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Influence of different shellfish matrices on the separation of PSP toxins using a postcolumn oxidation liquid chromatography method.

Rey V, Alfonso A, Botana LM, Botana AM - Toxins (Basel) (2015)

Bottom Line: The matrix peaks are not always the same, which is a significant issue when it comes to producing good, reliable results regarding resolution and toxicity information.Scallop and oyster matrices needed a decrease in the concentration of heptane sulfonate to separate GTX4 from matrix peaks, as well as dcGTX3 for oysters, with a concentration of 6.5 mM for solvent A and 6.25 mM for solvent B.Also, for scallops and oysters, matrix interferences depend not only on the sampling site but also on the date of collection as well as the species; for mussels and clams, differences are noted only when the sampling site varies.

View Article: PubMed Central - PubMed

Affiliation: Department of Analytical Chemistry, Science Faculty, University of Santiago de Compostela, Lugo 27002, Spain. veronica.rey@rai.usc.es.

ABSTRACT
The separation of PSP toxins using liquid chromatography with a post-column oxidation fluorescence detection method was performed with different matrices. The separation of PSP toxins depends on several factors, and it is crucial to take into account the presence of interfering matrix peaks to produce a good separation. The matrix peaks are not always the same, which is a significant issue when it comes to producing good, reliable results regarding resolution and toxicity information. Different real shellfish matrices (mussel, scallop, clam and oyster) were studied, and it was seen that the interference is not the same for each individual matrix. It also depends on the species, sampling location and the date of collection. It was proposed that separation should be accomplished taking into account the type of matrix, as well as the concentration of heptane sulfonate in both solvents, since the mobile phase varies regarding the matrix. Scallop and oyster matrices needed a decrease in the concentration of heptane sulfonate to separate GTX4 from matrix peaks, as well as dcGTX3 for oysters, with a concentration of 6.5 mM for solvent A and 6.25 mM for solvent B. For mussel and clam matrices, interfering peaks are not as large as they are in the other group, and the heptane sulfonate concentration was 8.25 mM for both solvents. Also, for scallops and oysters, matrix interferences depend not only on the sampling site but also on the date of collection as well as the species; for mussels and clams, differences are noted only when the sampling site varies.

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Related in: MedlinePlus

Overlaid chromatographic separation, showing temporal variations for: (a) Sample PSP8 and (b) sample PSP7.
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toxins-07-01324-f006: Overlaid chromatographic separation, showing temporal variations for: (a) Sample PSP8 and (b) sample PSP7.

Mentions: In the analyzed scallop samples, this matrix peak was higher than in the scallops used as blanks, and this might be because they are different species. However, when comparing scallop samples which belong to the same species, it is observed that there are also differences. This is the case for samples PSP7 and PSP8; both are from the same area with a sampling difference of two months, and it was observed that the peaks due to the matrix are different despite being from the same area, so it seems that temporal variation is an important factor. Figure 6 shows the chromatograms of both samples, where the matrix peak in (a) (sample PSP8) is lower than in (b) (sample PSP7) and in this case that peak is the reason why the toxins are not seen in the scale shown in the chromatograms. When comparing samples from different areas it was seen that they also give different matrix peaks, so these differences in matrix peaks depend on the species, date of collection and geographical area. The reason might be that at different moments the marine streams and sea temperatures are different, which influences the presence of microorganisms and then algae blooms.


Influence of different shellfish matrices on the separation of PSP toxins using a postcolumn oxidation liquid chromatography method.

Rey V, Alfonso A, Botana LM, Botana AM - Toxins (Basel) (2015)

Overlaid chromatographic separation, showing temporal variations for: (a) Sample PSP8 and (b) sample PSP7.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4417969&req=5

toxins-07-01324-f006: Overlaid chromatographic separation, showing temporal variations for: (a) Sample PSP8 and (b) sample PSP7.
Mentions: In the analyzed scallop samples, this matrix peak was higher than in the scallops used as blanks, and this might be because they are different species. However, when comparing scallop samples which belong to the same species, it is observed that there are also differences. This is the case for samples PSP7 and PSP8; both are from the same area with a sampling difference of two months, and it was observed that the peaks due to the matrix are different despite being from the same area, so it seems that temporal variation is an important factor. Figure 6 shows the chromatograms of both samples, where the matrix peak in (a) (sample PSP8) is lower than in (b) (sample PSP7) and in this case that peak is the reason why the toxins are not seen in the scale shown in the chromatograms. When comparing samples from different areas it was seen that they also give different matrix peaks, so these differences in matrix peaks depend on the species, date of collection and geographical area. The reason might be that at different moments the marine streams and sea temperatures are different, which influences the presence of microorganisms and then algae blooms.

Bottom Line: The matrix peaks are not always the same, which is a significant issue when it comes to producing good, reliable results regarding resolution and toxicity information.Scallop and oyster matrices needed a decrease in the concentration of heptane sulfonate to separate GTX4 from matrix peaks, as well as dcGTX3 for oysters, with a concentration of 6.5 mM for solvent A and 6.25 mM for solvent B.Also, for scallops and oysters, matrix interferences depend not only on the sampling site but also on the date of collection as well as the species; for mussels and clams, differences are noted only when the sampling site varies.

View Article: PubMed Central - PubMed

Affiliation: Department of Analytical Chemistry, Science Faculty, University of Santiago de Compostela, Lugo 27002, Spain. veronica.rey@rai.usc.es.

ABSTRACT
The separation of PSP toxins using liquid chromatography with a post-column oxidation fluorescence detection method was performed with different matrices. The separation of PSP toxins depends on several factors, and it is crucial to take into account the presence of interfering matrix peaks to produce a good separation. The matrix peaks are not always the same, which is a significant issue when it comes to producing good, reliable results regarding resolution and toxicity information. Different real shellfish matrices (mussel, scallop, clam and oyster) were studied, and it was seen that the interference is not the same for each individual matrix. It also depends on the species, sampling location and the date of collection. It was proposed that separation should be accomplished taking into account the type of matrix, as well as the concentration of heptane sulfonate in both solvents, since the mobile phase varies regarding the matrix. Scallop and oyster matrices needed a decrease in the concentration of heptane sulfonate to separate GTX4 from matrix peaks, as well as dcGTX3 for oysters, with a concentration of 6.5 mM for solvent A and 6.25 mM for solvent B. For mussel and clam matrices, interfering peaks are not as large as they are in the other group, and the heptane sulfonate concentration was 8.25 mM for both solvents. Also, for scallops and oysters, matrix interferences depend not only on the sampling site but also on the date of collection as well as the species; for mussels and clams, differences are noted only when the sampling site varies.

Show MeSH
Related in: MedlinePlus