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Lactobacillus acidophilus suppresses colitis-associated activation of the IL-23/Th17 axis.

Chen L, Zou Y, Peng J, Lu F, Yin Y, Li F, Yang J - J Immunol Res (2015)

Bottom Line: The aim of this paper is to determine the modulatory effects of Lactobacillus acidophilus on the IL-23/Th17 immune axis in experimental colitis.DSS-induced mouse models of UC were to be saline, hormones, and different concentrations of Lactobacillus acidophilus intervention.And the results showed that administration of L. acidophilus suppressed Th17 cell-mediated secretion of proinflammatory cytokine IL-17 through downregulation of IL-23 and TGFβ1 expression and downstream phosphorylation of p-STAT3.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology, Xiangya Hospital of Central South University, Changsha 410011, China.

ABSTRACT
The aim of this paper is to determine the modulatory effects of Lactobacillus acidophilus on the IL-23/Th17 immune axis in experimental colitis. DSS-induced mouse models of UC were to be saline, hormones, and different concentrations of Lactobacillus acidophilus intervention. The expression of interleukin- (IL-) 17, tumor necrosis factor α (TNFα), IL-23, transforming growth factor β1 (TGFβ1), signal transducer and activator of transcription 3 (STAT3), and phosphorylated (p)-STAT3 was examined by RT-PCR, Western blotting, and immunohistochemical analysis. And the results showed that administration of L. acidophilus suppressed Th17 cell-mediated secretion of proinflammatory cytokine IL-17 through downregulation of IL-23 and TGFβ1 expression and downstream phosphorylation of p-STAT3.

No MeSH data available.


Related in: MedlinePlus

Lactobacillus acidophilus treatment suppressed TNFα expression in colitis. The mRNA (a) and protein (b) levels of TNFα expression were examined by RT-PCR and Western blotting, respectively, in the colonic tissues of normal control mice group (normal) and DSS-induced mice groups, including L. acidophilus-treated C4–C8 groups, prednisone acetate treated positive control (Pred), and nontreated (Non) and vehicle (Veh) treated control groups. Representative electrophoresis images are shown on the left, and bar graphs presenting mean ± SD (n = 8) values are shown on the right. A plotted trendline chart shows TNFα expression in each group at both mRNA and protein levels (c). aP < 0.05 versus Non, bP < 0.05 versus Veh, cP < 0.05 versus Pred, and dP < 0.05 versus normal.
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Related In: Results  -  Collection


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fig2: Lactobacillus acidophilus treatment suppressed TNFα expression in colitis. The mRNA (a) and protein (b) levels of TNFα expression were examined by RT-PCR and Western blotting, respectively, in the colonic tissues of normal control mice group (normal) and DSS-induced mice groups, including L. acidophilus-treated C4–C8 groups, prednisone acetate treated positive control (Pred), and nontreated (Non) and vehicle (Veh) treated control groups. Representative electrophoresis images are shown on the left, and bar graphs presenting mean ± SD (n = 8) values are shown on the right. A plotted trendline chart shows TNFα expression in each group at both mRNA and protein levels (c). aP < 0.05 versus Non, bP < 0.05 versus Veh, cP < 0.05 versus Pred, and dP < 0.05 versus normal.

Mentions: Because TNFα is another important proinflammatory cytokine secreted by Th17 cells, we also examined the effect of L. acidophilus treatment on TNFα expression. Similarly to IL-17, TNFα expression at both the mRNA and protein levels was significantly (P < 0.05) decreased by L. acidophilus treatment in groups C4–C8 compared to the vehicle treated control group (Figures 2(a) and 2(b)). However, unlike IL-17, TNFα expression was lowest in Group C6 among all the DSS-treated groups (Figure 2(c)).


Lactobacillus acidophilus suppresses colitis-associated activation of the IL-23/Th17 axis.

Chen L, Zou Y, Peng J, Lu F, Yin Y, Li F, Yang J - J Immunol Res (2015)

Lactobacillus acidophilus treatment suppressed TNFα expression in colitis. The mRNA (a) and protein (b) levels of TNFα expression were examined by RT-PCR and Western blotting, respectively, in the colonic tissues of normal control mice group (normal) and DSS-induced mice groups, including L. acidophilus-treated C4–C8 groups, prednisone acetate treated positive control (Pred), and nontreated (Non) and vehicle (Veh) treated control groups. Representative electrophoresis images are shown on the left, and bar graphs presenting mean ± SD (n = 8) values are shown on the right. A plotted trendline chart shows TNFα expression in each group at both mRNA and protein levels (c). aP < 0.05 versus Non, bP < 0.05 versus Veh, cP < 0.05 versus Pred, and dP < 0.05 versus normal.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4417982&req=5

fig2: Lactobacillus acidophilus treatment suppressed TNFα expression in colitis. The mRNA (a) and protein (b) levels of TNFα expression were examined by RT-PCR and Western blotting, respectively, in the colonic tissues of normal control mice group (normal) and DSS-induced mice groups, including L. acidophilus-treated C4–C8 groups, prednisone acetate treated positive control (Pred), and nontreated (Non) and vehicle (Veh) treated control groups. Representative electrophoresis images are shown on the left, and bar graphs presenting mean ± SD (n = 8) values are shown on the right. A plotted trendline chart shows TNFα expression in each group at both mRNA and protein levels (c). aP < 0.05 versus Non, bP < 0.05 versus Veh, cP < 0.05 versus Pred, and dP < 0.05 versus normal.
Mentions: Because TNFα is another important proinflammatory cytokine secreted by Th17 cells, we also examined the effect of L. acidophilus treatment on TNFα expression. Similarly to IL-17, TNFα expression at both the mRNA and protein levels was significantly (P < 0.05) decreased by L. acidophilus treatment in groups C4–C8 compared to the vehicle treated control group (Figures 2(a) and 2(b)). However, unlike IL-17, TNFα expression was lowest in Group C6 among all the DSS-treated groups (Figure 2(c)).

Bottom Line: The aim of this paper is to determine the modulatory effects of Lactobacillus acidophilus on the IL-23/Th17 immune axis in experimental colitis.DSS-induced mouse models of UC were to be saline, hormones, and different concentrations of Lactobacillus acidophilus intervention.And the results showed that administration of L. acidophilus suppressed Th17 cell-mediated secretion of proinflammatory cytokine IL-17 through downregulation of IL-23 and TGFβ1 expression and downstream phosphorylation of p-STAT3.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology, Xiangya Hospital of Central South University, Changsha 410011, China.

ABSTRACT
The aim of this paper is to determine the modulatory effects of Lactobacillus acidophilus on the IL-23/Th17 immune axis in experimental colitis. DSS-induced mouse models of UC were to be saline, hormones, and different concentrations of Lactobacillus acidophilus intervention. The expression of interleukin- (IL-) 17, tumor necrosis factor α (TNFα), IL-23, transforming growth factor β1 (TGFβ1), signal transducer and activator of transcription 3 (STAT3), and phosphorylated (p)-STAT3 was examined by RT-PCR, Western blotting, and immunohistochemical analysis. And the results showed that administration of L. acidophilus suppressed Th17 cell-mediated secretion of proinflammatory cytokine IL-17 through downregulation of IL-23 and TGFβ1 expression and downstream phosphorylation of p-STAT3.

No MeSH data available.


Related in: MedlinePlus