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Dietary L-arginine supplementation protects weanling pigs from deoxynivalenol-induced toxicity.

Wu L, Liao P, He L, Feng Z, Ren W, Yin J, Duan J, Li T, Yin Y - Toxins (Basel) (2015)

Bottom Line: However, clinical parameters, including jejunal morphology, amino acid concentrations in the serum, jejunum and ileum, were improved by Arg (p < 0.05).Furthermore, the mRNA levels for sodium-glucose transporter-1 (SGLT-1), glucose transporter type-2 (GLUT-2) and y(+)L-type amino acid transporter-1 (y(+)LAT-1) were downregulated in the DON group, but the values were increased in the DON + ARG group (p < 0.05).Collectively, these results indicate that dietary supplementation with Arg exerts a protective role in pigs fed DON-contaminated diets.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, Hunan, China. adonis5@163.com.

ABSTRACT
This study was conducted to determine the positive effects of dietary supplementation with L-arginine (Arg) on piglets fed a deoxynivalenol (DON)-contaminated diet. A total of eighteen, 28-day-old healthy weanling pigs were randomly assigned into one of three groups: uncontaminated basal diet (control group), 6 mg/kg DON-contaminated diet (DON group) and 6 mg/kg DON + 1% L-arginine (DON + ARG group). After 21 days of Arg supplementation, piglets in the DON and DON + ARG groups were challenged by feeding 6 mg/kg DON-contaminated diet for seven days. The results showed that DON resulted in damage to piglets. However, clinical parameters, including jejunal morphology, amino acid concentrations in the serum, jejunum and ileum, were improved by Arg (p < 0.05). Furthermore, the mRNA levels for sodium-glucose transporter-1 (SGLT-1), glucose transporter type-2 (GLUT-2) and y(+)L-type amino acid transporter-1 (y(+)LAT-1) were downregulated in the DON group, but the values were increased in the DON + ARG group (p < 0.05). Collectively, these results indicate that dietary supplementation with Arg exerts a protective role in pigs fed DON-contaminated diets.

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Related in: MedlinePlus

Effects of DON exposure on histopathology in the jejunum of weanling pigs: (A) normal histological structure in pigs fed the control diet; (B) severely impaired enterocytes in pigs fed the 6 mg/kg DON diet; (C) mild impairment of enterocytes in pigs fed the 6 mg/kg DON + 1% arginine diet. Original magnification: 400× (n = 6). All the scale bars in figures A to C represent 100 µM.
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toxins-07-01341-f001: Effects of DON exposure on histopathology in the jejunum of weanling pigs: (A) normal histological structure in pigs fed the control diet; (B) severely impaired enterocytes in pigs fed the 6 mg/kg DON diet; (C) mild impairment of enterocytes in pigs fed the 6 mg/kg DON + 1% arginine diet. Original magnification: 400× (n = 6). All the scale bars in figures A to C represent 100 µM.

Mentions: Pathological changes (Table 5) and deformation of enterocytes (Figure 1) in the mucosa were observed after the DON-infected diet was fed to pigs. Of note, only slight changes were found in the DON + ARG group. The villus height in the DON group was significantly lower than the other groups. The decrease in the DON + ARG group was mild. No difference was found between the DON + ARG and control groups (p < 0.05). No significant changes were found in crypt depth among the three groups of pigs. The ratio of villus height to crypt depth in the DON group was the lowest. This ratio in the DON + ARG group was slightly decreased compared to the control group (p > 0.05).


Dietary L-arginine supplementation protects weanling pigs from deoxynivalenol-induced toxicity.

Wu L, Liao P, He L, Feng Z, Ren W, Yin J, Duan J, Li T, Yin Y - Toxins (Basel) (2015)

Effects of DON exposure on histopathology in the jejunum of weanling pigs: (A) normal histological structure in pigs fed the control diet; (B) severely impaired enterocytes in pigs fed the 6 mg/kg DON diet; (C) mild impairment of enterocytes in pigs fed the 6 mg/kg DON + 1% arginine diet. Original magnification: 400× (n = 6). All the scale bars in figures A to C represent 100 µM.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4417970&req=5

toxins-07-01341-f001: Effects of DON exposure on histopathology in the jejunum of weanling pigs: (A) normal histological structure in pigs fed the control diet; (B) severely impaired enterocytes in pigs fed the 6 mg/kg DON diet; (C) mild impairment of enterocytes in pigs fed the 6 mg/kg DON + 1% arginine diet. Original magnification: 400× (n = 6). All the scale bars in figures A to C represent 100 µM.
Mentions: Pathological changes (Table 5) and deformation of enterocytes (Figure 1) in the mucosa were observed after the DON-infected diet was fed to pigs. Of note, only slight changes were found in the DON + ARG group. The villus height in the DON group was significantly lower than the other groups. The decrease in the DON + ARG group was mild. No difference was found between the DON + ARG and control groups (p < 0.05). No significant changes were found in crypt depth among the three groups of pigs. The ratio of villus height to crypt depth in the DON group was the lowest. This ratio in the DON + ARG group was slightly decreased compared to the control group (p > 0.05).

Bottom Line: However, clinical parameters, including jejunal morphology, amino acid concentrations in the serum, jejunum and ileum, were improved by Arg (p < 0.05).Furthermore, the mRNA levels for sodium-glucose transporter-1 (SGLT-1), glucose transporter type-2 (GLUT-2) and y(+)L-type amino acid transporter-1 (y(+)LAT-1) were downregulated in the DON group, but the values were increased in the DON + ARG group (p < 0.05).Collectively, these results indicate that dietary supplementation with Arg exerts a protective role in pigs fed DON-contaminated diets.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, Hunan, China. adonis5@163.com.

ABSTRACT
This study was conducted to determine the positive effects of dietary supplementation with L-arginine (Arg) on piglets fed a deoxynivalenol (DON)-contaminated diet. A total of eighteen, 28-day-old healthy weanling pigs were randomly assigned into one of three groups: uncontaminated basal diet (control group), 6 mg/kg DON-contaminated diet (DON group) and 6 mg/kg DON + 1% L-arginine (DON + ARG group). After 21 days of Arg supplementation, piglets in the DON and DON + ARG groups were challenged by feeding 6 mg/kg DON-contaminated diet for seven days. The results showed that DON resulted in damage to piglets. However, clinical parameters, including jejunal morphology, amino acid concentrations in the serum, jejunum and ileum, were improved by Arg (p < 0.05). Furthermore, the mRNA levels for sodium-glucose transporter-1 (SGLT-1), glucose transporter type-2 (GLUT-2) and y(+)L-type amino acid transporter-1 (y(+)LAT-1) were downregulated in the DON group, but the values were increased in the DON + ARG group (p < 0.05). Collectively, these results indicate that dietary supplementation with Arg exerts a protective role in pigs fed DON-contaminated diets.

Show MeSH
Related in: MedlinePlus