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The effects of tempol on cyclophosphamide-induced oxidative stress in rat micturition reflexes.

Gonzalez EJ, Peterson A, Malley S, Daniel M, Lambert D, Kosofsky M, Vizzard MA - ScientificWorldJournal (2015)

Bottom Line: CYP treatment increased ATP, Sub P, and CGRP expression in the urinary bladder and cystometric fluid.In CYP-treated rats, Tempol significantly (P ≤ 0.01) increased bladder capacity and reduced voiding frequency compared to CYP-treated rats without Tempol.Tempol significantly (P ≤ 0.01) reduced ATP expression, 3-NT, and ROS/RNS expression in the urinary tract of CYP-treated rats.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurological Sciences, University of Vermont College of Medicine, Burlington, VT 05405, USA.

ABSTRACT
We hypothesized that cyclophosphamide- (CYP-) induced cystitis results in oxidative stress and contributes to urinary bladder dysfunction. We determined (1) the expression of oxidative stress markers 3-nitrotyrosine (3-NT), reactive oxygen species (ROS)/reactive nitrogen species (RNS), inflammatory modulators, neuropeptides calcitonin gene-related peptide (CGRP), substance P (Sub P), and adenosine triphosphate (ATP) that contribute to the inflammatory process in the urinary tract and (2) the functional role of oxidative stress in urinary bladder dysfunction with an antioxidant, Tempol, (1 mM in drinking water) combined with conscious cystometry. In CYP-treated (4 hr or 48 hr; 150 mg/kg, i.p.) rats, ROS/RNS and 3-NT significantly (P ≤ 0.01) increased in urinary bladder. CYP treatment increased ATP, Sub P, and CGRP expression in the urinary bladder and cystometric fluid. In CYP-treated rats, Tempol significantly (P ≤ 0.01) increased bladder capacity and reduced voiding frequency compared to CYP-treated rats without Tempol. Tempol significantly (P ≤ 0.01) reduced ATP expression, 3-NT, and ROS/RNS expression in the urinary tract of CYP-treated rats. These studies demonstrate that reducing oxidative stress in CYP-induced cystitis improves urinary bladder function and reduces markers of oxidative stress and inflammation.

No MeSH data available.


Related in: MedlinePlus

Tempol in the drinking water was without effect on baseline pressure ((a); BP), threshold pressure ((b); TP), or peak micturition pressure ((c); MP; cm H2O) in control or CYP-treated (4 hr; 48 hr) rats. Sample sizes are n = 6 in control and treatment groups. #P ≤ 0.01 compared to control + vehicle (between-group difference).
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fig6: Tempol in the drinking water was without effect on baseline pressure ((a); BP), threshold pressure ((b); TP), or peak micturition pressure ((c); MP; cm H2O) in control or CYP-treated (4 hr; 48 hr) rats. Sample sizes are n = 6 in control and treatment groups. #P ≤ 0.01 compared to control + vehicle (between-group difference).

Mentions: Tempol (1 mM) treatment had no effects on ICI, BC, or VV in control rats (no CYP treatment) compared with control rats (no CYP treatment) treated with vehicle (Figure 5). There were no changes in BP, TP, or peak MP with Tempol treatment compared to control rats (no CYP treatment) treated with vehicle (Figure 6). Residual volume in control rats with or without Tempol (vehicle) treatment was minimal (≤10 μL).


The effects of tempol on cyclophosphamide-induced oxidative stress in rat micturition reflexes.

Gonzalez EJ, Peterson A, Malley S, Daniel M, Lambert D, Kosofsky M, Vizzard MA - ScientificWorldJournal (2015)

Tempol in the drinking water was without effect on baseline pressure ((a); BP), threshold pressure ((b); TP), or peak micturition pressure ((c); MP; cm H2O) in control or CYP-treated (4 hr; 48 hr) rats. Sample sizes are n = 6 in control and treatment groups. #P ≤ 0.01 compared to control + vehicle (between-group difference).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4417973&req=5

fig6: Tempol in the drinking water was without effect on baseline pressure ((a); BP), threshold pressure ((b); TP), or peak micturition pressure ((c); MP; cm H2O) in control or CYP-treated (4 hr; 48 hr) rats. Sample sizes are n = 6 in control and treatment groups. #P ≤ 0.01 compared to control + vehicle (between-group difference).
Mentions: Tempol (1 mM) treatment had no effects on ICI, BC, or VV in control rats (no CYP treatment) compared with control rats (no CYP treatment) treated with vehicle (Figure 5). There were no changes in BP, TP, or peak MP with Tempol treatment compared to control rats (no CYP treatment) treated with vehicle (Figure 6). Residual volume in control rats with or without Tempol (vehicle) treatment was minimal (≤10 μL).

Bottom Line: CYP treatment increased ATP, Sub P, and CGRP expression in the urinary bladder and cystometric fluid.In CYP-treated rats, Tempol significantly (P ≤ 0.01) increased bladder capacity and reduced voiding frequency compared to CYP-treated rats without Tempol.Tempol significantly (P ≤ 0.01) reduced ATP expression, 3-NT, and ROS/RNS expression in the urinary tract of CYP-treated rats.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurological Sciences, University of Vermont College of Medicine, Burlington, VT 05405, USA.

ABSTRACT
We hypothesized that cyclophosphamide- (CYP-) induced cystitis results in oxidative stress and contributes to urinary bladder dysfunction. We determined (1) the expression of oxidative stress markers 3-nitrotyrosine (3-NT), reactive oxygen species (ROS)/reactive nitrogen species (RNS), inflammatory modulators, neuropeptides calcitonin gene-related peptide (CGRP), substance P (Sub P), and adenosine triphosphate (ATP) that contribute to the inflammatory process in the urinary tract and (2) the functional role of oxidative stress in urinary bladder dysfunction with an antioxidant, Tempol, (1 mM in drinking water) combined with conscious cystometry. In CYP-treated (4 hr or 48 hr; 150 mg/kg, i.p.) rats, ROS/RNS and 3-NT significantly (P ≤ 0.01) increased in urinary bladder. CYP treatment increased ATP, Sub P, and CGRP expression in the urinary bladder and cystometric fluid. In CYP-treated rats, Tempol significantly (P ≤ 0.01) increased bladder capacity and reduced voiding frequency compared to CYP-treated rats without Tempol. Tempol significantly (P ≤ 0.01) reduced ATP expression, 3-NT, and ROS/RNS expression in the urinary tract of CYP-treated rats. These studies demonstrate that reducing oxidative stress in CYP-induced cystitis improves urinary bladder function and reduces markers of oxidative stress and inflammation.

No MeSH data available.


Related in: MedlinePlus