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Lactobacillus acidophilus suppresses colitis-associated activation of the IL-23/Th17 axis.

Chen L, Zou Y, Peng J, Lu F, Yin Y, Li F, Yang J - J Immunol Res (2015)

Bottom Line: The aim of this paper is to determine the modulatory effects of Lactobacillus acidophilus on the IL-23/Th17 immune axis in experimental colitis.DSS-induced mouse models of UC were to be saline, hormones, and different concentrations of Lactobacillus acidophilus intervention.And the results showed that administration of L. acidophilus suppressed Th17 cell-mediated secretion of proinflammatory cytokine IL-17 through downregulation of IL-23 and TGFβ1 expression and downstream phosphorylation of p-STAT3.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology, Xiangya Hospital of Central South University, Changsha 410011, China.

ABSTRACT
The aim of this paper is to determine the modulatory effects of Lactobacillus acidophilus on the IL-23/Th17 immune axis in experimental colitis. DSS-induced mouse models of UC were to be saline, hormones, and different concentrations of Lactobacillus acidophilus intervention. The expression of interleukin- (IL-) 17, tumor necrosis factor α (TNFα), IL-23, transforming growth factor β1 (TGFβ1), signal transducer and activator of transcription 3 (STAT3), and phosphorylated (p)-STAT3 was examined by RT-PCR, Western blotting, and immunohistochemical analysis. And the results showed that administration of L. acidophilus suppressed Th17 cell-mediated secretion of proinflammatory cytokine IL-17 through downregulation of IL-23 and TGFβ1 expression and downstream phosphorylation of p-STAT3.

No MeSH data available.


Related in: MedlinePlus

Treatment with Lactobacillus acidophilus reduced TGFβ1 expression in colitis. The mRNA (a) and protein (b) levels of TGFβ1 expression were examined by RT-PCR and Western blotting, respectively, in the colonic tissues of normal control mice group (normal) and DSS-induced mice groups, including L. acidophilus-treated C4–C8 groups, prednisone acetate treated positive control (Pred), and nontreated (Non) and vehicle (Veh) treated control groups. Representative electrophoresis images are shown on the left, and bar graphs presenting mean ± SD (n = 8) values are shown on the right. A plotted trendline chart shows TGFβ1 expression in each group at both mRNA and protein levels (c). aP < 0.05 versus Non, bP < 0.05 versus Veh, cP < 0.05 versus Pred, and dP < 0.05 versus normal.
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fig4: Treatment with Lactobacillus acidophilus reduced TGFβ1 expression in colitis. The mRNA (a) and protein (b) levels of TGFβ1 expression were examined by RT-PCR and Western blotting, respectively, in the colonic tissues of normal control mice group (normal) and DSS-induced mice groups, including L. acidophilus-treated C4–C8 groups, prednisone acetate treated positive control (Pred), and nontreated (Non) and vehicle (Veh) treated control groups. Representative electrophoresis images are shown on the left, and bar graphs presenting mean ± SD (n = 8) values are shown on the right. A plotted trendline chart shows TGFβ1 expression in each group at both mRNA and protein levels (c). aP < 0.05 versus Non, bP < 0.05 versus Veh, cP < 0.05 versus Pred, and dP < 0.05 versus normal.

Mentions: IL-23 and TGFβ1 have been shown to play essential roles in promoting Th17 cell differentiation, expansion, and function [9, 14, 17, 18]. We tested whether the inhibitory effect of L. acidophilus on Th17-associated cytokine expression is achieved through downregulation of IL-23 and/or TGFβ1 expression, thereby suppressing Th17 cell differentiation and stabilization. Interestingly, treatment of colitis with a lower dose of L. acidophilus (C4–C6 groups) caused a dramatic decrease in IL-23 mRNA expression, which reached a level comparable to that of the normal control group (Figure 3(a)). Moreover, all L. acidophilus-treated groups showed a significant (P < 0.05) decrease in IL-23 protein expression (Figure 3(b)). Notably, both the mRNA and protein levels of IL-23 expression were lowest in group C5 among all the colitis groups (Figure 3(c)), at a level similar to that observed for IL-17 expression. Likewise, L. acidophilus administration at all doses induced marked inhibition of TGFβ1 mRNA expression (Figure 4(a)). A dramatic attenuation of TGFβ1 protein expression was also observed in the low dose groups, C4 and C5 (Figure 4(b)). Again, mice treated with 105 CFU/10 g L. acidophilus, group C5, showed the lowest expression of TGFβ1 (Figure 4(c)).


