Limits...
Development of a method to detect three monohydroxylated polycyclic aromatic hydrocarbons in human urine by liquid chromatographic tandem mass spectrometry.

Zhang X, Hou H, Xiong W, Hu Q - J Anal Methods Chem (2015)

Bottom Line: The method has good accuracy (72.1-107.7%) and reproducibility (1.8-11.4%) and was successfully used to study the uptake of pyrene, benzo[a]pyrene, and benzo[a]anthracene from cigarette smoke.The results indicated that urinary 1-OHP concentration in the smoking group (66.58 ± 70.91 ng/g creatinine) was higher than that observed in the nonsmoking group (58.16 ± 49.48 ng/g creatinine).Urinary 1-OHP levels exhibited a significant correlation with BaP yield in cigarette smoke under the Canadian intense smoking condition (y = 3.5563x + 30.171, R (2) = 0.9916, n = 227).

View Article: PubMed Central - PubMed

Affiliation: China National Tobacco Quality Supervision & Test Center, No. 2 Fengyang Street, Zhengzhou, Henan 450001, China.

ABSTRACT
A liquid chromatographic tandem mass spectrometry method (LC-MS/MS) for the simultaneous determination of 1-hydroxypyrene (1-OHP), 3-hydroxybenzo[a]pyrene (3-OHBaP), and 3-hydroxybenz[a]anthracene (3-OHBaA) in human urine has been developed. With the exception of 3-OHBaP at a low spiking level, the average recoveries were greater than 80%. The method has good accuracy (72.1-107.7%) and reproducibility (1.8-11.4%) and was successfully used to study the uptake of pyrene, benzo[a]pyrene, and benzo[a]anthracene from cigarette smoke. The results indicated that urinary 1-OHP concentration in the smoking group (66.58 ± 70.91 ng/g creatinine) was higher than that observed in the nonsmoking group (58.16 ± 49.48 ng/g creatinine). Urinary 3-OHBaA concentrations in nonsmokers and smokers with 8 mg and 10 mg tar cigarettes were 10.98 ± 4.39 ng/g creatinine, 11.01 ± 13.30 ng/g creatinine, and 9.17 ± 12.89 ng/g creatinine, respectively. Urinary 3-OHBaP concentrations in nonsmokers and smokers with 8 mg and 13 mg tar cigarettes were 1.30 ± 0.20 ng/g creatinine, 2.83 ± 1.78 ng/g creatinine, and 6.00 ± 4.44 ng/g creatinine, respectively. Urinary 1-OHP levels exhibited a significant correlation with BaP yield in cigarette smoke under the Canadian intense smoking condition (y = 3.5563x + 30.171, R (2) = 0.9916, n = 227).

No MeSH data available.


Representative chromatogram from a smoker.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4418005&req=5

fig2: Representative chromatogram from a smoker.

Mentions: To determine the effects of endogenous interferences, the specificity was assessed by comparing the chromatograms of analyte-free human urine samples with those of the spiked urine samples. Under the described chromatographic conditions, the retention times of 1-OHP, 3-OHBaP, 3-OHBaA, and IS were 3.44, 5.94, 4.31, and 3.29 min, respectively. Representative chromatograms from a nonsmoker and smoker are shown in Figures 1 and 2, respectively. No interfering peaks were observed in the chromatograms of the blank urine samples extracted from the nonsmoker. Matrix interference, caused by endogenous materials in the urine samples, was evaluated by comparing the peak areas of spiked standards with those of the standards of pooled blank urine concentrations. The ratios obtained were within the acceptable limits. No significant ion suppressions or enhancements were observed at the expected retention times of the targeted ions.


Development of a method to detect three monohydroxylated polycyclic aromatic hydrocarbons in human urine by liquid chromatographic tandem mass spectrometry.

Zhang X, Hou H, Xiong W, Hu Q - J Anal Methods Chem (2015)

Representative chromatogram from a smoker.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4418005&req=5

fig2: Representative chromatogram from a smoker.
Mentions: To determine the effects of endogenous interferences, the specificity was assessed by comparing the chromatograms of analyte-free human urine samples with those of the spiked urine samples. Under the described chromatographic conditions, the retention times of 1-OHP, 3-OHBaP, 3-OHBaA, and IS were 3.44, 5.94, 4.31, and 3.29 min, respectively. Representative chromatograms from a nonsmoker and smoker are shown in Figures 1 and 2, respectively. No interfering peaks were observed in the chromatograms of the blank urine samples extracted from the nonsmoker. Matrix interference, caused by endogenous materials in the urine samples, was evaluated by comparing the peak areas of spiked standards with those of the standards of pooled blank urine concentrations. The ratios obtained were within the acceptable limits. No significant ion suppressions or enhancements were observed at the expected retention times of the targeted ions.

Bottom Line: The method has good accuracy (72.1-107.7%) and reproducibility (1.8-11.4%) and was successfully used to study the uptake of pyrene, benzo[a]pyrene, and benzo[a]anthracene from cigarette smoke.The results indicated that urinary 1-OHP concentration in the smoking group (66.58 ± 70.91 ng/g creatinine) was higher than that observed in the nonsmoking group (58.16 ± 49.48 ng/g creatinine).Urinary 1-OHP levels exhibited a significant correlation with BaP yield in cigarette smoke under the Canadian intense smoking condition (y = 3.5563x + 30.171, R (2) = 0.9916, n = 227).

View Article: PubMed Central - PubMed

Affiliation: China National Tobacco Quality Supervision & Test Center, No. 2 Fengyang Street, Zhengzhou, Henan 450001, China.

ABSTRACT
A liquid chromatographic tandem mass spectrometry method (LC-MS/MS) for the simultaneous determination of 1-hydroxypyrene (1-OHP), 3-hydroxybenzo[a]pyrene (3-OHBaP), and 3-hydroxybenz[a]anthracene (3-OHBaA) in human urine has been developed. With the exception of 3-OHBaP at a low spiking level, the average recoveries were greater than 80%. The method has good accuracy (72.1-107.7%) and reproducibility (1.8-11.4%) and was successfully used to study the uptake of pyrene, benzo[a]pyrene, and benzo[a]anthracene from cigarette smoke. The results indicated that urinary 1-OHP concentration in the smoking group (66.58 ± 70.91 ng/g creatinine) was higher than that observed in the nonsmoking group (58.16 ± 49.48 ng/g creatinine). Urinary 3-OHBaA concentrations in nonsmokers and smokers with 8 mg and 10 mg tar cigarettes were 10.98 ± 4.39 ng/g creatinine, 11.01 ± 13.30 ng/g creatinine, and 9.17 ± 12.89 ng/g creatinine, respectively. Urinary 3-OHBaP concentrations in nonsmokers and smokers with 8 mg and 13 mg tar cigarettes were 1.30 ± 0.20 ng/g creatinine, 2.83 ± 1.78 ng/g creatinine, and 6.00 ± 4.44 ng/g creatinine, respectively. Urinary 1-OHP levels exhibited a significant correlation with BaP yield in cigarette smoke under the Canadian intense smoking condition (y = 3.5563x + 30.171, R (2) = 0.9916, n = 227).

No MeSH data available.