has been developed. With the exception of 3-OHBaP at a low spiking level, the average recoveries were greater than 80%. The method has good accuracy (72.1-107.7%) and reproducibility (1.8-11.4%) and was successfully used to study the uptake of pyrene, benzo[a]pyrene, and benzo[a]anthracene from cigarette smoke. The results indicated that urinary 1-OHP concentration in the smoking group (66.58 ± 70.91 ng/g creatinine) was higher than that observed in the nonsmoking group (58.16 ± 49.48 ng/g creatinine). Urinary 3-OHBaA concentrations in nonsmokers and smokers with 8 mg and 10 mg tar cigarettes were 10.98 ± 4.39 ng/g creatinine, 11.01 ± 13.30 ng/g creatinine, and 9.17 ± 12.89 ng/g creatinine, respectively. Urinary 3-OHBaP concentrations in nonsmokers and smokers with 8 mg and 13 mg tar cigarettes were 1.30 ± 0.20 ng/g creatinine, 2.83 ± 1.78 ng/g creatinine, and 6.00 ± 4.44 ng/g creatinine, respectively. Urinary 1-OHP levels exhibited a significant correlation with BaP yield in cigarette smoke under the Canadian intense smoking condition (y = 3.5563x + 30.171, R (2) = 0.9916, n = 227).

No MeSH data available.


Urinary 1-OHP, 3-OHBaA, and 3-OHBaP concentrations from nonsmokers and smokers.
© Copyright Policy - open-access
Related In: Results  -  Collection


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fig3: Urinary 1-OHP, 3-OHBaA, and 3-OHBaP concentrations from nonsmokers and smokers.

Mentions: The proposed method was successfully used to analyze 58 urine samples from 58 nonsmokers and 243 urine samples from 81 smokers (smokers smoked 8 mg, 10 mg, and 13 mg tar cigarettes in three different weeks, and urine samples were then collected every 24 h); the results are shown in Figure 3. After removing the outliers and the values lower than the LOQs from each group, the final results were summarized as geometric means (GMs) and geometric standard deviations (GSDs) (Table 3). With the exceptions of urinary 3-OHBaA in smokers with 13 mg tar cigarettes and 3-OHBaP in smokers with 10 mg tar cigarettes, urinary 1-OHP, 3-OHBaA, and 3-OHBaP levels in smokers were higher than those in nonsmokers.


Development of a method to detect three monohydroxylated polycyclic aromatic hydrocarbons in human urine by liquid chromatographic tandem mass spectrometry.

Zhang X, Hou H, Xiong W, Hu Q - J Anal Methods Chem (2015)

Urinary 1-OHP, 3-OHBaA, and 3-OHBaP concentrations from nonsmokers and smokers.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4418005&req=5

fig3: Urinary 1-OHP, 3-OHBaA, and 3-OHBaP concentrations from nonsmokers and smokers.
Mentions: The proposed method was successfully used to analyze 58 urine samples from 58 nonsmokers and 243 urine samples from 81 smokers (smokers smoked 8 mg, 10 mg, and 13 mg tar cigarettes in three different weeks, and urine samples were then collected every 24 h); the results are shown in Figure 3. After removing the outliers and the values lower than the LOQs from each group, the final results were summarized as geometric means (GMs) and geometric standard deviations (GSDs) (Table 3). With the exceptions of urinary 3-OHBaA in smokers with 13 mg tar cigarettes and 3-OHBaP in smokers with 10 mg tar cigarettes, urinary 1-OHP, 3-OHBaA, and 3-OHBaP levels in smokers were higher than those in nonsmokers.

Bottom Line: The method has good accuracy (72.1-107.7%) and reproducibility (1.8-11.4%) and was successfully used to study the uptake of pyrene, benzo[a]pyrene, and benzo[a]anthracene from cigarette smoke.The results indicated that urinary 1-OHP concentration in the smoking group (66.58 ± 70.91 ng/g creatinine) was higher than that observed in the nonsmoking group (58.16 ± 49.48 ng/g creatinine).Urinary 1-OHP levels exhibited a significant correlation with BaP yield in cigarette smoke under the Canadian intense smoking condition (y = 3.5563x + 30.171, R (2) = 0.9916, n = 227).

View Article: PubMed Central - PubMed

Affiliation: China National Tobacco Quality Supervision & Test Center, No. 2 Fengyang Street, Zhengzhou, Henan 450001, China.

ABSTRACT
A liquid chromatographic tandem mass spectrometry method (LC-MS/MS) for the simultaneous determination of 1-hydroxypyrene (1-OHP), 3-hydroxybenzo[a]pyrene (3-OHBaP), and 3-hydroxybenz[a]anthracene (3-OHBaA) in human urine has been developed. With the exception of 3-OHBaP at a low spiking level, the average recoveries were greater than 80%. The method has good accuracy (72.1-107.7%) and reproducibility (1.8-11.4%) and was successfully used to study the uptake of pyrene, benzo[a]pyrene, and benzo[a]anthracene from cigarette smoke. The results indicated that urinary 1-OHP concentration in the smoking group (66.58 ± 70.91 ng/g creatinine) was higher than that observed in the nonsmoking group (58.16 ± 49.48 ng/g creatinine). Urinary 3-OHBaA concentrations in nonsmokers and smokers with 8 mg and 10 mg tar cigarettes were 10.98 ± 4.39 ng/g creatinine, 11.01 ± 13.30 ng/g creatinine, and 9.17 ± 12.89 ng/g creatinine, respectively. Urinary 3-OHBaP concentrations in nonsmokers and smokers with 8 mg and 13 mg tar cigarettes were 1.30 ± 0.20 ng/g creatinine, 2.83 ± 1.78 ng/g creatinine, and 6.00 ± 4.44 ng/g creatinine, respectively. Urinary 1-OHP levels exhibited a significant correlation with BaP yield in cigarette smoke under the Canadian intense smoking condition (y = 3.5563x + 30.171, R (2) = 0.9916, n = 227).

No MeSH data available.