Lactobacillus acidophilus suppresses colitis-associated activation of the IL-23/Th17 axis.

Chen L, Zou Y, Peng J, Lu F, Yin Y, Li F, Yang J - J Immunol Res (2015)

Treatment with Lactobacillus acidophilus reduced TGFβ1 expression in colitis. The mRNA (a) and protein (b) levels of TGFβ1 expression were examined by RT-PCR and Western blotting, respectively, in the colonic tissues of normal control mice group (normal) and DSS-induced mice groups, including L. acidophilus-treated C4–C8 groups, prednisone acetate treated positive control (Pred), and nontreated (Non) and vehicle (Veh) treated control groups. Representative electrophoresis images are shown on the left, and bar graphs presenting mean ± SD (n = 8) values are shown on the right. A plotted trendline chart shows TGFβ1 expression in each group at both mRNA and protein levels (c). aP < 0.05 versus Non, bP < 0.05 versus Veh, cP < 0.05 versus Pred, and dP < 0.05 versus normal.
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4417982&req=5

fig4: Treatment with Lactobacillus acidophilus reduced TGFβ1 expression in colitis. The mRNA (a) and protein (b) levels of TGFβ1 expression were examined by RT-PCR and Western blotting, respectively, in the colonic tissues of normal control mice group (normal) and DSS-induced mice groups, including L. acidophilus-treated C4–C8 groups, prednisone acetate treated positive control (Pred), and nontreated (Non) and vehicle (Veh) treated control groups. Representative electrophoresis images are shown on the left, and bar graphs presenting mean ± SD (n = 8) values are shown on the right. A plotted trendline chart shows TGFβ1 expression in each group at both mRNA and protein levels (c). aP < 0.05 versus Non, bP < 0.05 versus Veh, cP < 0.05 versus Pred, and dP < 0.05 versus normal.
Mentions: IL-23 and TGFβ1 have been shown to play essential roles in promoting Th17 cell differentiation, expansion, and function [9, 14, 17, 18]. We tested whether the inhibitory effect of L. acidophilus on Th17-associated cytokine expression is achieved through downregulation of IL-23 and/or TGFβ1 expression, thereby suppressing Th17 cell differentiation and stabilization. Interestingly, treatment of colitis with a lower dose of L. acidophilus (C4–C6 groups) caused a dramatic decrease in IL-23 mRNA expression, which reached a level comparable to that of the normal control group (Figure 3(a)). Moreover, all L. acidophilus-treated groups showed a significant (P < 0.05) decrease in IL-23 protein expression (Figure 3(b)). Notably, both the mRNA and protein levels of IL-23 expression were lowest in group C5 among all the colitis groups (Figure 3(c)), at a level similar to that observed for IL-17 expression. Likewise, L. acidophilus administration at all doses induced marked inhibition of TGFβ1 mRNA expression (Figure 4(a)). A dramatic attenuation of TGFβ1 protein expression was also observed in the low dose groups, C4 and C5 (Figure 4(b)). Again, mice treated with 105 CFU/10 g L. acidophilus, group C5, showed the lowest expression of TGFβ1 (Figure 4(c)).

Bottom Line: The aim of this paper is to determine the modulatory effects of Lactobacillus acidophilus on the IL-23/Th17 immune axis in experimental colitis.DSS-induced mouse models of UC were to be saline, hormones, and different concentrations of Lactobacillus acidophilus intervention.And the results showed that administration of L. acidophilus suppressed Th17 cell-mediated secretion of proinflammatory cytokine IL-17 through downregulation of IL-23 and TGFβ1 expression and downstream phosphorylation of p-STAT3.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology, Xiangya Hospital of Central South University, Changsha 410011, China.

ABSTRACT
The aim of this paper is to determine the modulatory effects of Lactobacillus acidophilus on the IL-23/Th17 immune axis in experimental colitis. DSS-induced mouse models of UC were to be saline, hormones, and different concentrations of Lactobacillus acidophilus intervention. The expression of interleukin- (IL-) 17, tumor necrosis factor α (TNFα), IL-23, transforming growth factor β1 (TGFβ1), signal transducer and activator of transcription 3 (STAT3), and phosphorylated (p)-STAT3 was examined by RT-PCR, Western blotting, and immunohistochemical analysis. And the results showed that administration of L. acidophilus suppressed Th17 cell-mediated secretion of proinflammatory cytokine IL-17 through downregulation of IL-23 and TGFβ1 expression and downstream phosphorylation of p-STAT3.

No MeSH data available.


Related in: MedlinePlus