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Macrophage migration inhibitory factor promoter polymorphisms (-794 CATT 5-8 and -173 G>C): relationship with mRNA expression and soluble MIF levels in young obese subjects.

Matia-García I, Salgado-Goytia L, Muñoz-Valle JF, García-Arellano S, Hernández-Bello J, Salgado-Bernabé AB, Parra-Rojas I - Dis. Markers (2015)

Bottom Line: For both MIF promoter polymorphisms, no significant differences in the genotype and allele frequencies between groups were observed.In addition, we found an increase in MIF mRNA expression in carriers of the 6,6 and C/C genotypes and the 6G haplotype of the -794 CATT5-8 and -173 G>C MIF polymorphisms, although it was not significant.In conclusion, this study found no relationship between obesity and MIF gene promoter polymorphisms with MIF mRNA expression in young obese subjects.

View Article: PubMed Central - PubMed

Affiliation: Laboratorio de Investigación en Obesidad y Diabetes, Unidad Académica de Ciencias Químico Biológicas, Universidad Autónoma de Guerrero, 39090 Chilpancingo, GRO, Mexico.

ABSTRACT
We analyzed the relationship of -794 CATT5-8 and -173 G>C MIF polymorphisms with mRNA and soluble MIF in young obese subjects. A total of 250 young subjects, 150 normal-weight and 100 obese subjects, were recruited in the study. Genotyping of -794 CATT5-8 and -173 G>C MIF polymorphisms was performed by PCR and PCR-RFLP, respectively. MIF mRNA expression was determined by real-time PCR and serum MIF levels were measured using an ELISA kit. For both MIF promoter polymorphisms, no significant differences in the genotype and allele frequencies between groups were observed. MIF mRNA expression was slightly higher in obese subjects than in normal-weight subjects (1.38-fold), while soluble MIF levels did not show differences between groups. In addition, we found an increase in MIF mRNA expression in carriers of the 6,6 and C/C genotypes and the 6G haplotype of the -794 CATT5-8 and -173 G>C MIF polymorphisms, although it was not significant. In conclusion, this study found no relationship between obesity and MIF gene promoter polymorphisms with MIF mRNA expression in young obese subjects.

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Related in: MedlinePlus

Relative MIF mRNA expression by −173 G>C MIF (rs755622) genotypes in normal-weight and obese subjects. (a) The slightly high MIF mRNA expression was observed in the CC carriers in the total population; (b) the GG carriers had lowest expression in both groups. Relative expression analysis was performed using the 2−ΔΔCt method, using GAPDH as the reference gene. Comparison among groups was performed using Mann-Whitney U-test; P < 0.05.
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fig3: Relative MIF mRNA expression by −173 G>C MIF (rs755622) genotypes in normal-weight and obese subjects. (a) The slightly high MIF mRNA expression was observed in the CC carriers in the total population; (b) the GG carriers had lowest expression in both groups. Relative expression analysis was performed using the 2−ΔΔCt method, using GAPDH as the reference gene. Comparison among groups was performed using Mann-Whitney U-test; P < 0.05.

Mentions: Relative MIF mRNA expression in total leucocytes was slightly higher in obese subjects than in normal-weight subjects (1.38-fold) (Figure 1). To investigate the functional impact of both polymorphisms, the quantitative MIF mRNA expression among the different genotypes for both polymorphisms was analyzed. When we analyzed the expression according to the STR −794 CATT5–8 MIF, we found that carriers of the 6,6 genotype had slightly higher expression in comparison to the 7,7 genotype, and the latter with respect to the 5,5 genotype, in the total population (1.38 > 1.08 > 1) (Figure 2(a)). Similarly, when we compared the expression by groups, a modest increase of MIF mRNA expression was observed in the 6,6 carriers in both groups, while the 7,7 carriers had a low expression in the obese group. Additionally, the 6,6 obese carriers expressed slightly higher mRNA expression than normal-weight 6,6 carriers (Figure 2(b)). Carriers of −173 C/C genotype had a slightly higher expression than carriers of the G/G genotype in the total population (1.47 > 1) (Figure 3(a)). When we compared the expression by groups, a modest increase of MIF mRNA expression was observed in the carriers of the C/C genotype compared to the G/G genotype in both groups (Figure 3(b)). To analyze the combined effect of −794 CATT5–8 and −173 G>C MIF polymorphisms on the MIF mRNA expression, we analyzed the expression according to 5G, 6G, and 7C haplotypes. We found that the carriers of the 6G haplotype had the highest expression in comparison to the 7C haplotype, and the latter with respect to the 5G haplotype in the total population (1.38 > 1.21 > 1), although it was not significant (Figure 4(a)). Similarly, in both groups, the carriers of the 6G haplotype had high MIF mRNA expression (Figure 4(b)).


Macrophage migration inhibitory factor promoter polymorphisms (-794 CATT 5-8 and -173 G>C): relationship with mRNA expression and soluble MIF levels in young obese subjects.

Matia-García I, Salgado-Goytia L, Muñoz-Valle JF, García-Arellano S, Hernández-Bello J, Salgado-Bernabé AB, Parra-Rojas I - Dis. Markers (2015)

Relative MIF mRNA expression by −173 G>C MIF (rs755622) genotypes in normal-weight and obese subjects. (a) The slightly high MIF mRNA expression was observed in the CC carriers in the total population; (b) the GG carriers had lowest expression in both groups. Relative expression analysis was performed using the 2−ΔΔCt method, using GAPDH as the reference gene. Comparison among groups was performed using Mann-Whitney U-test; P < 0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4417998&req=5

fig3: Relative MIF mRNA expression by −173 G>C MIF (rs755622) genotypes in normal-weight and obese subjects. (a) The slightly high MIF mRNA expression was observed in the CC carriers in the total population; (b) the GG carriers had lowest expression in both groups. Relative expression analysis was performed using the 2−ΔΔCt method, using GAPDH as the reference gene. Comparison among groups was performed using Mann-Whitney U-test; P < 0.05.
Mentions: Relative MIF mRNA expression in total leucocytes was slightly higher in obese subjects than in normal-weight subjects (1.38-fold) (Figure 1). To investigate the functional impact of both polymorphisms, the quantitative MIF mRNA expression among the different genotypes for both polymorphisms was analyzed. When we analyzed the expression according to the STR −794 CATT5–8 MIF, we found that carriers of the 6,6 genotype had slightly higher expression in comparison to the 7,7 genotype, and the latter with respect to the 5,5 genotype, in the total population (1.38 > 1.08 > 1) (Figure 2(a)). Similarly, when we compared the expression by groups, a modest increase of MIF mRNA expression was observed in the 6,6 carriers in both groups, while the 7,7 carriers had a low expression in the obese group. Additionally, the 6,6 obese carriers expressed slightly higher mRNA expression than normal-weight 6,6 carriers (Figure 2(b)). Carriers of −173 C/C genotype had a slightly higher expression than carriers of the G/G genotype in the total population (1.47 > 1) (Figure 3(a)). When we compared the expression by groups, a modest increase of MIF mRNA expression was observed in the carriers of the C/C genotype compared to the G/G genotype in both groups (Figure 3(b)). To analyze the combined effect of −794 CATT5–8 and −173 G>C MIF polymorphisms on the MIF mRNA expression, we analyzed the expression according to 5G, 6G, and 7C haplotypes. We found that the carriers of the 6G haplotype had the highest expression in comparison to the 7C haplotype, and the latter with respect to the 5G haplotype in the total population (1.38 > 1.21 > 1), although it was not significant (Figure 4(a)). Similarly, in both groups, the carriers of the 6G haplotype had high MIF mRNA expression (Figure 4(b)).

Bottom Line: For both MIF promoter polymorphisms, no significant differences in the genotype and allele frequencies between groups were observed.In addition, we found an increase in MIF mRNA expression in carriers of the 6,6 and C/C genotypes and the 6G haplotype of the -794 CATT5-8 and -173 G>C MIF polymorphisms, although it was not significant.In conclusion, this study found no relationship between obesity and MIF gene promoter polymorphisms with MIF mRNA expression in young obese subjects.

View Article: PubMed Central - PubMed

Affiliation: Laboratorio de Investigación en Obesidad y Diabetes, Unidad Académica de Ciencias Químico Biológicas, Universidad Autónoma de Guerrero, 39090 Chilpancingo, GRO, Mexico.

ABSTRACT
We analyzed the relationship of -794 CATT5-8 and -173 G>C MIF polymorphisms with mRNA and soluble MIF in young obese subjects. A total of 250 young subjects, 150 normal-weight and 100 obese subjects, were recruited in the study. Genotyping of -794 CATT5-8 and -173 G>C MIF polymorphisms was performed by PCR and PCR-RFLP, respectively. MIF mRNA expression was determined by real-time PCR and serum MIF levels were measured using an ELISA kit. For both MIF promoter polymorphisms, no significant differences in the genotype and allele frequencies between groups were observed. MIF mRNA expression was slightly higher in obese subjects than in normal-weight subjects (1.38-fold), while soluble MIF levels did not show differences between groups. In addition, we found an increase in MIF mRNA expression in carriers of the 6,6 and C/C genotypes and the 6G haplotype of the -794 CATT5-8 and -173 G>C MIF polymorphisms, although it was not significant. In conclusion, this study found no relationship between obesity and MIF gene promoter polymorphisms with MIF mRNA expression in young obese subjects.

Show MeSH
Related in: MedlinePlus